61results about How to "Reducing sequence" patented technology

The present invention relates to the field of analysis of nucleic acid sequences. More specifically, the present invention relates to the method and instrument for high throughput parallel DNA sequencing. The present invention also provides method for selection of sequences from analyte samples for enrichment of the target sequences or depletion of the selected molecules and in particular undesirable sequence templates from sequencing samples.

The present invention relates to a random access process in a cellular communications system and to a user equipment (UE) and a NodeB adapted for performing the process. A problem with the random access is that the time slot for receiving a random access request (RA-request) has a long unused guard portion. When the UE transmits the RA-request, the distance to the receiving NodeB is unknown, and the purpose of the guard portion is to accommodate for propagation delay. The disadvantage is the inefficient use of the random access channel which results in long delays for UEs to access the network. The present invention solves the problem with a method in which the UE position is determined and the distance and propagation delay between the UE and NodeB is calculated before the transmission of the RA-request, and the timing of the transmission is advanced by the propagation delay.

The flexibility of a communication with a chatbot can be increased using a chatbot platform that can be integrated with a plurality of chat channels as well as facilitate communication between users of different chat channels. The platform can host chatbots that can leverage a plurality of resources, including internal and external natural language processors, machine learning, analytics services, and third party services to generate a response to user communications and take actions on behalf of the user. The use of the natural language processing and other additional information allows to generate an appropriate response to user queries, and to thus increase the speed with which user concerns are address. Further, the platform includes a chatbot creation program that allows a quick way to create a large number of customized chatbots without requiring advanced programming skills from the chatbot creator.

The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3′ side thereof, a sequence (A′) that can hybridize to a sequence (A) on the 3′ side of the target nucleic acid sequence. The second primer 1R includes, on the 3′ side thereof, a sequence (B′) that can hybridize to a sequence (B) on the 3′ side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5′ sides thereof, sequences (C) that are substantially identical to each other.

A speech recognition system includes an input device to receive voice sounds, one or more processors, and one or more storage devices storing parameters and program modules including instructions executable by the one or more processors. The instructions includes extracting an accoustic feature sequence from audio waveform data converted from the voice sounds encoding the acoustic feature sequence into a hidden vector sequence using an encoder network having encoder network parameters, predicting first output label sequence probabilities by feeding the hidden vector sequence to a decoder network having decoder network parameters, predicting second output label sequence probabilities by a connectionist temporal classification (CTC) module using CTC network parameters and the hidden vector sequence from the encoder network, and searching, using a label sequence search module, for an output label sequence having a highest sequence probability by combining the first and second output label sequence probabilities provided from the decoder network and the CTC module.

A method and apparatus are disclosed for improving the processing time of reduced complexity sequence estimation techniques, such as reduced state sequence estimation. Precomputing the branch metrics for all possible symbol combinations in the channel memory makes it possible to remove the branch metrics unit and decision-feedback unit from the feedback loop, thereby reducing the critical path. A set of multiplexers select the appropriate branch metrics based on the survivor symbols in the corresponding survivor path cells. The computational load of the precomputations is reduced for multi-dimensional trellis codes by precomputing each dimension of the multi-dimensional trellis code separately. A hybrid survivor memory architecture is also disclosed for a RSSE for a channel having a channel memory of length L, where the survivors corresponding to the L past decoding cycles are stored in a register exchange architecture (REA), and survivors corresponding to later decoding cycles are stored in a trace-back architecture (TBA) or REA.

In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an amino acid or peptide linker. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an amino acid or peptide linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including muliple sulfonate, alkylsulfonate, carboxylate and / or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention.

A method and apparatus are disclosed for improving the processing time of reduced complexity sequence estimation techniques, such as reduced state sequence estimation (RSSE). The possible values for the branch metrics in the RSSE are precomputed to permit pipelining and the shortening of the critical path. The computational load of the precomputations is reduced for multi-dimensional trellis codes by precomputing each dimension of the multi-dimensional trellis code separately. Prefiltering techniques are used to reduce the computational complexity by shortening the channel memory. A hybrid survivor memory architecture is disclosed for RSSE for a channel having a channel memory of length L, where the survivors corresponding to the L past decoding cycles are stored in a register exchange architecture, and survivors corresponding to later decoding cycles are stored in a trace-back architecture (TBA) or register exchange architecture (REA). Symbols are mapped to information bits to reduce the word size before being moved from the first register exchange architecture to the trace-back architecture (TBA) or the second register exchange architecture.

This invention relates to a method of encoding a path (1) in layered form so as to allow partial decoding and progressive rendering while decoding. The path (1) is made of an original sequence (20, 23) of original draw commands, each corresponding to an original portion of a drawing.The method of encoding comprises the following steps:transforming (E120, E230, E430, E440, E450) at least one original draw command into an approximating draw command according to a geometrical approximation of the corresponding original portion of a drawing, to obtain a reduced sequence (25);encoding (E130, E250) said reduced sequence (25) into an encoded reduced sequence; andencoding (E150, E260) a reconstruction information set (26) representing the difference between the reduced sequence (25) and the original sequence (20, 23).The invention is particularly suitable for graphics description languages, such as SVG.

