A kind of cultivation method of Cymbidium
A cultivation method, the technology of Cymbidium, applied in the field of cultivation of Cymbidium, can solve the problems of limiting protocorm propagation speed, variation, and affecting the quality of seedlings, so as to shorten the subculture time, increase the proliferation rate, reduce the The effect of training costs
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[0028] Huofenghuang is a very popular variety of Cymbidium, but during the tissue culture process, the protocorm-like proliferation rate is slow and the mutation rate is high. This variety is used as an example to illustrate the effect of this technology in actual production.
[0029] Configure liquid proliferation medium, wherein the concentration of each component is: 500mg / L (NH 4 ) 2 SO 4 , 250mg / L MgSO 4 ·7H 2 O, 525mg / L KNO 3 , 250mg / L KH 2 PO 4 、200mg / L Ca 3 (PO 4 ) 2 , 28mg / L Fe 2 (C 4 h 4 o 6 ) 3 2H 2 O, 7.5mg / L MnSO 4 4H 2 0. 20000mg / L sugar, adjust the pH value of the liquid proliferation medium to 5.8, and use a high-pressure steam sterilizer to sterilize for about 25 minutes.
[0030] Configure solid proliferation medium, wherein the concentration of each component is: 500mg / L (NH 4 ) 2 SO 4 , 250mg / L MgSO 4 ·7H 2 O, 250mg / L KH 2 PO 4 , 200mg / L Ca(NO 3 ) 2 4H 2 O, 28mg / L FeSO4 ·7H 2 O, 7.5mg / L MnSO 4 4H 2 O, 0.2mg / L 6-BA, 0.1mg / L NAA,...
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