35results about How to "High value-added rate" patented technology

The invention discloses a bletilla direct seeding seedling culture method. The seedling culture method comprises the steps of (1) compost preparation, (2) seeding and water spraying, (3) coverage, (4) irrigation, (5) culture out of a greenhouse, (6) leaf fertilizer spraying, (7) transplanting and (8) harvesting. According to the bletilla direct seeding seedling culture method, the defect that the cost is high under the aseptic environment is overcome, natural cultivation and growth are achieved under the bacteria environment, large-scale and efficient seedling culture can be achieved, bletilla wild resources are protected, damage to natural resources is reduced, the transplanted bletilla can rapidly adapt to the natural environment, the cost is low, the growth rate is high, the method is convenient and easy to implement, the seedling culture cost is greatly reduced, batch production can be achieved, and the application value is high.

The invention discloses application of alpha-mannatide as vaccine adjuvant. The alpha-mannatide which is taken as the vaccine adjuvant has the advantages of being safe, effective, stable and low in price. Firstly, the alpha-mannatide is taken as the immune reinforcing agent to be applied in the clinic, and the main dosage form of the alpha-mannatide is tablet, muscle injection and oral liquid. Therefore, the alpha-mannatide is proved to be safe abundantly, and the alpha-mannatide is safe and reliable within the range of the dosage for immune; secondly, the alpha-mannatide is from a microbe fermentation product, low in price, and good in economic benefit; importantly, the alpha-mannatide can be used for effectively reducing the dosage of the antigen required by the protective immunity; and the higher immunity reaction can be stimulated under the same dosage of the antigen, the cell immune response can be preferably induced particularly, and the alpha-mannatide is good in immune protective function compared with the aluminum adjuvant.

The invention discloses a rapid propagation method for suspension cell culture of Millettia speciosa Champ. According to the rapid propagation method, young and tender cotyledons taken from Millettia speciosa Champ pods 50-60 days after flowering are utilized as culture materials, subcultured callus tissue is obtained through inducing culture and subculture, and a stable Millettia speciosa Champ suspension cell culture fluid is obtained through the suspension cell culture. With the adoption of the method, stable Millettia speciosa Champ suspension cells with good quality and high propagation yield can be obtained, and a novel way and method are provided for development and utilization of effective components of the Millettia speciosa Champ and creation of new germplasm resources from the Millettia speciosa Champ with biotechnology methods such as somatic hybridization, genetic transformation, mutant induction and the like.

The invention relates to a shaft lever part for engineering machinery, comprising a core, a quench hardening layer and an intermediate layer between the quench hardening layer and the core. The surface hardness of the shaft lever part is not less than 50 HRC; the ratio DS / r of the depth DS of the quench hardening layer to the radius r of a shaft lever is 0.28-0.32; and the proportional relation of the depth DS of the quench hardening layer and the width b of the intermediate layer can be expressed as follows: b=(0.382-0.618)DS. The manufacturing method of the shaft lever part for engineering machinery comprises the procedures of tempering and heat treating, rough turning, surface induction quenching and stress-eliminated tempering. The shaft lever part for engineering machinery has stronggrain wearing resistance. The ratio of the depth DS of the hardening layer to the radius r of the shaft lever is 0.28-0.32. The shaft lever part for engineering machinery has highest value-added ratio of torsional strength, highest cost performance, smallest residual stress and longer service life.

The invention belongs to the field of sludge treatment, and particularly relates to a device and a method for treating sludge through earthworm cultivation. An earthworm cultivation device comprises abracket, a plurality of transporting and cultivating units, a feeding end and a discharging end; the plurality of transporting and cultivating units are arranged on the bracket at up-and-down intervals; the feeding end and the discharging end are arranged at the left side end and the right side end of the transporting and cultivating units correspondingly; each transporting and cultivating unit comprises a supporting net, chains, a gear and a motor; the supporting net is used for placing sludge and annularly closed in the left-and-right direction; the chains are arranged at the front end andthe back end of the supporting net and used for driving the supporting net to move; the gear is used for driving the chains to move; the motor is used for driving the gear; a supporting frame is arranged at the feeding end; a foldable transporting plate corresponding to each transporting and cultivating unit is arranged on the supporting frame. Compared with the existing equipment, the earthworm cultivation device adopts automation, is simple in structure, easy to operate and low in manpower demand, and greatly reduces labor cost. Moreover, the occupied area is reduced, the earthworm cultivation efficiency can be effectively improved, the cultivation time is shortened, the yield of the earthworms is increased, energy consumption is reduced and the cultivation cost is reduced.

