30results about How to "Seedling time is short" patented technology

The invention provides a method for aseptic sowing and seedling raising of phalaenopsis and rhynchostylis retusa distant hybridization seeds. Phalaenopsis serves as a female parent, rhynchostylis retusa serves as a male parent, hybrid pods are obtained through artificial pollination, and distant hybrid progeny populations are obtained through the steps of pod pretreatment, aseptic sowing of seeds,seed germination, protocorm proliferation and differentiation, strong seedling rooting, acclimatization and transplantation and other steps. The method has the advantages of high seed germination rate, large seedling-forming number, short seedling-forming time, good seedling quality and the like, and the problems that distant hybridization seeds are not easy to successfully germinate due to affinity and are extremely difficult to germinate under natural conditions, and seedlings are not easy to grow are solved. Therefore, a large number of seedlings can be obtained in a short period through an aseptic sowing and artificial propagation method, and a foundation is laid for breeding of phalaenopsis distant hybridization excellent varieties.

The invention discloses a method for expanding propagation of aphidius gifuensis mummified aphids by utilizing water spinach. The method is characterized in that a tobacco moistening and seedling culture manner is adopted for planting the water spinach as a host plant, aphid inoculation and bee inoculation are respectively carried out in different growth periods of the water spinach for expanding propagation of the aphidius gifuensis mummified aphids, and multi-batch propagation expanding can be carried out during one sowing and planting. The method disclosed by the invention comprises the following concrete steps: a, preparing a propagation expanding chamber; b, sowing the water spinach and managing; c, inoculating aphids and breeding; d, inoculating the aphidius gifuensis, and breeding mummified aphids; e, collecting the aphidius gifuensis mummified aphids; and f, carrying out propagation expanding again. The method disclosed by the invention has the advantages that a flue-cured tobacco moistening and seedling culture manner is used for replacing tobacco as the host plant for breeding the aphidius gifuensis mummified aphids, the characteristics of the water spinach that the water spinach can be easily infected with aphids, a seedling formation period is short and the water spinach can be harvested for multiple times are utilized, short-period multi-batch propagation expanding of the aphidius gifuensis mummified aphids during one planting can be realized, propagation expanding amount is sufficient, propagation cost is low, and an existing tobacco seedling raising facility can be fully utilized, so that rational utilization of resource is realized.

The invention provides a cultivation method for hybridized all-female golden trout fries. An androgen is utilized to induce golden trout parent fish in a mature period so as to obtain golden trout pseudo-milter, steelhead parent fish eggs are fertilized with golden trout pseudo-milter sperms, hatching is performed, and the hybridized all-female golden trout fries are obtained. The eyed rate of the fertilized eggs during a hatching period is over 81%, the fry hatching rate is over 92%, the female rate of the obtained fries is 100% by detection, the rate of golden trout (with golden yellow body color) of the fries is over 90%, the weight gaining rate is over 122% after the fries are cultivated for 30 days, and the survival rate is over 98%. Compared with fries generated by purebred golden trout mating, the cultivation method for the hybridized all-female golden trout fries maintains the golden trout rate to be unchanged, enables the golden trout fries having double advantages of being hybridized and all-female to be obtained, and has good economic benefit and market value.

The invention discloses a paddy soilless transplant-free quick seedling raising method and equipment. The method comprises the following steps: a, preparing seedling raising nets, i.e., manufacturing square seedling raising nets by using a perishable and degradable biomass material, wherein the two ends of the seedling raising nets are fixed to fixing rods; b, transplanting seeds, i.e., transplanting the seeds in the seedling raising nets, wherein one ends of the seeds with embryos are exposed outside the net material and are in the same direction, and 2-3 seeds are transplanted for each plant; c, soaking to sprout, i.e., placing and soaking the seedling raising nets in which the seeds are transplanted in the previous step in a sprouting pool to sprout, and draining water in the pool and covering with thin films when the seeds are budded; and d, putting on the rack to raise seedlings, i.e., moving the seedling raising nets to an outdoor seedling raising rack when the seedlings are grown to 1-2 mm, covering with the thin films, watering once per hour, reserving holes in the thin films at the two ends of the seedling raising rack to ventilate when the seedlings are grown to 1.5 cm, removing the thin films to expose the seedlings in air when the seedlings in the seedling raising rack are grown to 8-10 cm, and transplanting into a paddy field after removing the thin film for 3-4 days.

The invention provides a method for cultivating grass plants, which comprises the following steps: (1) pretreatment of seeds; (2) germination of seeds; (3) transplanting strong seedlings with quartz sand; (4) cultivating in nutrient solution. Compared with the prior art, the present invention uses switchgrass seeds that have been naturally post-ripened for more than 2 years, and undergoes three stages of seed germination, transplanting strong seedlings with quartz sand, and cultivating seedlings with nutrient solution, and finally cultivates switchgrass seedlings. It has the advantages of simple equipment, easy operation, relatively shorter seedling time, and high consistency of specifications.

