Algae-lysing aeromonas sp. and application thereof in controlling cyanobacterial blooms

A technology of Aeromonas and cyanobacteria blooms, which is applied in the field of environmental microorganisms, can solve the problems of secondary pollution and limited processing capacity, and achieves the effects of simple preparation method, short preparation period and good algae-dissolving effect.

Inactive Publication Date: 2016-09-21
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the limited processing capacity of physical and chemical methods in the prior art, and the defects of easily causing secondary pollution, the technical problem to be solved in the present invention is to find efficient algae-dissolving bacteria and to separate and enrich the highly efficient algae-dissolving active substances produced by the metabolism of algae-dissolving bacteria , for safe and efficient control of cyanobacterial blooms

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  • Algae-lysing aeromonas sp. and application thereof in controlling cyanobacterial blooms
  • Algae-lysing aeromonas sp. and application thereof in controlling cyanobacterial blooms
  • Algae-lysing aeromonas sp. and application thereof in controlling cyanobacterial blooms

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Embodiment 1

[0033] The screening of embodiment 1 algae-lytic bacteria

[0034]Add 5mL of natural water samples collected from the Meiliang Bay waters of Taihu Lake to 95mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase. After the algae liquid is yellowed, it is serially diluted and coated with beef extract peptone medium agar plate, cultured at 28°C for 24 hours, and a plate with a moderate colony density is taken, and different strains are selected according to the different colony forms.

[0035] The screened strains were inoculated into 8 mL of beef extract peptone medium, cultured at 28°C and 220 rpm for 24 hours, and 1 mL of cultured bacterial liquid was added to 99 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 1 mL of sterilized beef extract peptone medium was also added to 99 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator for 6 days...

Embodiment 2

[0039] Embodiment 2 Identification of Aeromonas GLY-2107 bacterial strain

[0040] GLY-2107, which has a strong algicidal effect, was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. μm~1.2μm×2.0μm~2.5μm. After being cultured on the nutrient agar solid medium plate for 24 hours, the colony shape is round, the surface is smooth and flat, the edges are neat, white, and the colony size is 2mm to 3mm. After 16S rRNA gene sequence analysis and homology comparison, it was known that it had 99% homology with a certain Aeromonas strain in GenBank, so it was identified as Aeromonas bacteria and named Aeromonas GLY- 2107. The strain has been preserved in the General Microbiology Center of the National Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.8979, and the preservation date is March 28, 2014. Address of depository institution: Institute of Microbiology, Ch...

Embodiment 3

[0041] The preparation method of embodiment 3 Aeromonas GLY-2107 fermented liquid and ethyl acetate extract thereof

[0042] Aeromonas GLY-2107 was inoculated in sterilized beef extract peptone medium according to 1% inoculation amount, and cultured on a shaker at 220 rpm at 28° C. for 48 hours to obtain Aeromonas GLY-2107 fermentation broth. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

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Abstract

The invention discloses algae-lysing aeromonas sp. and an application thereof in controlling cyanobacterial blooms. The aeromonas sp. GLY-2107, which has a significant algae-lysing activity, is separated from a water body from Lake Taihu, and the preservation number of the aeromonas sp. is CGMCC No.8979; and active algae-lysing ingredients, namely 3-phenmethyl-piperazine-2,5-dione and 3-methylindole, are separated, purified and identified from a metabolic product of the aeromonas sp., wherein the median lethal dose LD50 of the 3-phenmethyl-piperazine-2,5-dione on microcystis aeruginosa 9110 is 4.72 [mu]g / mL and the median lethal dose LD50 of the 3-methylindole on the microcystis aeruginosa 9110 is 1.10 [mu]g / mL. The algae-lysing aeromonas sp. is applicable to research, development and production of novel biological algicides, and is finally applied to the control of the cyanobacterial blooms in lakes.

Description

technical field [0001] The invention relates to the field of environmental microbes, in particular to a strain of Aeromonas sp. GLY-2107 with algae-dissolving activity and its application in the control of cyanobacteria blooms. Background technique [0002] In recent years, Taihu Lake, Chaohu Lake, Dianchi Lake and other freshwater waters have been seriously affected by cyanobacteria blooms, and the cyanobacteria blooms caused by lake eutrophication have increasingly polluted lake water systems. Therefore, it is very necessary to explore effective ways to control the cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] The control technology of cyanobacteria bloom can be summarized as physical method, chemical method and biological method. Physical methods, such as mechanical algae removal, electromagnetic field algae removal, etc., can be used as auxiliary control measures for algae blooms, but the disadvantages are that they need a lot of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N43/60A01N43/38A01P13/00C02F3/34C02F1/50C12R1/01
CPCA01N43/38A01N43/60A01N63/10C02F1/50C02F3/34C12N1/205C12R2001/01A01N2300/00
Inventor 杨虹郭星亮
Owner SHANGHAI JIAO TONG UNIV
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