Aeromonas algicide and application thereof to removal of cyanobacterial bloom

A technology of Aeromonas and cyanobacteria blooms, applied in herbicides and algaecides, application, sterilization/microdynamic water/sewage treatment, etc., can solve the secondary pollution of water, high cost and adverse effects on the ecological environment and other problems, to achieve the effects of simple preparation process, safe product use, and stable algae removal activity.

Active Publication Date: 2013-07-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method is relatively simple and can be operated on a large area, but most algaecides are not effective in removing algae, and have adverse effects on the ecological environment, and the cost is high
Due to the high cost of water treatment and the easy production of toxic by-products leading to secondary pollution of water bodies in physical and chemical algae removal methods, their widespread use in practical applications is limited. In recent years, environmentally friendly biological algae control methods have received more and more attention. more and more attention

Method used

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  • Aeromonas algicide and application thereof to removal of cyanobacterial bloom
  • Aeromonas algicide and application thereof to removal of cyanobacterial bloom
  • Aeromonas algicide and application thereof to removal of cyanobacterial bloom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Inoculate 1 loop of a single colony of Aeromonas sp.DS-1CCTCC No: M2011180 into 50mL beef extract peptone liquid medium, shake and culture at 180rpm / min at 30°C for 36 hours, and inoculate the obtained bacterial solution with 6wt % inoculum size was inserted into 700mL beef extract peptone liquid medium, and shaken and cultivated for 36 hours under 180rpm / min rotating speed under the condition of 30°C to obtain seed liquid; Beef extract peptone liquid medium, the medium is 70% of the volume of the fermenter. For fermentation culture, the sterilization parameters were 121°C for 20 minutes; the fermentation parameters were pH 6.5, DO 80, fermentation temperature 30.0°C, stirring speed 120 rpm, and fermentation time 36 hours. The fermentation product was centrifuged at 8000rpm / min for 5min to remove bacterial cells, the supernatant was extracted twice with n-butanol, and the n-butanol was removed by rotary evaporation to obtain a solid preparation.

[0031] The chromatogram...

Embodiment 2

[0036] Inoculate 2 rings of single colonies of Aeromonas sp.DS-1CCTCC No: M2011180 into 60mL beef extract peptone liquid medium, shake and culture at 180rpm / min at 30°C for 38 hours, and inoculate the obtained bacterial solution with 8wt % inoculum size was inserted into 900mL beef extract peptone liquid medium, and under the condition of 30°C, 180rpm / min was shaken and cultivated for 38 hours to obtain seed liquid; Beef extract peptone liquid medium, Aeromonas fermentum sp.DS-1, sterilization parameters are 121°C, 20 minutes; fermentation parameters are, pH6.5, DO is 80, fermentation temperature is 30.0°C, stirring speed is 120 rpm / minute, fermentation time 36 hours. Centrifuge the fermentation product at 8000rpm / min for 5min to remove bacterial cells, extract the supernatant twice with recovered n-butanol, remove n-butanol by rotary evaporation to obtain a solid preparation, collect n-butanol for reuse without affecting the extraction efficiency and preparation results. ...

Embodiment 3

[0041] Take 1 L of Microcystis aeruginosa FACHB927 culture solution in the adaptation stage and add them to Reactor A and Reactor B respectively. The algae density is 1×10 6 Cell / L, wherein 0.002 g of the algicide of Example 1 was added to reactor A, and no algicide was added to reactor B as a control. After 2 days of treatment, water samples were taken from reactor A and reactor B to measure the number of algae cells. The algae removal rate of the water samples in reactor A was above 95%, and the algae density in control group B increased.

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Abstract

The invention relates to an aeromonas algicide and application thereof to the removal of cyanobacterial bloom. The aeromonas algicide is a solid state preparation prepared by extracting fermentation liquor of aeromonas sp.DS-1 through n-butyl alcohol and evaporating to remove the n-butyl alcohol, wherein the collection number of the used aeromonas sp.DS-1 is CCTCC No. M2011180, and the collectionunit is China Center for Type Culture Collection. The aeromonas algicide is used for eliminating the cyanobacterial bloom, the using dose of the algicide is between 2 and 300 mg / L, and the cyanobacterial bloom containing 10<5> to 10<9> cells / L can be removed effectively.

Description

technical field [0001] The invention relates to an algaecide prepared by Aeromonas fermentation and its application for removing algal blooms, and belongs to the technical field of biological environmental protection. Background technique [0002] At present, many fresh water bodies in our country are in a severe eutrophication situation. The frequent occurrence of algae blooms seriously affects the water quality of the water body. It will greatly increase the difficulty of drinking water treatment for source water, and at the same time threaten human health, thereby generating huge economic benefits. loss and social harm. The physical method of water bloom control is to use certain equipment and equipment to set up a specific safety isolation area in the water body, separate the bloom algae in the water body of the water bloom, or use a mechanical device to remove algae, such as direct filtration, air flotation, etc. Algae, enclosure fence, ultrasonic method, light isolati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P1/00A01P13/00C02F1/50C12P1/04C12R1/01
Inventor 李力刘玉梅
Owner SHANDONG UNIV
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