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Culture method of cymbidium

A culturing method and a technology for Cymbidium, applied in the field of orchid cultivation and agriculture, can solve the problems of variation, affecting the quality of seedlings, limiting the reproduction speed of protocormoid, etc., so as to improve the proliferation rate, shorten the subculture time, and ensure the seedlings. quality effect

Active Publication Date: 2015-08-05
MIANYANG XIANLONG BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of cultivation method for Cymbidium phalanx in view of the problems that the current propagation of Cymbidium cymbidium by tissue culture technology inevitably produces variation, affects the quality of seedlings, and limits the propagation speed of protocorms. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Huofenghuang is a very popular variety of Cymbidium, but during the tissue culture process, the protocorm-like proliferation rate is slow and the mutation rate is high. This variety is used as an example to illustrate the effect of this technology in actual production.

[0029] Configure liquid proliferation medium, wherein the concentration of each component is: 500mg / L (NH 4 ) 2 SO 4 , 250mg / L MgSO 4 ·7H 2 O, 525mg / L KNO 3 , 250mg / L KH 2 PO 4 、200mg / L Ca 3 (PO 4 ) 2 , 28mg / L Fe 2 (C 4 h 4 o 6 ) 3 2H 2 O, 7.5mg / L MnSO 4 4H 2 0. 20000mg / L sugar, adjust the pH value of the liquid proliferation medium to 5.8, and use a high-pressure steam sterilizer to sterilize for about 25 minutes.

[0030] Configure solid proliferation medium, wherein the concentration of each component is: 500mg / L (NH 4 ) 2 SO 4 , 250mg / L MgSO 4 ·7H 2 O, 250mg / L KH 2 PO 4 , 200mg / L Ca(NO 3 ) 2 4H 2 O, 28mg / L FeSO4 ·7H 2 O, 7.5mg / L MnSO 4 4H 2 O, 0.2mg / L 6-BA, 0.1mg / L NAA,...

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Abstract

The invention discloses a culture method of cymbidium, aiming at solving the problems that when the cymbidium is bred by a tissue culture technique at present, mutation is inevitably produced, so that the seedling quality is influenced, and the breeding speed of protocorm-like bodies is limited. The culture method comprises the following steps: inducing the protocorm-like bodies by bud apical meristem, carrying out subculture multiplication of the protocorm-like bodies, carrying out differentiation on the protocorm-like bodies to produce adventitious buds, and carrying out strong seedling culture on the adventitious buds to obtain seedlings. After the culture method is used, the problem of mutation in the seedling production of the cymbidium can be effectively solved, the occurrence of mutation is reduced, the value-added ratio is increased, more than half of the subculture multiplication time is shortened, and the multiplication rate of the protocorm-like bodies is improved. The test proves that the value-added rate of the protocorm-like bodies is increased by 80-120%, the mutational rate is reduced by 5-9%, and the problem of contradiction between the value-added rate and the mutational rate of the protocorm-like bodies of the cymbidium can be effectively solved. The culture method has important significance for the rapid breeding and industrial large-scale production of the seedlings of the cymbidium, and has better market prospects and application values.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to the field of orchid cultivation, in particular to a method for cultivating Cymbidium cymbidium. The invention can effectively solve the variation problem in the cultivation of the Cymbidium cymbidium, and has good effect. Background technique [0002] Cymbidium grandifloruim (Cymbidium grandifloruim) is a large-flowered epiphytic species in the genus Cymbidium. It has rich and gorgeous colors, tall and straight flower branches, huge flowers, super long flowering period, light fragrance, and noble temperament. , has extremely high ornamental value and commodity value, and is deeply loved by people all over the world. [0003] Cymbidium has long been widely planted in Japan, South Korea, the United States, New Zealand, Europe, Taiwan and other places, and the Cymbidium industry in my country is also booming, with annual sales of more than one million pots. With the vigorous development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李茜茜曾玉冰
Owner MIANYANG XIANLONG BIOTECH CO LTD
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