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A culture medium and culture method suitable for anthers and ovaries of weeping flax

A cultivation method and technology of flax anthers, applied in botany equipment and methods, horticultural methods, applications, etc., can solve problems such as capsule cracking, seed abortion, difficulty in directly applying new flax varieties, etc.

Active Publication Date: 2021-09-24
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overall, the haploid induction efficiency of flax is low, and it is difficult to be directly applied to the creation of flax germplasm resources and the breeding of new flax varieties
Although weeping flax has a long flowering period, it is difficult to obtain seeds due to the cracking of capsules, and the seeds are often aborted, so digging often occurs

Method used

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  • A culture medium and culture method suitable for anthers and ovaries of weeping flax
  • A culture medium and culture method suitable for anthers and ovaries of weeping flax
  • A culture medium and culture method suitable for anthers and ovaries of weeping flax

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This embodiment provides a kind of method that is used concurrently for anther and ovary culture to obtain the regeneration plant of weeping fruit flax, such as figure 1 shown, including the following steps:

[0047] (1) Selection of test genotypes

[0048] Healthy, disease-free weeping flax plants were selected as test materials.

[0049] (2) Selection of test materials

[0050] The test material grows in the wild without manual intervention. The flax with weeping fruit has a long flowering period. Generally, flower buds can be seen from May to August, and can be collected during the flowering period.

[0051] (3) bud picking

[0052] When picking buds, choose the buds on the flax plants that are strong and not infected by diseases and insect pests. Choose the buds with a bud length of 2-10mm at 9-10 am on a sunny day. Put it in a ziplock bag or a fresh-keeping bag, put it in an ice box, and bring it back to the laboratory. Care should be taken to avoid damage to...

Embodiment 2

[0068] The processing time of the 20% sodium hypochlorite that adopts in the weeping fruit flax anther culture process in embodiment 1 is through certain experimental research, and its process and result are as follows:

[0069] Continue to disinfect the surface of the alcohol-treated flower buds with 20% sodium hypochlorite for 2 minutes, 5 minutes, and 10 minutes respectively, and put them into a dark incubator for cultivation at a temperature of 22±2°C. When the callus grows to 2-3mm, then Transfer to a light intensity of 1500-2000Lx, 10h / d light, 14h / d dark, and culture at a temperature of 23-25°C. After one month of light culture, the callus induction rate and callus growth rate were counted. The results are shown in Table 1.

[0070] Table 120% sodium hypochlorite different treatment time on the anther callus induction of weeping flax

[0071]

[0072] It can be seen from Table 1 that surface disinfection treatment of weeping flax flower buds with 20% sodium hypochlo...

Embodiment 3

[0074] In this example, experiments were carried out on the effects of different concentrations of 2,4-D on the induction of callus of weeping flax. The process is as follows:

[0075] The explants after surface disinfection were inoculated in MS medium supplemented with different concentrations of 2,4-D for callus induction and proliferation. The culture conditions were: light intensity 1500-2000Lx, 10h / d light, 14h / d dark at a temperature of 22±2°C. One month later, the induction rate of callus and the growth of callus were counted, and the results are shown in Table 2.

[0076] Table 2 Effects of different concentrations of 2,4-D on callus induction of weeping flax

[0077]

[0078]

[0079] It can be seen from Table 2 that different concentrations of 2,4-D have a significant effect on the callus induction rate of weeping flax. Anthers and ovaries were inoculated in MS culture containing KT1.0mg / L and 2,4-D2.0mg / L Basically, the induction rates of anther and ovary ...

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Abstract

The invention discloses a culture medium and a culture method suitable for anthers and ovaries of weeping flax, belonging to the field of wild flax biotechnology. The culture method includes genotype selection, explant low temperature treatment, explant surface disinfection, callus induction, callus differentiation, adventitious bud elongation and rooting culture. The present invention uses the anthers and unfertilized ovaries of flax as materials to establish a haploid regeneration plant culture system suitable for flax and directly obtains haploid plants of flax, which are haploid of flax Sports breeding provides a certain reference value. At the same time, it has certain significance for the preservation of flax genetically improved germplasm resources and the selection of new varieties. It is also a means of preserving wild genetic resources and providing assistance for future scientific research.

Description

technical field [0001] The invention belongs to the technical field of wild flax, and in particular relates to a culture medium and a culture method suitable for anthers and ovaries of weeping flax. Background technique [0002] Weeping flax (L.nutans Maxim) belongs to the genus Flax of the family Linaceae, which belongs to the wild type and belongs to different species from the currently produced flax. Weeping flax is a perennial herbaceous plant with long flowering period, capsule dehiscence, unfulfilled seeds, and low germination rate. It mostly grows in sandy grasslands and dry hillsides, and has the advantages of strong stress resistance and high yield. Because weeping flax has excellent genetic material, it is the genetic source of important target genes in flax breeding and the basis for germplasm innovation and transformation. resource. [0003] Haploid breeding can greatly shorten the breeding period and speed up the breeding process, and has broad application pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丽丽李荣乔海明王玉祥李世芳曲志华杨绕华刘晶晶李爱荣马建富郭娜刘栋
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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