The invention discloses a tissue culture method for rapid growth of sweet cherry intraspecific hybrid F1 generation seedlings, which includes hybrid seed production and seed collection; the collected seeds are sequentially subjected to the following steps: S1, seed breaking treatment and seed embryo soaking; S2 1. Embryo disinfection and inner testa removal; S3, inoculation and cultivation; S4, inspection of embryo contamination and secondary disinfection; S5, browning treatment and timely transfer; S6, seedling hardening and transplanting. The present invention only uses a MS solid medium containing exogenous growth-promoting additives, the time required from germination to rooting is relatively short, the operation is simple and convenient, and the time for seedling formation of the hybrid F1 generation is greatly shortened, the entire breeding process is shortened, and sweetness is improved. Cherry breeding efficiency.

The invention discloses a method for directly regenerating hypocotyls and rooting in vitro, and relates to the field of plant biotechnology. The invention includes the following steps: (1) pretreatment of explants; (2) induction and subculture of adventitious buds Proliferation culture; (3) elongation culture of adventitious buds; (4) in vitro rooting treatment of elongated buds; (5) colonization and seedling cultivation of elongated buds. The beneficial effect of the present invention is that: the breeding process involved in the present invention has the advantages of high regeneration efficiency, simple operation, short seedling time, high seedling rate, etc., which lays the foundation for the large-scale breeding and variety improvement of later Qishu seedlings.

The invention provides a method for rapidly propagating tissue-cultured seedlings of Dendrobium chrysalis, which uses the mature capsule of Dendrobium chrysalis as the object of explants, designs special medium formulas for each cultivation stage, and achieves the purpose of rapid propagation through seeds-protocorms-buds The method comprises the following steps: selection and disinfection of explants, seed germination culture, strong seedling culture, rooting culture and test-tube seedling transplantation. The method of the present invention only needs 115d to 135d to obtain the Dendrobium chrysalis seedlings; the seed germination and bud differentiation of the present invention are carried out synchronously, which simplifies the cultivation process and reduces the cultivation cost, and the seed germination rate is high and the seedling time is short, i.e. The reproduction efficiency is high, thereby improving the efficiency of the whole seedling cultivation; in addition, the test-tube seedlings cultivated by the present invention are robust and have well-developed root systems, and through domestication and hardening, the environmental adaptability is strong, thereby improving the survival rate of seedling transplanting, Thereby realize the purpose of rapid breeding of Dendrobium chrysanthemum seedlings.

The invention provides a cultivating method of an oncorhynchus mykiss walbaum gynoecial hybrid offspring seed. The method comprises the following steps of: using male sex hormone to induce female oncorhynchus mykiss parent fishes in a sexual maturity stage to obtain oncorhynchus mykiss false milters with male functions; and fertilizing oncorhynchus mykiss walbaum eggs and oncorhynchus mykiss false milter sperms, and incubating to obtain gynoecial hybrid oncorhynchus mykiss walbaum offspring seeds. The eyeing rate of fertilized eggs in an incubating period is more than 83 percent, the offspring seed incubating rate reaches more than 90 percent, through test, the female ratio of the obtained seeds is 100 percent, the oncorhynchus mykiss walbaum ( golden yellow) rate of the offspring seeds reaches more than 91 percent, the weight gain rate of the offspring seed is more than 114 percent after 30-day cultivation, and the survival rate reaches more than 98 percent. Compared with an offspring seed produced by mating of a purebred oncorhynchus mykiss walbaum, according to the cultivating method of the oncorhynchus mykiss walbaum gynoecial hybrid offspring seed provided by the invention, the oncorhynchus mykiss walbaum rate remains unchanged, the oncorhynchus mykiss walbaum gynoecial hybrid offspring seeds with double advantages of hybridization and gynoecious are obtained, and better economic benefit and market value are achieved.

The invention provides a staghorn fern Platycerium wallichii The Hook. spore aseptic germination and seedling growth method uses staghorn fern leaves with mature spores as the explant material, designs special medium formulas for each culture stage, and achieves the purpose of rapid propagation through spore-GGB-buds, including the following steps: Selection and disinfection of explants, spore germination, GGB differentiation culture, rooting culture and transplanting of test-tube plantlets. The staghorn fern seedlings can be obtained in only 178d to 210d by adopting the method of the present invention; the spore germination of the present invention is carried out synchronously with the GGB multiplication, which simplifies the cultivation process and reduces the cultivation cost, and has a high spore germination rate, a high GGB multiplication rate, and a seedling The time is short, that is, the reproductive efficiency is high, and then the efficiency of the whole seedling cultivation is improved; in addition, the test-tube seedlings cultivated by the present invention are robust and have well-developed root systems, and through domestication and hardening, their environmental adaptability is strong, thereby improving the seedling transplantation. Planting survival rate, thereby realizing the purpose of rapid breeding of staghorn fern seedlings.

The invention discloses a method for rapidly propagating sprouts of chirita lutea by using chirita lutea leaves. Primary culture on explants, subculture and hardening and transplanting are carried out, and seedlings are directly obtained by adopting a two-step method including the primary culture and the subculture, a large number of strong seedlings can be obtained through propagation within about 90 days without a rooting phase, and the subculture is carried out without adding a hormone, and thus the production link is reduced, the production cost is lowered, and the effective propagation of the chirita lutea is realized. The method brings the convenience to collect materials without influencing the quantity of wild populations of the chirita lutea; the method is simple to operate, low in production cost, stable and efficient, and capable of greatly shortening the seedling growing period, and provides a technical support for protection and development and utilization of the chirita lutea.