30results about How to "Simple disinfection method" patented technology

The present invention discloses a disinfection method of bamboo embryo culturing, which takes healthy seed of bamboo in the same year as an explant, soaks the grain after shelling in medical alcohol for 4-6 min, and then stirs it in 0.1 wt. % mercuric chloride solution for 25-35 min or in 0. 2-0.3 wt. % sodium hypochlorite solution for 10-20min, at last drops it in medical alcohol for 4-6 min. The disinfection method is simple and can effectively remove surface bacilli and endogenetic bacilli, which lay the foundation of establishing sterilized system, promoting successive transfer culture and rooting, and provides technical characteristics for superior bamboo seed development and utilization.

The invention discloses a method for quickly reproducing miscanthus plant miscanthus and triarrhena hybrid NO.9 somatic embryo tissue culture, which comprises the following steps of: (1) selecting young ears: selecting young ears of miscanthus and triarrhena hybrid NO.9 staying in the gramineae primordium formation period; (2) inducing the young ears by using a primary induction culture medium: cutting the young ears into small segments, disinfecting the segments with alcohol, soaking the segments in mercuric chloride, flushing the segments with sterile water, inoculating the segments on the primary induction culture medium of young ears, and inducing primary generation to obtain embryonal calluses; (3) cubculturing by using a seedling culture medium: subculturing the obtained embryonal calluses on a subculture medium; (4) inducing to grow roots by using a root growing induction culture medium: inducing the grown seedlings on the induction culture medium to grow roots; and (5) domesticating and transplanting: spraying a domesticating culture liquid and clean water to the seedlings regularly every day in a greenhouse, transplanting the seedlings to a nursery, and illuminating the seedlings to obtain the seedlings of miscanthus and triarrhena hybrid NO.9. The method is feasible and simple and convenient to operate, the differentiation callus induction rate and the qualified seedling percentage are high, the survival rate is high, and the method is applicable to quick reproduction in the industrial production of miscanthus and triarrhena hybrid NO.9 seedlings.

The invention discloses a method for tissue culture and rapid propagation of weeping tube flowers, which comprises the following steps: setting a culture medium; selecting and disinfecting explants to ensure that there are no defects in the outer scales; putting the scale buds after the disinfection treatment on the induction medium Carry out the induction culture of adventitious buds; the induced adventitious bud clusters are multiplied by adventitious buds, and the multiplied adventitious bud clusters are divided into individual plants under aseptic conditions and then transferred to the rooting medium for rooting induction culture; After the induction of rooting, the weeping tube flower plants are transplanted and hardened. The invention can obtain a large amount of high-quality and strong seedlings of the weeping tube flower with the same genetic character in a short period of time.

The invention discloses a culture method of peony embryonic callus as well as a culture medium. The peony embryonic callus is induced through adopting an MS culture medium which contains special components after modification (such as substances of 6-BA, KT cytokinin, TDZ plant growth regulators, PIC herbicide as well as casein hydrolysate); aiming at a peony embryo explant, the induction rate of the peony callus can achieve 100% by adopting the specific culture method provided by the invention, wherein the induction rate of embryonic callus can achieve 86% above.

The invention relates to a new method for disinfecting an accellular protein scaffold. The method comprises the steps of: disinfecting a protein scaffold with a low-concentration peracetic acid neutralized with 10% sodium hydroxide in combination with a hypertonic ion solution, thereby achieving the purpose of killing bacteria and spores in the collagen scaffold without denaturing the protein scaffold and also facilitating the cell growth during late recellularization. The method has the advantages that the new disinfection method is simple and easy to operate, has high disinfection efficiency, can reduce the influence on the physical properties of the accellular protein scaffold and reduce the edema caused by long-time soaking of the protein scaffold, and is beneficial to the late recellularization process.

The invention discloses a rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant, comprising the following steps: A, selecting young ear: selecting the yound ear at Ying flower primordium formation period; B, carrying out primary induction with a primary culture medium: cutting to small sections, sterilizing with alcohol, soaking with mercuric chloride,washing and inoculating on the primary induction culture medium; C, subculturing seedling with a seedling culture medium: subculturing and generating the seedling on a subculture culture medium with the obtained embryonic callus, subculturing, multiplication and shining every day; D, carrying out radication induction with a radication induction culture medium; carrying out the induction radication on the radication induction culture medium by seedling, culturing and shining every day; E, acclimating and transplanting: transplanting the tissue culture seeding to an acclimation nursery garden consisting of vermiculite to acclimate, spraying acclimation culture solution and clean liquid to the seeding at fixed time every day, transplanting to the nursery garden and obtaining the miscanthus floridulu seeding. The method is easy to apply, simple to operate, and large in breed coefficient; and the recovery reproduction rate is high, which is about 100%.

