Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial strain and its application in degumming of cocoon silk

A technology of microorganisms and cocoons, applied in microbial strains and its application fields, can solve the problems of long production cycle, high cost of enzyme refining, poor working environment, etc., and achieve the effect of environmental protection in the production process, simple operation method and mild effect

Active Publication Date: 2017-11-17
四川省丝绸工程技术研究中心 +1
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is characterized by high chemical refining efficiency and low production cost, but due to the use of a large amount of alkali and other chemical raw materials, the fiber damage is greater and the environment is seriously polluted; corrosion refining is generally carried out through chemical refining first, and then the raw materials are treated with microbial soaking liquid. Corrosion (fermentation) treatment is carried out to remove part of the sericin and grease, and then the raw materials are processed to meet the process requirements by chemical refining (re-training)
Corrosion refining has a good degreasing effect, relatively speaking, the amount of chemical materials used is less than the former, but this process has problems such as long production cycle, unstable product quality, environmental pollution caused by corrosion odor, and poor working environment.
In recent years, enzymatic degumming has also been used, which has the advantages of mild action and environmental protection, but the cost of enzyme refining is high, and there are few types of enzyme preparations, so it has not been widely used yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial strain and its application in degumming of cocoon silk
  • Microbial strain and its application in degumming of cocoon silk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A microbial strain that can be used for cocoon silk degumming is Aeromonas, and its Latin name is: Aeromonassp.TJ-5. CCTCC NO: M2017192.

Embodiment 2

[0027] The screening method of microbial strain Aeromonas sp.TJ-5, comprises the following steps:

[0028] (1) Add 10mL of cocoon silk decomposing liquid obtained by sampling into 90mL of seed medium prepared as follows: 10g / L tryptone, 5g / L yeast extract, and 5g / L NaCl, pH 7.0; then placed on a shaker at 37°C, shaken at 180rpm for 12 hours to obtain a bacterial suspension;

[0029] (3) Dilute the above-mentioned cultured bacterial suspension to different times and spread it on the seed liquid agar plate, and cultivate it at 37°C for 24 hours;

[0030] (4) Pick a single colony and inoculate it into the degumming medium. The degumming medium is prepared as follows: 3g / L ammonium nitrate; 0.5g / L potassium dihydrogen phosphate; 1.5g / L disodium hydrogen phosphate, 1g / LNaCl, 0.2g / L magnesium sulfate heptahydrate; pH 7.0, then add 0.5g cocoon silk, then shake and culture at 37°C and 180rpm for 48h.

[0031] (5) Measure and calculate cocoon degumming rate.

[0032] After the degum...

Embodiment 3

[0038] Microbial strain Aeromonas Aeromonassp.TJ-5 can be applied to remove sericin in cocoon silk. The specific cocoon silk degumming application includes the following steps:

[0039] (1) preparation of degumming microorganisms;

[0040] (2) cocoon silk microbial degumming treatment;

[0041] (3) Water washing: after the degumming is finished, take out the cocoon silk and wash it with water;

[0042] (4) Drying: drying the washed cocoons.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a microbial aeromonas strain which can be used for degumming of cocoon silk. The Latin name of the strain is Aeromonas sp.TJ-5, the strain is preserved in the China center for type culture collection on April 14 in 2017, the preservation number is CCTCC NO:M2017192, and the strain is screened from cocoon silk corrosive liquid. The invention further provides an application method that the strain is directly applied to raw cocoon silk materials to effectively remove sericin in the cocoon silk. Through degumming microorganism preparation, cocoon silk degumming by using degumming microorganisms, water washing and drying steps, sericin in the cocoon silk is effectively removed, the use of chemical materials in the degumming process of silk spinning raw materials can be further reduced, the working environment is improved, and the environment pollution is reduced.

Description

technical field [0001] The invention relates to a microbial strain and its application field, in particular to an Aeromonas strain and the application of the strain in cocoon silk degumming. Background technique [0002] China is the world's largest silk country, with an annual silk production of about 100,000-150,000 tons. Usually, every ton of raw silk produced will have about 14-20% cocoon silk wastes such as defective cocoons, waste silk, stagnant ends, reeling residues, etc. to become raw materials for silk spinning. In addition to sericin and grease, these leftovers also have other pollutants and impurities attached to them. Most of the sericin, grease, wax, etc. must be removed through refining and finishing. [0003] At present, the scouring method of silk spinning raw materials commonly used in China is the chemical method or the scouring method combined with chemical-corrosion method. It is characterized by high chemical refining efficiency and low production cos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20D01C3/02C12R1/01
CPCC12N1/20C12N1/205C12R2001/01D01C3/02
Inventor 吴重德方佳周荣清李乔兰肖露张勇杨旭超
Owner 四川省丝绸工程技术研究中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products