The invention relates to a one-trip string two-layer layering perforation-layering testing combining tubular column and an operation method. The tubular column comprises a first oil tube, a depth correction short joint, a second oil tube, a circulating valve, a third oil tube, a testing valve
assembly, a second pressure gauge supporting cylinder, a second pressure transfer joint, a
straddle packer, a second
sieve tube, a spacer tube, a pressure guiding joint, a second ignition head, a second perforating gun, a
pressure release device, sealing layer well shut-in
sampling valve, a damping pressure gauge supporting cylinder, a first pressure transfer joint, an anchor packer, a first
sieve tube, a
shock absorber, a fourth oil tube, a first ignition head, a first perforating gun, a pressure relieving device, a second inner pressure transfer tube, a first pressure transfer tube and a first outer pressure transfer tube. The sealing layer well shut-in
sampling valve is located between the second perforating gun and the anchor packer. The damping pressure gauge supporting cylinder is located below the sealing layer well shut-in
sampling valve. by adopting the tubular column, two-layer layering perforation and layering testing combination can be completed, final well shut-in of the first layer does not need to occupy the operation
machine time independently, down-hole sampling is convenient, and the tubular column has the advantages of being short in period, low in cost and the like.