A kind of efficient propagation method of Gerbera detoxification seedlings
A technology of virus-free seedlings and gerbera, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low survival rate of seedling transplanting, low induction rate, poor quality of induced seedlings, etc., and achieve transplant survival The effect of high efficiency, simple disinfection method and high seedling quality
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Embodiment 1
[0049] The high-efficiency multiplication method of the virus-free gerbera seedlings of the present embodiment was carried out in 2014-2015. The pre-treatment, plant regeneration and seedling hardening of the gerbera gerbera virus-free seedlings were carried out in Lianyungang Zhenxing Flower Garden, and the tissue-cultured seedlings were transplanted Planted in the flower experiment base of Dongxin Farm in Lianyungang City, the detailed steps are as follows:
[0050] 1) Gerbera detoxification pre-emergence treatment:
[0051] The virus-free seedlings of the gerbera variety Sunshine Coast were used to wash them repeatedly with tap water, then washed with a brush dipped in detergent, then rinsed with ultra-pure water, soaked in 70% alcohol for 15 seconds, and then rinsed with sterile water for two times. Add a few drops of Tween 20 solution to 0.1% mercuric chloride and soak for 5 minutes, rinse with sterile water for 5 times, blot dry with filter paper, and transfer the petiol...
Embodiment 2
[0068] The high-efficiency propagation method of Gerbera chrysanthemum virus-free seedlings in this embodiment, implementation time, pre-treatment of gerbera chrysanthemum virus-free seedlings, plant regeneration, seedling hardening, and tissue culture seedling transplanting are all the same as in Example 1, and the culture medium (liquid) The formulation, comparison medium (solution) formulation and operation steps are the same as in Example 1. The biggest difference between this example and Example 1 is that the Gerbera chrysanthemum virus-free seedling variety is replaced by Linglong:
[0069] 1) Gerbera detoxification pre-emergence treatment:
[0070] The virus-free seedlings of the gerbera variety Linglong are used to wash them repeatedly with tap water, wash them once with a brush dipped in detergent, then rinse them with ultra-pure water, soak them in 70% alcohol for 15 seconds, and rinse them twice with sterile water , add a few drops of Tween 20 solution to 0.1% mercu...
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