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Culture method of peony embryonic callus as well as culture medium

An embryogenic callus and culture method technology, applied in the field of plant tissue culture, achieves the effects of less environmental safety hazard, overcoming difficulties in inducing callus, and saving research costs

Inactive Publication Date: 2015-04-15
SHANGHAI BOTANICAL GARDEN +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liu Huichao et al. reported the establishment of callus induction and subculture system of tree peony, and studied the effects of different explants and hormones on callus induction and subculture proliferation, but there was no result of callus differentiation of tree peony.

Method used

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  • Culture method of peony embryonic callus as well as culture medium
  • Culture method of peony embryonic callus as well as culture medium
  • Culture method of peony embryonic callus as well as culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Induction and Culture of Embryogenic Callus of Jiangnan Tree Tree Variety (Fengdan) by Immature Embryos

[0036] 1) Prepare the culture medium for inducing embryogenic callus of tree peony according to the present invention, and the formula is shown in Table 2 below.

[0037] Table 2

[0038]

[0039]

[0040] After the culture medium is prepared, adjust the pH to 5.8-6.0, and sterilize at 121±2 degrees and 15-20 pounds for 20 minutes.

[0041] 2) collect the semi-mature seeds of peony about 90 days after pollination, wash them with running water, disinfect them with 70% (volume ratio) alcohol for 1 minute, then disinfect them with 15% (volume ratio) sodium hypochlorite solution (active chlorine content 5.2%) for 25 minutes, then rinse with sterile water.

[0042] 3) On the ultra-clean bench, cut peony seeds with a scalpel and peel off the embryos.

[0043] 4) seed embryos are inoculated in the medium prepared in step 1) and carried out dark culture, callus beg...

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Abstract

The invention discloses a culture method of peony embryonic callus as well as a culture medium. The peony embryonic callus is induced through adopting an MS culture medium which contains special components after modification (such as substances of 6-BA, KT cytokinin, TDZ plant growth regulators, PIC herbicide as well as casein hydrolysate); aiming at a peony embryo explant, the induction rate of the peony callus can achieve 100% by adopting the specific culture method provided by the invention, wherein the induction rate of embryonic callus can achieve 86% above.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for culturing embryogenic callus of peony and a culture medium thereof. Background technique [0002] Peony (Paeonia Suffruticosa) is a perennial shrub of Paeoniaceae and Paeoniae genus. It is a traditional famous flower in my country and has become one of the world's popular flowers and trees. The market prospect is very broad. However, due to the defects of low reproduction rate and long reproduction cycle in traditional peony propagation methods, the propagation and breeding process and commercial development of peony are seriously restricted. Propagation of peony by tissue culture is an inevitable trend of commercialization and industrialization of peony to solve the shortcomings of conventional propagation methods, and it is also an important basis for accelerating the pace of peony breeding and reproduction. [0003] At present, the following two approa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 胡永红殷丽青周音刘炤高清华张建军孙翊王新其
Owner SHANGHAI BOTANICAL GARDEN
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