Blue-green alga gene transfer method favorable to harvest

Abstract

The invention discloses a gene transfer method for cyanobacteria that is beneficial to harvesting, and relates to a gene transfer method for microorganisms, in particular to a gene transfer method for introducing an exogenous gene into a filamentous nitrogen-fixing cyanobacteria capable of agglomerating growth. In order to overcome the non-caking problem of cyanobacteria currently used to express foreign genes, a filamentous nitrogen-fixing cyanobacteria that grows in caking and floating growth was screened from nearly 70 algae strains to establish a gene transfer system, so that the algae can be obtained from Fishing directly from the culture solution or harvesting by filtering with ordinary nylon mesh is beneficial to the industrialization of cyanobacteria genetic engineering. In order to adapt to the gene transfer of this kind of cyanobacteria, important improvements have been made to the combination mode of cyanobacteria.

Description

technical field [0001] The invention relates to a gene transfer method for microorganisms, in particular to a gene transfer method for introducing an exogenous gene into a filamentous nitrogen-fixing cyanobacteria capable of agglomerating growth. technical background [0002] Genetic engineering can be used to express valuable genes in microorganisms, animals and plants to produce genetically encoded products, or to modify the traits of microorganisms, animals and plants. Commonly used microorganisms include Escherichia coli, Bacillus, and yeast. The gene transfer and expression systems of these microorganisms have been matured after years of development, and have been successfully used in the production of insulin, interferon, interleukin and other drugs. It has the advantages of high expression, perfect protein extraction and purification methods, etc.; but the high cost of cultivation can only be applied to the production of expensive drugs. If it is used in the field o...

AI Technical Summary

Problems solved by technology

They grow uniformly and dispersed, which is convenient for counting and quantification, and is suitable for research work, but it is difficult to carry out large-scale production in an open system, and the cells or filaments are small and cannot be collected by filtration, even if they are produced, they are not easy to collect algae cell

These difficulties have become a bottleneck restricting the industrialization of cyanobacteria genetic engineering technology. Therefore, if a gene transfer method can be established in cyanobacteria that can grow in agglomerates and float, it will be a breakthrough in cyanobacteria genetic engineering.