Proliferation method of broccoli
A technology for broccoli and proliferation medium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as plant poisoning, difficult evaluation, adverse effects of subsequent experiments, etc., achieve high survival rate, avoid inhibition, Contrasting results with credible effects
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Embodiment 1
[0034] Embodiment 1, broccoli multiplication
[0035] Proliferation medium is prepared as follows: add 40g sucrose, 10g agar and 0.1mg IAA to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain proliferation medium; 121.3 degrees Celsius, 15-20 minutes Autoclave.
[0036] The rooting medium was prepared as follows: add 30 g of sucrose and 8 g of agar to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain the rooting medium; sterilize under high pressure at 121.3 degrees Celsius for 15 to 20 minutes.
[0037] 1. Proliferation
[0038] The following broccoli plants with hypocotyls 7 cm long were used as starting material for propagation. The propagation steps are as follows:
[0039] 1) In the ultra-clean bench, the broccoli plant with hypocotyl 7cm long is derooted, and the broccoli plant after derooting is denoted as the derooted broccoli plant;
[0040] 2) The broccoli plant without root is inserted into the proliferation medium, cultivated, unti...
Embodiment 2
[0065] Embodiment 2, broccoli multiplication
[0066] Proliferation medium is prepared as follows: add 40g sucrose, 10g agar and 0.1mg IAA to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain proliferation medium; 121.3 degrees Celsius, 15-20 minutes Autoclave.
[0067] The rooting medium was prepared as follows: add 30 g of sucrose and 8 g of agar to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain the rooting medium; sterilize under high pressure at 121.3 degrees Celsius for 15 to 20 minutes.
[0068] 1. Proliferation
[0069] Broccoli plants with a hypocotyl length of 3 cm were used as starting material for propagation. The propagation steps are as follows:
[0070] 1) In the ultra-clean bench, remove the root of the broccoli plant with 3 cm long hypocotyl, and denote the broccoli plant after the root removal as the rootless broccoli plant;
[0071] 2) The broccoli plant without root is inserted into the proliferation medium, cultivated, a...
Embodiment 3
[0096] Embodiment 3, broccoli multiplication
[0097] Proliferation medium is prepared as follows: add 40g sucrose, 10g agar and 0.1mg IAA to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain proliferation medium; 121.3 degrees Celsius, 15-20 minutes Autoclave.
[0098] The rooting medium was prepared as follows: add 30 g of sucrose and 8 g of agar to 1L MS medium, mix well, and adjust its pH value to 6.2 to obtain the rooting medium; sterilize under high pressure at 121.3 degrees Celsius for 15 to 20 minutes.
[0099] 1. Proliferation
[0100] Broccoli plants with 5 cm long hypocotyls were used as starting material for propagation. The propagation steps are as follows:
[0101] 1) In the ultra-clean bench, remove the root of the broccoli plant with 5 cm long hypocotyl, and denote the broccoli plant after the root removal as the rootless broccoli plant;
[0102] 2) the broccoli plant without root is inserted into the proliferation medium, and cultivated unti...
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