A kind of high-efficiency propagation method of virus-free dahlia seedlings
A technology for dahlia and virus-free seedlings, applied in the field of agricultural science, can solve the problems of low seedling survival rate, low induction rate, poor quality of induced seedlings, etc. high effect
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[0046] The high-efficiency propagation method of dahlia virus-free seedlings in this embodiment was implemented in 2012-2013. The pre-treatment, plant regeneration and seedling hardening of dahlia virus-free seedlings were carried out in Lianyungang City Zhenxing Flower Garden, and the tissue-cultured seedlings were transplanted in It is carried out in the flower test base of Dongxin Farm in Lianyungang City. The detailed steps are as follows:
[0047] 1) Dahlia virus-free pre-seedling treatment:
[0048] Rinse the detoxified dahlia seedlings with tap water repeatedly first, wash once with a brush dipped in detergent, then rinse with ultrapure water, soak in 70% alcohol for 15 seconds, then rinse twice with sterile water, 0.1% mercury liter Add a few drops of Tween 20 solution to soak for 5 minutes, rinse 5 times with sterile water, blot dry with filter paper, and transfer the petiole and receptacle of the virus-free Dahlia seedlings into adventitious bud induction medium (med...
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