The invention relates to a rapid propagation method for Chinese roses. The method comprises steps as follows: preparation of an explant, disinfection of the explant, induction culture, multiplication culture, rooting culture and transplanting. The tissue culture method for the Chinese roses is optimized, the production procedures are simplified, the growth cycle is shortened, and the survival rate is increased.

The invention relates to a method and preparation for preparing mature erythrocytes in vitro through peripheral blood, in particular to a method for preparing the mature erythrocytes through Lin-CD34-cells in the peripheral blood. The method includes the steps of enriching the Lin-CD34-cells, differentiating the Lin-CD34-cells into erythroid cells and promoting denucleation and maturation of erythrocytes. In addition, the invention also relates to the mature erythrocytes prepared by the method, a differentiated culture medium and a cytokine combination, wherein the differentiated culture medium and the cytokine combination are applied in the mature erythrocytes. The method for preparation has the advantages that raw materials are easily obtained, the yield of the erythrocytes is high, andthe performance of the obtained erythrocytes is great.

The present invention is the seed separating way, culture method and application of Amphi dinium klebsii as one kind of toxic dinoflagellate. Research shows that there are 60-78 kinds of toxic marine algae and the toxic marine algae have wide application foreground in the research of cancer mechanism and the development of various medicines for resisting cancers, relieving pain, anaesthesia, resisting virus, resisting bacteria, etc. The toxic dinoflagellate under natural environment has very low cell density and this limits its application. For sake of obtaining sufficient material of toxic dinoflagellate, the present invention provides the method of separating pure Amphi dinium klebsii seed from natural water and proliferating in great amount for large scale production.

The invention discloses a method for inducing cluster bud regeneration by means of blueberry stems with buds. The method comprises the specific steps that 1, a one-year-old robust coppice shoot free of pest and disease damage is selected as an explant, disinfected and then segmented, an inducing medium is inoculated with the segments, and after the segments are cultivated for 30 days, cluster buds are obtained and cut off for grafting, cultivation and proliferation; 2, seedlings which are cultured to 5 cm or above are segmented into single plants, a seedling strengthening and rooting medium is inoculated with the single plants, cultivation is conducted for 5 weeks or above, then seedling exercising is conducted, the seedlings are taken out, roots are cleaned, transplanting is conducted, and when the seedlings grow to 20-30 cm high, field planting is conducted; processing of cluster bud regeneration inducing by means of the blueberry stems with the buds is completed through the above-mentioned steps. According to the method, cultivation is easy, the propagation coefficient is high, the regeneration cycle is short, the regenerated plants can be continuously obtained, the cost is effectively reduced, and industrialized seedling cultivating can be achieved.

The invention discloses a method for in vitro intermediate propagation of skimmia reeuesiana. The method comprises the following steps: (1) selecting and disinfecting an explant: by using a mature skimmia reeuesiana seed the explant, disinfecting by using 70% alcohol and then disinfecting by using 0.1% HgCl2 and washing by using sterile water; (2) seed germination: inoculating the disinfected seed into germination culture for culturing, wherein the seed germinates to enable cotyledon and euphylla to emerge; (3) calluses induction and proliferation: slicing a terminal bud with the euphylla, inoculating to a proliferation medium for subculture, wherein calluses are continuously proliferated; (4) rooting culture: inoculating a test-tube plantlet obtained by expanding propagation to a rooting medium and culturing the seedling to differentiate a white adventitious root; and (5) domestication and transplanting. The method is simple, convenient and feasible, great in propagation coefficient, low in production cost and short in period. The average propagation coefficient reaches 4.5, the rooting percentage is 90% and the survival rate of transplanting is 95%. The method is simple and convenient to operate and great in propagation coefficient and lays a foundation for industrial production of skimmia reeuesiana sprouts.

