The invention discloses a serum protein mass spectrum model for screening pancreatic cancer and a construction method thereof. The serum protein mass spectrum model mainly comprises human serum proteins, the cytoplasm-nuclear ratios (M / Z) of which are positioned at 4,976+ / -380Da, 6,857+ / -737Da, 4,407+ / -726Da, 5,488+ / -963Da, 7,068+ / -142Da, 3,250+ / -591Da, 4,753+ / -360Da, 6,885+ / -572Da, 5,644+ / -382Da, 2,892+ / -299Da, 3,281+ / -348Da, 4,292+ / -289Da, 2,678+ / -208Da and 5,364+ / -126Da in human serum protein mass spectrums. The construction method is reasonable, feasible and simple and convenient in operation, and can be used for batch processing.