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Rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant

A plant and awn body technology, which is applied in the field of tissue culture and rapid propagation of Miscanthus penicillium somatic embryos, can solve the problems of different healing rate and differentiation rate, failure to obtain embryogenicity, and failure to obtain embryogenicity, etc., to achieve healing The effect of high wound reproduction rate, high value-added rate and stable value-added

Inactive Publication Date: 2012-11-21
湖北光芒能源植物有限公司
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Problems solved by technology

[0002] In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproductive efficiency of the young panicle is the highest, but no embryogenic callus has been obtained.
In addition, in 2005, there was also a report on the tissue culture and rapid propagation technology of Miscanthus plants. The stem cutting technology was used to obtain seedlings, but this is also the organogenesis pathway used, and no embryogenic callus was obtained.

Method used

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  • Rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant
  • Rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant
  • Rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant

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Embodiment 1

[0025] A method for rapid propagation of somatic embryo tissue culture of Miscanth acanthus, the steps of which are:

[0026] 1. Selection of young panicles: Select young panicles that are in the formation stage of Yinghua primordium. The seeds in the young panicles have not yet formed, the central axis is relatively soft, and the basic branches have not yet formed.

[0027] 2. Use the primary culture medium for primary induction: cut it into 2-3 cm pieces, first sterilize it with 75% alcohol for 30 seconds, then soak it in 0.1% mercury chloride for 5 minutes, then rinse it with sterile water three times, and inoculate it On the primary induction medium of young panicles. After 35 days of primary induction, embryogenic callus was obtained. The culture conditions are 25°C, 16 hours of light per day, and the light intensity is 2000LX. The primary induction medium is: A: MS medium supplemented with kinetin KT1-5mg / L, naphthaleneacetic acid NAA0.1-0.5mg / L, sucrose 30g / L, Phytage...

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Abstract

The invention discloses a rapid tissue culture propagation method for miscanthus floridulu somatic embryo of mscanthus plant, comprising the following steps: A, selecting young ear: selecting the yound ear at Ying flower primordium formation period; B, carrying out primary induction with a primary culture medium: cutting to small sections, sterilizing with alcohol, soaking with mercuric chloride,washing and inoculating on the primary induction culture medium; C, subculturing seedling with a seedling culture medium: subculturing and generating the seedling on a subculture culture medium with the obtained embryonic callus, subculturing, multiplication and shining every day; D, carrying out radication induction with a radication induction culture medium; carrying out the induction radication on the radication induction culture medium by seedling, culturing and shining every day; E, acclimating and transplanting: transplanting the tissue culture seeding to an acclimation nursery garden consisting of vermiculite to acclimate, spraying acclimation culture solution and clean liquid to the seeding at fixed time every day, transplanting to the nursery garden and obtaining the miscanthus floridulu seeding. The method is easy to apply, simple to operate, and large in breed coefficient; and the recovery reproduction rate is high, which is about 100%.

Description

technical field [0001] The present invention relates to five-jointed awn ( Miscanthus floridulu ) technology field of tissue culture and rapid propagation based on embryogenic callus, more specifically relates to a method for rapid propagation of somatic embryos of Miscanthus penicillium through tissue culture, which is suitable for callus induction using young ears of Penicillium as explants Ways to obtain seedlings, as well as value addition, rooting, domestication and transplanting. Background technique [0002] In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproductive efficiency of the young panicle is the highest, but no embryogenic callus was obtained. In addition, in 2005, there was also a report on the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刁英胡中立赵玲玲胡晖胡小虎周发松
Owner 湖北光芒能源植物有限公司
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