The invention discloses a culture method of cymbidium, aiming at solving the problems that when the cymbidium is bred by a tissue culture technique at present, mutation is inevitably produced, so that the seedling quality is influenced, and the breeding speed of protocorm-like bodies is limited. The culture method comprises the following steps: inducing the protocorm-like bodies by bud apical meristem, carrying out subculture multiplication of the protocorm-like bodies, carrying out differentiation on the protocorm-like bodies to produce adventitious buds, and carrying out strong seedling culture on the adventitious buds to obtain seedlings. After the culture method is used, the problem of mutation in the seedling production of the cymbidium can be effectively solved, the occurrence of mutation is reduced, the value-added ratio is increased, more than half of the subculture multiplication time is shortened, and the multiplication rate of the protocorm-like bodies is improved. The test proves that the value-added rate of the protocorm-like bodies is increased by 80-120%, the mutational rate is reduced by 5-9%, and the problem of contradiction between the value-added rate and the mutational rate of the protocorm-like bodies of the cymbidium can be effectively solved. The culture method has important significance for the rapid breeding and industrial large-scale production of the seedlings of the cymbidium, and has better market prospects and application values.

The invention discloses a method and device for improving the tritium value-added rate of a fusion reactor. The method comprises the following steps: acquiring a fusion reactor divertor geometric configuration, a vacuum chamber and cladding interface, a divertor teleoperation maintenance strategy, a divertor material route map and divertor load input; determining a divertor basic structure model; wherein the divertor basic structure model weakens the neutron shielding capability of the divertor on the premise that components meet the structural strength, and a target plate component structure model is determined; determining a hybrid divertor cladding final structure model, wherein a shielding block mounted between the divertor and the vacuum chamber is canceled; and determining the tritium value-added rate increasing quantity. The device comprises a bottom cladding, an outer target plate component, a Dome component and an inner target plate component, and the outer target plate component, the Dome component and the inner target plate component are directly connected with the bottom cladding. According to the invention, the tritium production area of the cladding is expanded, and the tritium increment rate of at least 0.04 can be expected to be improved through numerical simulation evaluation, so that the influence of a heating and diagnosis system on cladding opening can be made up.

The invention discloses a method for rapidly breeding miscanthus plant miscanthus giganteus embryo by tissue culture, comprising the following steps: A, selecting a young ear; B, carrying out primary induction, namely cutting the young ear into sections, sterilizing with alcohol, soaking with mercury chloride, then washing with sterile water, and inoculating the sections onto a primary induction culture medium of the young ear; C, carrying out subculture for seedling growth, namely carrying out successive transfer seedling growing on the obtained embryonic callus onto a subculture medium, carrying out subculture once, and transferring the embryonic callus onto a seedling growing culture medium, thus small young seedling is formed; D, carrying out root generating induction, namely inducting the young seedling on a root growing induction culture medium to generate a root, culturing, and transferring the young seedling onto a root growing culture medium, thus the young seedling differentiates white or yellow roots; and E, carrying out acclimatization and transplanting, namely transplanting the seedling into a flower pot filled with vermiculite and soil to be acclimatized, spraying acclimatization culture liquid and clear water onto the young seedling regularly, and transferring the seedling into a seedling nursery. The method is feasible and is simple to operate, the rapid breeding efficiency and the breeding coefficient of miscanthus giganteus are improved in short time, the appreciation is stable, the root system is robust, and the transplanting survival rate is high.

The invention relates to a stevia rebaudiana tissue culture propagation method and a culture medium of the method, and particularly relates to a tissue culture rapid propagation method for directly forming a bud by using a stevia rebaudiana leaf as an explant. The stevia rebaudiana tissue culture method comprises the following steps: (1), collecting the explant; (2), disinfecting and inoculating; (3), performing thickening induction-culturing; (4), budding and seedling; (5), transferring and grafting, and rooting; and (6), hardening and transplanting. According to the stevia rebaudiana tissue culture method provided by the invention, materials are easily available, a plurality of seed sources can be rapidly propagated, the explant tissue culture has no culture phase of callus cell proliferation and differentiation, thus the culture time is shortened, extremely high reproduction rate is achieved; the stevia rebaudiana tissue culture propagation method is easy to operate and is capable of realizing large-scale production; and the operation of researches such as genetic transformation is facilitated in a culture process. The culture medium provided by the invention ensures that the leaf is capable of reaching the expected culture target in each phase.

The invention discloses a proliferation method of broccoli, comprising the following steps: 1) removing the root of broccoli, marking the broccoli plant of which the root is removed as a root-removed broccoli plant; 2) implanting the root-removed broccoli plant into an enrichment medium, cultivating until the root-removed broccoli plant outgrows a root and a terminal bud, and marking the obtained plant as a broccoli plant with the root and the terminal bud; 3) removing the terminal bud of the broccoli plant with the root and the terminal bud, continuing to cultivate until the root of the leaf blade of the plant outgrows a bud point, and marking the plant in the period as a broccoli plant with the bud point; and 4) cutting off the bud point of the broccoli plant with the bud point, and inoculating in a rooting medium to be cultivated until the broccoli seedling is obtained. Experiments prove that the method of the invention has short proliferating cycle, high proliferating rate and high survival rate of the obtained broccoli seedling. The method of the invention has wide application prospect in the broccoli proliferation field.