The present invention provides a novel approach for storing, analyzing, and / or accessing biological data in a cloud computing environment. Sequence data generated by a particular sequencing device may be uploaded to the cloud computing environment during a sequencing run, which reduces the on-site storage needs for the sequence data. Analysis of the data may also be performed in the cloud computing environment, and the instructions for such analysis may be set at the originating sequencing device. The sequence data in the cloud computing environment may be shared according to permissions. Further, the sequence data may be modified or annotated by authorized secondary users.

An expert system, in particular for medical diagnosis, includes a memory 2 for storing a plurality of hypotheses. The hypotheses are arranged in a first and second disjoint group of hypotheses. The memory stores questions for rejecting hypotheses of the second group. An output 3 is used for supplying questions to a user. A input 4 is used for receiving initial data and answers to questions. A processor 5 is programmed to select questions from the stored questions for those hypotheses from the second group that are possible in dependence on the initial data. The processor also determines from answer(s) received in response to outputting the selected questions whether at least one of the hypotheses of the second group is possible. In response to determining that no hypothesis of the second group is possible, the processor supplies a most likely hypothesis of the first group.

Techniques for recovering reflectance and illumination from natural image sequences, which techniques can be implemented separately, or combined. The images in a sequence of images can be aligned. The images can also be transformed into the log opponent color domain to increase filter sparseness and reduce chrominance errors. Additionally, a collection of filters spanning frequency space can be employed that reduce low frequency artifacts.

Radiation damping (RD) is employed to hasten the recovery of longitudinal magnetization after RF excitation and signal readout in a magnetic resonance measurement cycle. A switch driven by the pulse sequence that performs the measurement cycle energizes a feedback RF coil driven by an amplified and phase shifted portion of the received MR signal. The recovery of longitudinal magnetization is thus under direct control of the MR system and enables the reduction of the otherwise inefficient waiting times that are required for natural T1 recovery of the excited spin magnetization. This enables shortened acquisition times, improved sensitivity, better spatial and temporal resolution, and reduction of motion artifacts that result from long acquisition times.

A first pulse sequence is executed on an imaging portion of an object to be examined using a multiple receiving coils including a plurality of receiving coils to obtain sensitivity images 701 to 703, each of n in number, which is smaller than the number of examination image. When those sensitivity images are calculated, NMR signals are measured only in a low-frequency region of a k space. Next, a second pulse sequence is executed while phase encoding steps are thinned out to acquire examination images 704 and 705, each of m in number (m>n), of the object with each receiving coil. When sensitivity distributions 707 and 708 are generated on the basis of sensitivity images 701 to 703, if a sensitivity distribution on a slice corresponding to examination images 704 and 705 is not yet measured, it is calculated with a slice interpolation processing on the basis of sensitivity distributions 701 to 703, and an aliasing artifact in examination images 704 and 705 are removed using sensitivity distributions 707 and 708 with a matrix calculation.

The present invention relates to a system for controlling the cutting of a first rip out of a first board and a second rip out of the first board or out of a second board. The system including a control module which provides instructions to stack and / or align the first board and the second board or the first rip and the second rip and instructions to process the first rip and the second rip simultaneously.

A method for generating a secret or a key in a network, the network including at least one first and one second member and a transmission channel between at least the first and the second members. The first and second members being able to place at least one first value and one second value on the transmission channel. The first member causes a first member value sequence and the second member causes a second member value sequence to be transmitted over the transmission channel largely synchronously with each other. The first member and the second member generate a shared secret or a shared key on the basis of information about the first member value sequence and the second member value sequence and on the basis of an overlap value sequence resulting from the overlap of the first member value sequence with the second member value sequence on the transmission channel.

The following invention outlines methodologies for the construction and utilization of transcription factor direct target gene microarrays of both DNA and corresponding protein / peptide target origin. The technology entails the array / microarray annotation and organization of transcription factor direct loci and corresponding protein products identified through modified and improved versions of chromosomal immunoprecipitation (CHIP) and molecular cloning procedures. It allows for the formulation of physiologically directed arrays which result in a thorough, focused characterization of the genetic and biochemical regulation occurring within a give population of cells or a given tissue. Arrays and microarrays of direct targets for any given transcription factor created utilizing this technology are substantially more clinically relevant for purposes of medical diagnostics and patient prognostics than conventional microarrays due to the physiologically focused nature and the transcription factor targets. In addition, the characterization and array organization of transcription factor target protein products and the assessment of their interactions with other proteins and / or small molecules is of critical importance for the purposes of understanding cellular and ultimately the design of therapeutics for human anomalies.

A technique is provided for animating an image or a portion of an image. In accordance with this technique, intermediary or transitional images, referred to as offset images, are displayed as part of an animation step to lessen abrupt changes in pixel values. In one embodiment, the offset images are generated using a weighted average of proximate pixels. In such an embodiment, the weight factor may take into account the distance of the offset from the proximate pixels such that closer pixels are more heavily weighted. Based on the direction of movement for the animation, the offset images are ordered and displayed as part of the animation steps of an animation sequence.

