Method for quickly reproducing miscanthus plant miscanthus and triarrhena hybrid NO.9 somatic embryo by tissue culture

Abstract

The invention discloses a method for quickly reproducing miscanthus plant miscanthus and triarrhena hybrid NO.9 somatic embryo tissue culture, which comprises the following steps of: (1) selecting young ears: selecting young ears of miscanthus and triarrhena hybrid NO.9 staying in the gramineae primordium formation period; (2) inducing the young ears by using a primary induction culture medium: cutting the young ears into small segments, disinfecting the segments with alcohol, soaking the segments in mercuric chloride, flushing the segments with sterile water, inoculating the segments on the primary induction culture medium of young ears, and inducing primary generation to obtain embryonal calluses; (3) cubculturing by using a seedling culture medium: subculturing the obtained embryonal calluses on a subculture medium; (4) inducing to grow roots by using a root growing induction culture medium: inducing the grown seedlings on the induction culture medium to grow roots; and (5) domesticating and transplanting: spraying a domesticating culture liquid and clean water to the seedlings regularly every day in a greenhouse, transplanting the seedlings to a nursery, and illuminating the seedlings to obtain the seedlings of miscanthus and triarrhena hybrid NO.9. The method is feasible and simple and convenient to operate, the differentiation callus induction rate and the qualified seedling percentage are high, the survival rate is high, and the method is applicable to quick reproduction in the industrial production of miscanthus and triarrhena hybrid NO.9 seedlings.

Description

technical field [0001] The present invention relates to the technical field of tissue culture rapid propagation based on embryogenic callus of Miscanthus hybrid No. 9, and more specifically relates to a method for rapid propagation of somatic embryos of Miscanthus hybrid No. 9, which is applicable to Miscella hybrid No. 9 No. young panicle is the seedling obtained from the explant through callus induction, as well as multiplication, rooting, domestication and transplantation. Background technique [0002] Internationally, fossil energy is increasingly scarce, and the pollution caused by fossil energy is also becoming more and more serious. At present, the development and utilization of new energy has become a new issue faced by countries all over the world. In the process of global efforts to find alternative fossil energy and first-generation energy crops, Miscanthus has gradually entered the field of vision of researchers and is expected to become a second-generation energ...

AI Technical Summary

Problems solved by technology

However, Qigang, which is used as an energy plant in Europe and the United States, is a natural hybrid of diploid awn and tetraploid grass. In the 1990s, relevant tissue culture and rapid propagation techniques were reported. Researchers used young leaf , hypocotyl, young root, and young panicle were used as explants to study the rapid propagation technology of Miscanthus plants, but the healing rate and differentiation rate were different. The young panicle had the highest reproductive efficiency, but did not obtain embryogenicity. heal

In addition, in 2005, there was also a report on the tissue culture and rapid propagation technology of Miscanthus using the organogenesis pathway, but no embryogenic callus was obtained.

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