Microbial strain and its application in degumming of cocoon silk
A technology of microorganisms and cocoons, applied in microbial strains and its application fields, can solve the problems of long production cycle, high cost of enzyme refining, poor working environment, etc., and achieve the effect of environmental protection in the production process, simple operation method and mild effect
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Embodiment 1
[0025] A microbial strain that can be used for cocoon silk degumming is Aeromonas, and its Latin name is: Aeromonassp.TJ-5. CCTCC NO: M2017192.
Embodiment 2
[0027] The screening method of microbial strain Aeromonas sp.TJ-5, comprises the following steps:
[0028] (1) Add 10mL of cocoon silk decomposing liquid obtained by sampling into 90mL of seed medium prepared as follows: 10g / L tryptone, 5g / L yeast extract, and 5g / L NaCl, pH 7.0; then placed on a shaker at 37°C, shaken at 180rpm for 12 hours to obtain a bacterial suspension;
[0029] (3) Dilute the above-mentioned cultured bacterial suspension to different times and spread it on the seed liquid agar plate, and cultivate it at 37°C for 24 hours;
[0030] (4) Pick a single colony and inoculate it into the degumming medium. The degumming medium is prepared as follows: 3g / L ammonium nitrate; 0.5g / L potassium dihydrogen phosphate; 1.5g / L disodium hydrogen phosphate, 1g / LNaCl, 0.2g / L magnesium sulfate heptahydrate; pH 7.0, then add 0.5g cocoon silk, then shake and culture at 37°C and 180rpm for 48h.
[0031] (5) Measure and calculate cocoon degumming rate.
[0032] After the degum...
Embodiment 3
[0038] Microbial strain Aeromonas Aeromonassp.TJ-5 can be applied to remove sericin in cocoon silk. The specific cocoon silk degumming application includes the following steps:
[0039] (1) preparation of degumming microorganisms;
[0040] (2) cocoon silk microbial degumming treatment;
[0041] (3) Water washing: after the degumming is finished, take out the cocoon silk and wash it with water;
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