Systems (100) and methods for co-channel separation of communication signals. The methods involve: simultaneously receiving a plurality of communication signals transmitted at disparate relative Doppler frequencies from different locations within a multi-access system; performing matched filtering operations to pre-process each of the plurality of communication signals so as to generate pre-processed digitized samples using a priori information contained in pre-ambles (302, 304) of messages present within the plurality of communication signals; using estimated signal parameters to detect the plurality of communication signals from the pre-processed digitized samples; and demodulating the plurality of communication signals without using a Viterbi decoder.

The present invention provides a novel approach for storing, analyzing, and / or accessing biological data in a cloud computing environment. Sequence data generated by a particular sequencing device may be uploaded to the cloud computing environment during a sequencing run, which reduces the on-site storage needs for the sequence data. Analysis of the data may also be performed in the cloud computing environment, and the instructions for such analysis may be set at the originating sequencing device. The sequence data in the cloud computing environment may be shared according to permissions. Further, the sequence data may be modified or annotated by authorized secondary users.

A technique is disclosed for sample management for use in conjunction with sequencing devices that sequence biological samples, e.g., DNA and RNA. A sequencing device or a network of sequencing devices may be scheduled according to the characteristics of the samples in queue and the capabilities and availability of sequencing devices. Biological samples may be automatically queued and loaded via a sample distribution system. A sample distribution system may be used to reduce operator intervention.

Device-free localization for smart indoor environments within an indoor area covered by wireless networks is detected using active off-the-shelf-devices would be beneficial in a wide range of applications. By exploiting existing wireless communication signals and machine learning techniques in order to automatically detect entrance into the area, and track the location of a moving subject within the sensing area a low cost robust long-term tracking system can be established. A machine learning component is established to minimize the need for user annotation and overcome temporal instabilities via a semi-supervised framework. After establishing a robust base learner mapping wireless signals to different physical locations from a small amount of labeled data; during its lifetime, the learner automatically re-trains when the uncertainty level rises significantly. Additionally, an automatic change-point detection process is employed setting a query for updating the outdated model and the decision boundaries.

A Bluetooth responder device for transmitting non-Bluetooth related data is disclosed. The device (2) comprises means for receiving non-Bluetooth data to be sent, means for receiving an incoming Bluetooth inquiry packet, means for generating a Bluetooth FHS packet to be sent in response to the incoming Bluetooth inquiry packet, which FHS packet represents data received via the input; and means for sending the generated Bluetooth FHS packet. The non-Bluetooth data may be carried in an undefined field of the FHS packet.

The current methods and compositions provide for nucleotide sequences of promoters from Yarrowia lipolytica which may be used to drive gene expression in a cell. In some aspects, the promoters are useful for modulating lipid production in oleaginous organisms such as yeast.

Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) are disclosed herein. Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used to attach adapters and / or linkers to target RNAs. Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used in reactions, including, but not limited to, ligation reactions, amplification reactions, and sequencing reactions. Additionally, methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used for reducing and / or preventing the formation of secondary structures in target RNAs. These methods, compositions, and kits can also find use in a number of applications, for example, any application that benefits from stabilizing primary RNA structure, such as detecting and quantifying target RNAs in a sample, in the construction of small RNA libraries, in microarray and RT-qPCR applications, etc.

A first pulse sequence is executed on an imaging portion of an object to be examined using a multiple receiving coils including a plurality of receiving coils to obtain sensitivity images 701 to 703, each of n in number, which is smaller than the number of examination image. When those sensitivity images are calculated, NMR signals are measured only in a low-frequency region of a k space. Next, a second pulse sequence is executed while phase encoding steps are thinned out to acquire examination images 704 and 705, each of m in number (m>n), of the object with each receiving coil. When sensitivity distributions 707 and 708 are generated on the basis of sensitivity images 701 to 703, if a sensitivity distribution on a slice corresponding to examination images 704 and 705 is not yet measured, it is calculated with a slice interpolation processing on the basis of sensitivity distributions 701 to 703, and an aliasing artifact in examination images 704 and 705 are removed using sensitivity distributions 707 and 708 with a matrix calculation.

A sine-wave current line-start three-phase rare-earth permanent magnet synchronous motor includes a motor shaft, a rotor and a stator. The rotor is disposed inside the stator and rotates around the motor shaft. The rotor includes P pairs of poles. Each pole includes a polar arched surface. A planar surface is formed between adjacent polar arched surfaces. Thus, a non-uniform air gap is formed between the pole face of the rotor and the inner circumferential surface of the stator. By the use of a salient-pole structure with the non-uniform air gap, the asymmetry of the magnetic circuit caused by permanent magnets can be offset, thereby making the manufacturing process of the rotor simplified, the negative sequence components of the rotor current during the line starting process reduced significantly, the starting performance improved, the sine-wave current provided during the operating process, the harmonic loss reduced, and the motor efficiency enhanced.

Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA. The methods, compositions, systems and kits described herein may include, or include the use of, one or more single-stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.