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Integration processing method of high-efficient corn straw biological feed

A technology of corn stalks and processing methods, applied in animal feed, animal feed, food processing, etc., can solve the problems of slow growth of white rot fungus Trichoderma, easy to infect bacteria, long time, etc.

Inactive Publication Date: 2016-09-07
陈华友
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to improve the degradation of lignin, some relatively scientific invention patents carry out co-fermentation of fiber degrading bacteria and white rot fungi, such as (103146606B) and (103549129B), the former uses Phanerochaete chrysosporium, Trichoderma, yeast, Bacillus subtilis Co-fermentation with lactic acid bacteria, straw aerobic fermentation and fermentation with anaerobic bacteria, but the slow growth of white rot fungus and the fast growth of Trichoderma lead to long aerobic fermentation time, high cost and easy to infect bacteria
The latter also uses the slow-growing Phanerochaete xanthophyllum, and co-fermentation with the fast-growing Trichoderma, Penicillium, and Aspergillus will cause inconsistencies, and the fermentation time will be long. The peak of lignin enzyme production is generally after 7 days, and the fermentation time also needs to be longer. After 10 days, the degradation of lignin is not significant under compaction and sealing, and the degradation rate is generally less than 20%.

Method used

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  • Integration processing method of high-efficient corn straw biological feed
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  • Integration processing method of high-efficient corn straw biological feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Pre-production of white rot fungus enzyme preparation

[0071] (1) White rot fungus culture medium.

[0072] Slant medium and plate medium: 200g potato, 20g sucrose, 20g agar, in 1L constant volume, pH 5.0, sterilized at 110°C for 30min. Inoculate and culture at 37°C for 4 days.

[0073] Seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort juice or glucose 3g, ammonium tartrate 1g, dilute to 1L, in which FeSO 4 ·7H 2 O, ZnSO 4 、CuSO 4 ·5H 2 O, MnSO 4 .4H 2 O and CoCl 2 Each 0.001% (weight ratio), pH 5.5. Add 100ml medium to a 500ml Erlenmeyer flask, and sterilize at 110°C for 30min.

[0074] White-rot fungus solid-state fermentation seed culture medium: by weight, cut, shredded, and crushed corn stalks (below 20mm) account for 80% of the culture medium (calculated as dry material), 15% of bran, ammonium tartrate, and ammonium nitrate , ammonium sulfate or ammonium chloride accounted for 0.3%, CaCO 3 1%, KH 2 P...

Embodiment 2

[0078] The first phase of corn straw fermentation

[0079] (1) Seed medium and production medium of fiber-degrading bacteria such as Neurospora

[0080] Neurospora and other slant medium and plate medium: 200g potato, 20g sucrose, 20g agar, constant volume 1L, pH 5.0, sterilized at 110°C for 30min. Inoculate and culture at 32°C for 2 days.

[0081] Neurospora and other seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort juice or brown sugar 3g, ammonium sulfate or urea 5g, FeSO 4 ·7H 2 O 0.005g, ZnSO 4 0.0014g, MnSO 4 4H 2 O 0.0016g, CoCl 2 0.002g, dilute to 1L, pH 5.0. Add 100ml medium to a 500ml Erlenmeyer flask, and sterilize at 110°C for 30min.

[0082] Candida utilis slant culture medium and plate culture medium (100ml): glucose 2g, yeast extract 1g, peptone 1g, urea 1g, agar 2g, pH value about 6.0.

[0083] Candida utilis liquid medium: the composition is the same as that of the slant medium, without adding agar. ...

Embodiment 3

[0091] The second phase of corn straw fermentation

[0092] (1) Bacillus culture medium

[0093] Bacillus slant and plate culture medium: peptone 10g, beef extract powder 5g, sodium chloride 5g, agar 15g, glucose 20g, distilled water 1000ml, final pH7.0±0.2. Sterilize at 121°C for 20min.

[0094] Bacillus shake flask seed medium and seed tank medium: beef extract 5.0g / L, peptone 20.0g / L, glucose 5.0g / L, FeCl 2 ·6H 2 O 0.07g / L, MnCl 2 ·7H 2 O 0.01g / L, MgSO 4 ·7H 2 O 0.15g / L, pH 6.5-7.0, sterilized at 110-121°C for 20min.

[0095] (2) Saccharomyces cerevisiae medium

[0096] Yeast slant medium and plate culture medium (100ml): 2g glucose, 1g yeast extract, 2g peptone, 2g agar, pH value about 6.0.

[0097] Shake flask seed medium and liquid seed tank medium: the composition is the same as that of the slant medium, without adding agar.

[0098] (3) The second-stage corn stalk solid-state fermentation medium is composed of the first-stage fermentation product plus 5% corn ...

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Abstract

The invention discloses an integration processing method of a high-efficient corn straw biological feed, and belongs to the field of biological feeds. Corn straw is subjected to three-step fermentation, so that a superior microorganism coarse feed for ruminant animals, with high efficiency and low cost, is produced in a large-scale manner. The integration processing method comprises the following steps of firstly, based on the corn straw, performing high-efficient pure solid cultivation on a white rot fungus enzyme preparation, then converting the cultivated white rot fungus enzyme preparation in large inoculum amount to a first-stage fermentation medium of the corn straw, and besides, inoculating serial lignocelluloses degradation bacteria and candida utilis bacteria for synergistic rapid degradation and transformation; then adding bacillus and saccharomyces cerevisiae for second-stage fermentation; and using lactic acid bacteria for third-stage anaerobic fermentation so as to obtain finished products. The raw materials used by the integration processing method do not need to be sterilized, the finished products do not need to be dried, lysozymes are enriched, the cost is low, the lignin is notably reduced, and genuine protein is increased to 12% or above. The high-efficient corn straw biological feed can be used as a superior coarse feed and a protein grain feed for cattle and sheep, and the additive quantity can reach 60% or above; the additive quantity can also be about 10%, so that the high-efficient corn straw biological feed is used for simple stomach animals. The technique is high in scalability and integrity, and the economic and social benefits are notable.

Description

technical field [0001] The invention belongs to the field of biological feed, in particular to a novel microbial roughage with high efficiency and low cost based on corn yellow mature stalks as raw materials. Background technique [0002] Corn stalks are one of the most abundant straws in China. If they are used as energy-based materials such as power generation, biogas, gasification, and solidification molding, the economic benefits will be low. Straw ethanol, whose production cost is more than 7,000 yuan per ton, is not easy to produce without state subsidies. Profit; fertilization is actually a huge waste of resources; the total amount used for processing such as board is limited; compared with rice straw, wheat straw, etc., corn straw has better nutritional indicators, and the purchase price in many places is twice as low as that of wheat straw , corn stalks are a good way out for feed, but most of the harvested corn stalks are not processed and fed directly, which has d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K20/158A23K20/26A23K10/14
CPCY02P60/87
Inventor 陈华友吴邦国杨胜利谢永明
Owner 陈华友
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