The invention discloses a bletilla direct seeding seedling culture method. The seedling culture method comprises the steps of (1) compost preparation, (2) seeding and water spraying, (3) coverage, (4) irrigation, (5) culture out of a greenhouse, (6) leaf fertilizer spraying, (7) transplanting and (8) harvesting. According to the bletilla direct seeding seedling culture method, the defect that the cost is high under the aseptic environment is overcome, natural cultivation and growth are achieved under the bacteria environment, large-scale and efficient seedling culture can be achieved, bletilla wild resources are protected, damage to natural resources is reduced, the transplanted bletilla can rapidly adapt to the natural environment, the cost is low, the growth rate is high, the method is convenient and easy to implement, the seedling culture cost is greatly reduced, batch production can be achieved, and the application value is high.

The invention relates to the technical field of plant culture, particularly to a strawberry subculture multiplication medium and application thereof. The subculture multiplication medium per liter comprises the following raw materials: 0.5mg of 6-benayl aminopurine, 0.01mg of naphthylacetic acid, 30g of white sugar, 3.8g of agar and the balance of an MS culture medium. Then, the subculture multiplication medium is used for multi-generation subculture multiplication in a strawberry virus-free tissue culture and rapid propagation method. With adoption of the subculture multiplication medium provided by the invention, the value-added ratio of strawberry plantlets is increased by about eight times, the average plant height is increased by about 25.85%, the average number of blades is increased by about 16.03%, the average number of stolons multiplied by a single plant is increased by about 109.51%, the final strawberry yield is increased by about 53.98%, and the number of diseased strawberries and the survival rate of the strawberries are both remarkably increased; in addition, the subculture multiplication medium provided by the invention is convenient in application, low in cost and easy to promote and popularize, and the production enthusiasm of strawberry planters is remarkably improved.

The invention provides a compound as well as a preparation method and application thereof. The compound has a structure shown as formula (I), and the compound can treat osteoporosis; moreover, by virtue of cell experiments, the compound is discovered to have an effect on promoting the proliferation of osteoblasts; and when the sample concentration is 10mu M, the proliferation rate can reach 11.24 percent.

The invention belongs to the technical field of biochemistry, and provides a preparation method of human renal podocytes. The human renal podocytes conforms to an expression result of a human renal podocyte marker; and meanwhile, the preparation method is good in repeatability and simple to operate, and the human renal podocytes with high yield, higher value-added rate and high purity can be obtained.

The invention discloses a method for cultivating Cymbidium cymbidium, aiming to solve the problem that when the current tissue culture technique is used to propagate Cymbidium cymbidium, variation inevitably occurs, which affects the quality of seedlings and limits the propagation speed of protocorms. The method comprises the following steps: using bud top clone tissue to induce protocorm-like, protocorm-like subculture multiplication, protocorm-like differentiation to produce adventitious buds, and cultivating strong adventitious buds to obtain seedlings. The invention can effectively solve the variation problem in the production of Cymbidium cymbidium seedlings, reduce the occurrence of variation, increase the value-added rate, shorten the subculture time by more than half, and increase the protocorm-like multiplication rate in a disguised form. It is determined that the invention increases the value-added rate of protocorm-like protocorms by 80-120%, and reduces the variation rate by 5-9%, effectively solving the contradiction between the value-added rate and the variation rate of the protocorm-like Cymbidium. The invention has important significance for rapid propagation of Cymbidium seedlings and industrialized large-scale production, and has good market prospect and application value.

The invention provides a cell culture bottle, which comprises a bottle body, a partition is horizontally arranged in the bottle body, the partition divides the interior of the bottle body into an upper chamber and a lower chamber, an open upper chamber tube is arranged on the upper part of the side of the upper chamber, an open lower chamber tube is arranged on the upper part of the side of the lower chamber, the open upper chamber tube and the open lower chamber tube are provided with seal covers or air-permeable covers, and a dialysis membrane is arranged on the partition. The cell culture bottle provided by the invention decreases the accumulation of metabolic waste concentration, increases the proliferation rate and viability of cells, and ensures a good cell culture environment, and moreover, the cell culture bottle can prevent operational pollution caused by repeated medium replenishment, and saves the time and energy of laboratory technicians.

