Efficient expanding propagation method for dahlia toxin-free seedlings
A technology for dahlias and virus-free seedlings, applied in the field of agricultural science, can solve the problems of low seedling transplanting survival rate, low induction rate, low seedling proliferation efficiency, etc., and achieves improved transplanting survival rate, simple disinfection method and good disinfection effect. Effect
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[0046] The high-efficiency propagation method of dahlia virus-free seedlings in this embodiment was implemented in 2012-2013. The pre-treatment, plant regeneration and seedling hardening of dahlia virus-free seedlings were carried out in Lianyungang City Zhenxing Flower Garden, and the tissue-cultured seedlings were transplanted in It is carried out in the flower test base of Dongxin Farm in Lianyungang City. The detailed steps are as follows:
[0047] 1) Dahlia virus-free pre-seedling treatment:
[0048] Rinse the detoxified dahlia seedlings with tap water repeatedly first, wash once with a brush dipped in detergent, then rinse with ultrapure water, soak in 70% alcohol for 15 seconds, then rinse twice with sterile water, 0.1% mercury liter Add a few drops of Tween 20 solution to soak for 5 minutes, rinse 5 times with sterile water, blot dry with filter paper, and transfer the petiole and receptacle of the virus-free Dahlia seedlings into adventitious bud induction medium (med...
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