Method for tissue culturing and quick propagation of sugarcane with intermittent immersion bioreactor

Abstract

The invention provides to a method for tissue culturing and quick propagation of sugarcane with an intermittent immersion bioreactor, which is characterized in that: the method performs propagation culture and rooting culture by transplanting detoxicated sugarcane plants, serving as materials, cultured by generations from induced sprouts of sugarcane stem tip tissues into the intermittent immersion bioreactor. In the intermittent immersion bioreactor, a culture temperature is 29 DEG C; the illumination intensity is 1,500 Lx and the illumination time is 16 hours; an intermittent culture condition is to culture for 3min every 3h and to root for 3min every 6h; the first generation can be propagated to 40 times; after rooting is completed, a proper hardening plantlet treatment is performed; and plantlets are transplanted and the survival rate is about 85 percent. The set of system is high in automation degree, has a much higher multiplication rate than that of a traditional tissue culturemethod, saves labour cost and increases economic benefits.

Description

technical field [0001] The invention relates to a method for obtaining healthy sugarcane seedlings through tissue culture rapid propagation of sugarcane (Xintaisu 22, Xintaisu 16, Badila) by using an intermittent submerged bioreactor. Background technique [0002] Sugarcane (Saccharum officenarum L.) belongs to the monocotyledonous plant class (Liliopsida), Epiflora (Gluimflorae), Poaceae (Gramineae) Saccharum (Saccharum). Sugarcane is one of 10 genera in the sugarcane subfamily of the Shu subgroup of Poaceae. It forms a Saccharum complex closely related to sugarcane breeding with Saccharomyces, Miscanthus, Asteria and Hebawangwu. It mainly includes tropical species, Indian species, Chinese species, thin-stem wild species, and large-stem wild species. The first three are cultivated species, and the latter two are wild species. [0003] The plants of sugarcane are composed of roots, stems, leaves, flowers and fruits (seeds). Flowers and seeds are meaningful in the improveme...

AI Technical Summary

Problems solved by technology

[0009] 1. The value-added rate is low. Due to the relatively low reproduction coefficient of traditional tissue culture technology, it is generally 2.0-4.0 per generation, and the propagation of a large number of tissue culture seedlings requires a higher number of generations, generally about 10-12 generations. As the subgeneration number increases, the mutation rate of the tissue culture seedlings also increases, while the quality and resistance of the tissue culture seedlings decrease. However, sufficient economic benefits cannot be obtained without increasing the subgeneration number in production. Therefore, in order to pursue economic interests, industrialization In the production of sugarcane tissue culture seedlings, some inferior seedlings caused by too many generations are put on the market

[0010] 2. The level of automation is low and labor-intensive. Because the traditional sugarcane tissue culture production process requires a higher number of generations of multiplication, it usually needs to be transferred once every 20 days, requiring a lot of manual transfer and subculture, thereby increasing labor costs and causing production The cost increases, and the traditional method requires a large number of tissue culture bottles, which not only takes up a lot of space to display, but also requires a lot of manual operation in the process of operation

[0011] 3. The production cycle is long. Due to the relatively low reproduction coefficient of the traditional tissue culture method, it generally takes about 10 months to cultivate after 10 to 12 generations.

[0012] 4. The production cost is high. Most of the production cost of traditional sugarcane tissue culture is labor cost, accounting for about 65% of the whole production cost, and most of the labor cost is the cost of transfer work. Therefore, the labor cost in the transfer work accounts for a large proportion, resulting in an increase in production costs

[0013] The above points are some of the most important problems encountered in sugarcane production at present. They have been highlighted in the production process, and they have gradually hindered the healthy development of sugarcane tissue culture production. First, due to proliferation The coefficient is not high, leading to an increase in the number of multiplication generations, which easily leads to the quality of tissue culture seedlings not being guaranteed, and it is easy to put some inferior seedlings on the market; secondly, the level of automation is low, labor-intensive, and requires a lot of labor costs to spend on tissue culture In the transfer work of seedlings, this leads to an increase in production costs; finally, due to the increase in the number of generations, the production cycle is extended, and the supply of tissue culture seedlings cannot be reasonably allocated, and production cannot be flexibly responded to market demand.

Due to the low multiplication coefficient, heavy labor, low level of automation, and relatively high production costs, traditional methods are difficult to adapt to the large-scale production of sugarcane tissue culture seedlings in the future, and the use of interstitial submerged bioreactors for sugarcane tissue culture seedlings The production can overcome the defects of traditional methods, and provide guarantee for the low-cost production of high-quality sugarcane tissue culture seedlings