The invention discloses a culture medium and a culture method suitable for anthers and ovaries of weeping flax, belonging to the field of wild flax biotechnology. The culture method includes genotype selection, explant low temperature treatment, explant surface disinfection, callus induction, callus differentiation, adventitious bud elongation and rooting culture. The present invention uses the anthers and unfertilized ovaries of flax as materials to establish a haploid regeneration plant culture system suitable for flax and directly obtains haploid plants of flax, which are haploid of flax Sports breeding provides a certain reference value. At the same time, it has certain significance for the preservation of flax genetically improved germplasm resources and the selection of new varieties. It is also a means of preserving wild genetic resources and providing assistance for future scientific research.

The invention discloses a method for breeding cherry seedlings in large scales through tissue culturing. The method comprises the following steps of: (1) pretreatment of to-be inoculated materials; (2) disinfection of explants; (3) establishment of sterile systems; (4) multiplication culturing of buds; (5) strong seedling and rooting culturing; and (6) transplantation of test-tube seedlings. The method for rapidly breeding cherry seedlings through tissue culturing has the advantages of a low contamination rate of explants, a high breeding coefficient, good seedling quality and a high transplanting survival rate; according to the method, the defects of a low breeding coefficient, a long period and season limitation of the conventional technology are overcome.

The invention discloses a tissue culture and rapid propagation method of nobile-type dendrobium seedlings. The method comprises the following steps of: (1) selection and disinfection of an explant; (2) induction culture of protocorm; (3) proliferation and differentiation of protocorm; (4) strong seedling and rooting culture; (5) transplanting of test-tube plantlets. According to the method, tissue culture and rapid propagation of the nobile-type dendrobium seedlings are carried out by the method provided by the invention, the culture procedure is simple, the explant is free of pollution, the propagation coefficient is large, the production cost is low, the seedlings are high in quality, and the defects of low propagation coefficient and the like in the prior art are overcome.

The invention discloses an efficient expanding propagation method for dahlia toxin-free seedlings. The method includes the following steps of 1, pretreatment of dahlia toxin-free seedlings, 2, induction of adventitious buds, 3, cutting of adventitious buds for mulitiplication, 4, hardening-seedling, 5, water planting and rooting, 6, water planting of strong seedlings and 7 transplanting. The efficient expanding propagation method solves the problems that dahlia toxin-free seedlings are low in efficiency in the expanding propagation process and prone to secondary pollution. A great number of excellent dahlia toxin-free seedlings can be cultured in an expanding propagation mode in a short period of time, the problem of variety degeneration brought by dahlia division propagation and seed propagation can be avoided, and the method is easy to operate and has higher practical value in production, and industrialized popularization is achieved easily.

The invention relates to a method for cultivating seedlings of a gerbera group. The method for cultivating seedlings of a gerbera group comprises the following steps: (1) selection and treatment of explants; (2) induction of adventitious buds; (3) proliferation of adventitious buds ; (4) rooting of adventitious buds; (5) seedling hardening and domestication. The invention has good disinfection effect, short rooting time, fast growth time and high seedling quality.

The invention discloses a method for rapidly breeding miscanthus plant miscanthus giganteus embryo by tissue culture, comprising the following steps: A, selecting a young ear; B, carrying out primary induction, namely cutting the young ear into sections, sterilizing with alcohol, soaking with mercury chloride, then washing with sterile water, and inoculating the sections onto a primary induction culture medium of the young ear; C, carrying out subculture for seedling growth, namely carrying out successive transfer seedling growing on the obtained embryonic callus onto a subculture medium, carrying out subculture once, and transferring the embryonic callus onto a seedling growing culture medium, thus small young seedling is formed; D, carrying out root generating induction, namely inducting the young seedling on a root growing induction culture medium to generate a root, culturing, and transferring the young seedling onto a root growing culture medium, thus the young seedling differentiates white or yellow roots; and E, carrying out acclimatization and transplanting, namely transplanting the seedling into a flower pot filled with vermiculite and soil to be acclimatized, spraying acclimatization culture liquid and clear water onto the young seedling regularly, and transferring the seedling into a seedling nursery. The method is feasible and is simple to operate, the rapid breeding efficiency and the breeding coefficient of miscanthus giganteus are improved in short time, the appreciation is stable, the root system is robust, and the transplanting survival rate is high.

The invention discloses a high-efficiency multiplication method for virus-free gerbera seedlings, which comprises the following steps: 1) pretreatment of gerbera chrysanthemum virus-free seedlings; 2) induction of adventitious buds; 3) cutting adventitious buds for subculture; 4) Seedling hardening; 5) Hydroponic rooting treatment; 6) Hydroponic strong seedlings; 7) Transplanting. The high-efficiency multiplication method of the present invention solves the problems of low efficiency and easy re-pollution during the multiplication process of the virus-free gerbera seedlings. The problem of variety degeneration caused by chrysanthemum branch propagation and seed propagation is simple, easy to promote in factories, and has high practical value in production.