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Tissue culture and rapid propagation method of nobile-type dendrobium seedlings

A technology of tissue culture and dendrobium orchid, applied in the field of plant tissue culture, can solve the problems of large differences among spring dendrobium varieties, high operation technical requirements, easy contamination of explants, etc., to reduce seedling propagation costs, simplify disinfection methods, Overcoming the effect of low reproduction coefficient

Inactive Publication Date: 2014-09-17
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0007] However, due to the easy pollution of explants, high operating technical requirements, low reproduction rate, high cost, and large differences among the species of Dendrobium nodulum, there has been no report on large-scale production of Dendrobium orchid seedlings by tissue culture technology.

Method used

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  • Tissue culture and rapid propagation method of nobile-type dendrobium seedlings
  • Tissue culture and rapid propagation method of nobile-type dendrobium seedlings
  • Tissue culture and rapid propagation method of nobile-type dendrobium seedlings

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Embodiment 1

[0031] 1) Preparation and disinfection of explants: artificially pollinate Dendrobium orchids and bag them, and pick the capsules about 100 days later. On the ultra-clean workbench, disinfect the surface of the capsule with 75% (volume ratio) alcohol for 30-60 seconds, then soak it in 15-20% (volume ratio) sodium hypochlorite solution for 20 minutes, and rinse it with sterile water for 3 times.

[0032] 2) Induction culture of protocorms: dry the surface moisture of the sterilized Dendrobium capsules with sterile absorbent paper, cut the capsules with a scalpel, pick Dendrobium seeds, and inoculate them on the induction medium for dark culture ( The formula of induction culture medium is: MS+BA 0.6mg / L+NAA 0.1mg / L+hydrolyzed casein 0.5g / L+banana puree 40g / L+sucrose 30g / L+agar powder 6.0g / L, pH=5.4~5.6); After 20-30 days, the seeds of Dendrobium orchids began to germinate, and the culture was transferred to the light to continue to cultivate. After 40-50 days of light culture, ...

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Abstract

The invention discloses a tissue culture and rapid propagation method of nobile-type dendrobium seedlings. The method comprises the following steps of: (1) selection and disinfection of an explant; (2) induction culture of protocorm; (3) proliferation and differentiation of protocorm; (4) strong seedling and rooting culture; (5) transplanting of test-tube plantlets. According to the method, tissue culture and rapid propagation of the nobile-type dendrobium seedlings are carried out by the method provided by the invention, the culture procedure is simple, the explant is free of pollution, the propagation coefficient is large, the production cost is low, the seedlings are high in quality, and the defects of low propagation coefficient and the like in the prior art are overcome.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture rapid propagation method for spring dendrobium orchid seedlings. Background technique [0002] Dendrobium (Dendrobium) is a perennial flower of the Orchidaceae Dendrobium genus. Dendrobium is the largest genus in the Orchidaceae. Its origin is mainly distributed in tropical and subtropical Asia, Australia and Pacific Islands. There are more than 1,000 species in the world. There are about 76 species in my country, most of which are distributed in Southwest China, South China, Taiwan and other places. [0003] In horticulture, the varieties of Dendrobium orchids are generally divided into two categories: spring Dendrobium and autumn Dendrobium by their flowering period. Spring Dendrobium nobile (Dendrobium nobile) is a deciduous temperate species. Its flowers are brightly colored, luxurious and splendid, and are often planted as potted flow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 殷丽青汤桂钧周音李秀芬黄卫昌胡永红将建平刘炤胡海锋范晓芬
Owner SHANGHAI ACAD OF AGRI SCI
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