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Aeromonas cainea W3 capable of degrading typical fluoroquinolone antibiotics and application thereof

A technology of Aeromonas guinea pig, fluoroquinolones, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc.

Active Publication Date: 2022-02-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no literature report that Aeromonas caviae has the ability to degrade fluoroquinolone antibiotics

Method used

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  • Aeromonas cainea W3 capable of degrading typical fluoroquinolone antibiotics and application thereof
  • Aeromonas cainea W3 capable of degrading typical fluoroquinolone antibiotics and application thereof
  • Aeromonas cainea W3 capable of degrading typical fluoroquinolone antibiotics and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and identification of Aeromonas caviae (Aeromonas caviae) W3

[0034] 1. Enrichment and isolation of strains

[0035] Take the aerobic sludge from Guangzhou Lijiao Sewage Treatment Plant and wash it according to the following steps: ①Sieve out large particles, let it settle for 30 minutes, and discard the supernatant; ②Add PBS (phosphate buffer saline) solution for resuspension, and vortex for 10 minutes , discard the supernatant. Take 20mL pure mud and add 200mL nutrient solution (see Table 1 for composition and content), add norfloxacin (NOR) to 10mg / L, sodium acetate 0.3g / L. Take 0.1mL bacterial suspension (diluted 10 1 ~10 4 fold) spread on the MSM solid plate medium with norfloxacin as the only carbon source and culture in the dark at 37°C until a single pure colony appears on the plate. Colonies with good growth status and regular edges were selected for multiple purifications. The MSM medium contains norfloxacin 0.05g / L, agar powder 1.5g / ...

Embodiment 2

[0053] Example 2 Degradation test of Aeromonas caviae (Aeromonas caviae) W3 to norfloxacin (NOR) under aerobic conditions

[0054] 1. Carry out the Erlenmeyer flask experiment of norfloxacin degradation to the Aeromonas caviae (Aeromonas caviae) W3 of embodiment 1 separation and purification under aerobic conditions, detect the degradation rate of norfloxacin and the growth situation OD600 of bacterial strain . Taking temperature (10-40°C), pH (6-8) and initial OD600 (0.2-0.8) as independent variables, and degradation rate as dependent variable, a three-factor three-level response surface analysis was carried out. The design scheme was Box-Behnken. The joint model is Quadratic. The experimental data are shown in the table below:

[0055] Table 1 Response surface data results

[0056]

[0057]

[0058] 2. Under the condition of using norfloxacin as a single carbon source, the growth of Aeromonas caviae (Aeromonas caviae) showed a general downward trend in OD600. Corre...

Embodiment 3

[0061] The influence of different fluoroquinolone antibiotics of embodiment 3 on guinea pig Aeromonas (Aeromonas caviae) W3 growth

[0062] In the Erlenmeyer flask that 100mL embodiment 1 described nutrient solution is housed, add four kinds of fluoroquinolone antibiotic medicines respectively, make the initial concentration of norfloxacin (NOR) be 75mg / L respectively, ciprofloxacin ( The initial concentration of CIP) was 65mg / L, the initial concentration of enrofloxacin (ENR) was 37.5mg / L, and the initial concentration of ofloxacin (OFL) was 75mg / L. Take the bacterial solution in the logarithmic growth phase and centrifuge at 6000rpm at 4°C for 6 minutes, discard the supernatant, add an appropriate amount of PBS solution for washing, and after centrifugation, take the precipitated bacterial cells and add a small amount of PBS solution to prepare a bacterial suspension. According to the experimental results of Example 2, a certain amount of bacterial suspension was inoculated ...

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Abstract

The invention relates to an aeromonas cainea W3 capable of degrading typical fluoroquinolone antibiotics and application thereof. The collection number of the guinea pig aeromonas sp. W3 is CCTCC M 2021633, and the guinea pig aeromonas sp. W3 is collected in China Center for Type Culture Collection on May 31, 2021. The Aeromonas cavieae W3 is gram-negative bacteria, the bacterial colony on an MSM plate is round, the surface is smooth, wet and easy to pick up, and the color is transparent to white. The Aeromonas cavieae W3 provided by the invention has relatively strong degradation capability on typical fluoroquinolone antibiotics under an aerobic condition, is suitable for treating pollution of the typical fluoroquinolone antibiotics in an environment, and can achieve the purpose of environmental remediation.

Description

technical field [0001] The invention belongs to the technical field of Aeromonas caviae. More specifically, it relates to an Aeromonas caviae W3 that degrades typical fluoroquinolone antibiotics and its application. Background technique [0002] Norfloxacin (NOR) and Ciprofloxacin (Ciprofloxacin, CIP) are third-generation quinolone antibiotics, commonly used in clinics and livestock and poultry farming, and enter the environment mainly through household wastewater, hospital wastewater and breeding wastewater, urban Quinolones antibiotics cannot be completely removed by WWTPs and still exist in sewage and sludge from WWTPs and can be transferred to surface water and groundwater, negatively impacting non-target organisms, leading to the disappearance of certain microbial populations and their ecological functions , and even accumulate through the food chain to cause health risks to the human body. In addition, antibiotic residues can promote the evolution and transfer of ant...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC12N1/20C02F3/34C02F2101/38Y02W10/10
Inventor 彭星星黄梓丹
Owner SUN YAT SEN UNIV
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