The invention provides a preparation method of a sterilization medicament. The preparation method of the sterilization medicament is characterized in that liquorice, radix scutellariae, dried
alum and
honeysuckle are smashed, the mixture is screened with a
sieve with the mesh numbers being 20-40, ethyl
alcohol with the concentrate being 30%-70% is added to the smashed mixture to completely submerge the smashed mixture, the amount of the ethyl
alcohol is 6-7 times that of the smashed mixture, and dipping is conducted for 24-72 h; then, the mixture is percolated in a percolator, large-sized particles are filtered off, obtained filter liquor is heated, residual ethyl
alcohol is volatilized, and the obtained liquor is concentrated until the
solid-to-
liquid ratio is 1:1;
centrifugal separation or resin adsorption is conducted, fine particles and other impurities are filtered off, and obtained filter liquor stands for use;
iodine is mixed with an emulsifying agent, and a cream object is obtained through emulsification and complexation and stands for use; the filter liquor and the cream object are mixed and evenly stirred, water is added to the mixture for attenuation, and after even stirring, medical
phosphoric acid is added to adjust the ph to be 1.3-2.8; and finally, the product is obtained after filling and sealing.