The invention provides a method for detoxification micropropagation of callicarpa nudiflora, and relates to solve the problems of long propagation period of the callicarpa nudiflora, low propagation efficiency and high cost by using a method of plant detoxification micropropagation and tissue culture. The method comprises the steps of (1) detoxification of explants; (2) culture of aseptic seedlings; (3) culture of micro branches: transplanting proliferated aseptic seedlings into a seedling-strengthening culture medium to culture; (4) propagation culture: cutting the strengthened micro branches into segments with one apical bud or 1-2 axillary buds for culture; (5) rooting culture: transplanting the micro branches to a rooting culture medium when the growth length of the micro branches reaches 2.0-3.0 cm; (6) hardening culture: hardening the rooting cultured callicarpa nudiflora seedlings in a sunshelter for 7-10 d; and (7) transplanting callicarpa nudiflora seedlings after hardening of the seedlings into seedling cups to finish detoxification micropropagation of the callicarpa nudiflora. The method for the detoxification micropropagation of the callicarpa nudiflora has the advantages of short propagation cycle, high propagation rate, identical seedling characteristics, low cost, and high rooting rate and survival rate of transplanted seedlings. Besides, the growth cycle is not restricted by seasons.

The invention relates to a shaft lever part for engineering machinery, comprising a core, a quench hardening layer and an intermediate layer between the quench hardening layer and the core. The surface hardness of the shaft lever part is not less than 50 HRC; the ratio DS / r of the depth DS of the quench hardening layer to the radius r of a shaft lever is 0.28-0.32; and the proportional relation of the depth DS of the quench hardening layer and the width b of the intermediate layer can be expressed as follows: b=(0.382-0.618)DS. The manufacturing method of the shaft lever part for engineering machinery comprises the procedures of tempering and heat treating, rough turning, surface induction quenching and stress-eliminated tempering. The shaft lever part for engineering machinery has strong grain wearing resistance. The ratio of the depth DS of the hardening layer to the radius r of the shaft lever is 0.28-0.32. The shaft lever part for engineering machinery has highest value-added ratio of torsional strength, highest cost performance, smallest residual stress and longer service life.

The invention relates to a composition, a culture medium supplement, a stem cell culture medium and a stem cell culture method. The composition is prepared from L-ascorbic acid-2-trisodium phosphate, catalase, coenzyme Q10, rapamycin and apocynin. According to the invention, L-ascorbic acid-2-trisodium phosphate, catalase, coenzyme Q10, rapamycin and apocynin are selected to be combined to form a composition with a specific formula, and the composition and non-essential amino acids are added into a serum-free culture medium to form a specific serum-free culture system. When the serum-free culture system is adopted to perform subculture on ADSCs, the cell proliferation rate can be higher than that of a serum-containing culture system. Meanwhile, in subculture, cell vacuolation is effectively inhibited, cell senescence is relieved, and the effect can reach the level equivalent to that of a serum culture medium.

The invention discloses a method for quickly reproducing miscanthus plant miscanthus and triarrhena hybrid NO.9 somatic embryo tissue culture, which comprises the following steps of: (1) selecting young ears: selecting young ears of miscanthus and triarrhena hybrid NO.9 staying in the gramineae primordium formation period; (2) inducing the young ears by using a primary induction culture medium: cutting the young ears into small segments, disinfecting the segments with alcohol, soaking the segments in mercuric chloride, flushing the segments with sterile water, inoculating the segments on the primary induction culture medium of young ears, and inducing primary generation to obtain embryonal calluses; (3) cubculturing by using a seedling culture medium: subculturing the obtained embryonal calluses on a subculture medium; (4) inducing to grow roots by using a root growing induction culture medium: inducing the grown seedlings on the induction culture medium to grow roots; and (5) domesticating and transplanting: spraying a domesticating culture liquid and clean water to the seedlings regularly every day in a greenhouse, transplanting the seedlings to a nursery, and illuminating the seedlings to obtain the seedlings of miscanthus and triarrhena hybrid NO.9. The method is feasible and simple and convenient to operate, the differentiation callus induction rate and the qualified seedling percentage are high, the survival rate is high, and the method is applicable to quick reproduction in the industrial production of miscanthus and triarrhena hybrid NO.9 seedlings.