The invention discloses a method for preparing a 6,6'-bi(2,3-dimethoxyphenyl)-alpha,alpha-D-trehalose intermediate. The method comprises the following steps: (1) dissolving 2,3-dimethoxybenzoic acid in anhydrous tetrahydrofuran, adding N,N'-carbonyl diimidazole, stirring at room temperature, and performing carboxyl activation; (2) adding a compound shown as a formula II and N-bromo-succinimide in a catalytic amount into the reaction solution subjected to carboxyl activation in the step (1), stirring and reacting; and (3) adding water into the solution reacted in the step (2), stirring for 10-15 minutes, extracting for three times by using dichloromethane, mixing the organic layers, washing for three times by using a saturated potassium carbonate aqueous solution, washing by using saturated salt, drying by using anhydrous sodium sulfate, filtering, concentrating, recrystallizing the residues by using ethanol / water, thereby obtaining the 6,6'-bi(2,3-dimethoxyphenyl)-alpha,alpha-D-trehalose intermediate, removing the trimethylsilyl of the intermediate under weakly acidic conditions, thereby obtaining 6,6'-bi(2,3-dimethoxyphenyl)-alpha,alpha-D-trehalose. The method disclosed by the invention is mild in reaction conditions, low in cost and suitable for industrial production.