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Proliferation method of broccoli

A technology for broccoli and proliferation medium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as plant poisoning, difficult evaluation, adverse effects of subsequent experiments, etc., achieve high survival rate, avoid inhibition, Contrasting results with credible effects

Inactive Publication Date: 2010-09-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the germination medium propagation method, the germination medium contains Ag + , Ag + It will have certain toxic effects on plants, such as inhibiting plant growth and forming deformed plants, and this effect is difficult to evaluate, and may have adverse effects on subsequent experiments

Method used

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  • Proliferation method of broccoli
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  • Proliferation method of broccoli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, broccoli multiplication

[0035] Proliferation medium is prepared as follows: add 40g sucrose, 10g agar and 0.1mg IAA to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain proliferation medium; 121.3 degrees Celsius, 15-20 minutes Autoclave.

[0036] The rooting medium was prepared as follows: add 30 g of sucrose and 8 g of agar to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain the rooting medium; sterilize under high pressure at 121.3 degrees Celsius for 15 to 20 minutes.

[0037] 1. Proliferation

[0038] The following broccoli plants with hypocotyls 7 cm long were used as starting material for propagation. The propagation steps are as follows:

[0039] 1) In the ultra-clean bench, the broccoli plant with hypocotyl 7cm long is derooted, and the broccoli plant after derooting is denoted as the derooted broccoli plant;

[0040] 2) The broccoli plant without root is inserted into the proliferation medium, cultivated, unti...

Embodiment 2

[0065] Embodiment 2, broccoli multiplication

[0066] Proliferation medium is prepared as follows: add 40g sucrose, 10g agar and 0.1mg IAA to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain proliferation medium; 121.3 degrees Celsius, 15-20 minutes Autoclave.

[0067] The rooting medium was prepared as follows: add 30 g of sucrose and 8 g of agar to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain the rooting medium; sterilize under high pressure at 121.3 degrees Celsius for 15 to 20 minutes.

[0068] 1. Proliferation

[0069] Broccoli plants with a hypocotyl length of 3 cm were used as starting material for propagation. The propagation steps are as follows:

[0070] 1) In the ultra-clean bench, remove the root of the broccoli plant with 3 cm long hypocotyl, and denote the broccoli plant after the root removal as the rootless broccoli plant;

[0071] 2) The broccoli plant without root is inserted into the proliferation medium, cultivated, a...

Embodiment 3

[0096] Embodiment 3, broccoli multiplication

[0097] Proliferation medium is prepared as follows: add 40g sucrose, 10g agar and 0.1mg IAA to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain proliferation medium; 121.3 degrees Celsius, 15-20 minutes Autoclave.

[0098] The rooting medium was prepared as follows: add 30 g of sucrose and 8 g of agar to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain the rooting medium; sterilize under high pressure at 121.3 degrees Celsius for 15 to 20 minutes.

[0099] 1. Proliferation

[0100] Broccoli plants with 5 cm long hypocotyls were used as starting material for propagation. The propagation steps are as follows:

[0101] 1) In the ultra-clean bench, remove the root of the broccoli plant with 5 cm long hypocotyl, and denote the broccoli plant after the root removal as the rootless broccoli plant;

[0102] 2) the broccoli plant without root is inserted into the proliferation medium, and cultivated unti...

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Abstract

The invention discloses a proliferation method of broccoli, comprising the following steps: 1) removing the root of broccoli, marking the broccoli plant of which the root is removed as a root-removed broccoli plant; 2) implanting the root-removed broccoli plant into an enrichment medium, cultivating until the root-removed broccoli plant outgrows a root and a terminal bud, and marking the obtained plant as a broccoli plant with the root and the terminal bud; 3) removing the terminal bud of the broccoli plant with the root and the terminal bud, continuing to cultivate until the root of the leaf blade of the plant outgrows a bud point, and marking the plant in the period as a broccoli plant with the bud point; and 4) cutting off the bud point of the broccoli plant with the bud point, and inoculating in a rooting medium to be cultivated until the broccoli seedling is obtained. Experiments prove that the method of the invention has short proliferating cycle, high proliferating rate and high survival rate of the obtained broccoli seedling. The method of the invention has wide application prospect in the broccoli proliferation field.

Description

technical field [0001] The invention relates to a method for multiplying broccoli. Background technique [0002] In the prior art, the propagation method of broccoli (Brassica oleracea var. italica) generally is following two kinds: 1, use germination medium to carry out propagation, and this method is to produce new bud point from the base of broccoli plant, utilize bud Point multiplication; 2. Natural multiplication. The natural multiplication speed is very slow, generally not as a special multiplication method. In the germination medium propagation method, the germination medium contains Ag + , Ag + It will have certain toxic effects on plants, such as inhibiting plant growth and forming deformed plants, and this effect is difficult to evaluate, and may have adverse effects on subsequent experiments. Therefore, there is a need for a safe and rapid broccoli multiplication method. Contents of the invention [0003] One object of the present invention is to provide a ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郭仰东杨鹏刘莉莎王玉珏温常龙赵立群程琳
Owner CHINA AGRI UNIV
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