37results about How to "High flocculation rate" patented technology

The invention discloses a method for producing a microbial flocculant by utilizing culture waste water and residual activated sludge. The method comprises the steps of: first implanting bacterial strains into a seed culture medium to culture a seed liquid; second, adding cane sugar (as a supplemented carbon source) and phosphate (for adjusting pH) in the culture wastewater to prepare a culture wastewater fermentation medium, and mixing the culture wastewater with pretreated residual activated sludge in a volume ratio of 1:5-1:8 to prepare a culture wastewater-residual activated sludge fermentation medium; and at last, inoculating a 1-2% seed liquid to the fermentation medium, and using a two-step fermentation technology for fermentation production of flocculant, thereby producing the microbial flocculant. The method of the invention utilizes culture wastewater rich in N, P and K and residual activated sludge rich in organic matters to produce microbial flocculant, so as to greatly reduce the production cost and realize comprehensive utilization of waste. The production method is simple and easy, and the produced microbial flocculant has high flocculation performance.

The invention provides a pond culture seawater micro-plastic purification method, and belongs to the technical field of environmental pollution research. The method comprises the following steps: rapidly stirring a culture water body for 5-6 minutes at a speed of 200-300r / min; adding an aluminum salt coagulant and 4-hydroxy-3-methoxybenzyl alcohol into water, slowly stirring at 100-120r / min for 3-5min, adding a microbial flocculant into the water, continuously and slowly stirring for 6-8min, standing for 20-30min, and carrying out solid-liquid separation to obtain the aluminum salt coagulant,namely PAC. According to the pond culture seawater micro-plastic purification method provided by the invention, the collision probability of PAC and colloidal particles can be increased; the electricneutralization effect is improved, the stretching of a molecular chain of the microbial flocculant can be promoted, the floc growth is accelerated, and the floc particle size is increased, so that thefloc structure is more compact, the floc strength is enhanced, and the purification effect of the micro-plastic is improved.

The invention discloses thauera bacterial strain and application thereof and belongs to the field of environmental microorganism application. The bacterial strain is named as Thauera sp. CM-HZX2, the bacterial strain is preserved in China Center for Type Culture Collection (CCTCC) at present, the preservation number is CCTCC NO:M2015689, and the preservation data is November 2015. The thauera bacterial strain CM-HZX2 is high-efficient flocculating agent producing strain and can be widely applied to domestic wastewater, especially high-turbidity wastewater and a polluted water body of raw water. The thauera bacterial strain can grow best in conditions of pH of 7.0, temperature of 30 DEG C and rotation speed of 150r / min. After performing liquid shake culturing for 72h, the flocculation rate can reach up to 92.3%.

The invention discloses a compound microorganism bacterium agent sewage cleaning technology which belongs to the technical field of bioengineering. A great amount of contaminants are discharged into water bodies due to human activities, and as a result, the physical and chemical properties or biocoenosis compositions of the water bodies are changed, so that the use values of the water bodies are decreased. The severe consequence of water body pollution affects not only the health of human being but also industrial and agricultural production. According to the technology, efficient flocculation strain jelly bcereus, bacillus subtilis, aerobic bacillus licheniformis and nitrophilous actinomycete micromonospora are screened and adopted to prepare a compound microorganism bacterium agent (special for sewage). The compound microorganism bacterium agent has the advantages of small usage amount, high flocculation force, quick organic matter decomposition, no antagonism, good aerobic performance, no odor, no secondary pollution, pollution decomposition rate higher than pollution rate, high adaptability and viability, low cost and industrial production availability.

The invention discloses a preparation method of a microbial flocculant for purifying aquaculture seawater. The preparation method is characterized by carrying out synergistic fermentation on a mixed strain of klebsiella LDX1-1 and xanthomonas campestris CICC10258 with fermentation of a high-magnesium fermentation culture medium by adopting an antagonism-free compatible shake flask, and carrying out gelation strength treatment by adopting a salt mixture after the fermentation is completed. The preparation method disclosed by the invention is fast in fermentation speed, the technology can be easily operated, the fermentation concentration is high, and the production cost is low; the produced flocculant is used for treating high-salinity high-buoyancy seawater, has the characteristics of stable structure, flocculating block compactness and fast settling speed, and has an obvious effect in purifying the aquaculture seawater.

The invention relates to the field of microbiological science and in particular relates to a biological flocculation treatment method for coal preparation waste water. The method comprises the following steps: respectively preparing biological flocculants for coal from a Paenibacillus polymyxa culture solution and an Aspergillus niger culture solution, and respectively adding the biological flocculants into the coal preparation waste water for carrying out a flocculability test while controlling the test conditions. In combination with an infrared spectrum, chemical component analysis is performed on the biological flocculants for coal, thereby obtaining the result that both the biological flocculants for coal prepared from the Paenibacillus polymyxa culture solution and the Aspergillus niger culture solution have a good treatment effect on the coal preparation waste water.

The invention provides a method for preparing high-purity electronic grade strontium carbonate. The method comprises the following steps: dissolving strontium chloride into deionized water; adding mixture of strontium hydroxide accounting for 3 to 5 percent of the strontium chloride by weight and microbial flocculating agent MBFA9 into the deionized water; filtering the solution and removing impurities of the solution to be prepared into a strontium chloride with water for standby; slowly flowing a sodium hydroxide solution into the strontium chloride solution to react; pumping obtained strontium hydroxide solution into a carbonization reactor, and introducing pure carbon dioxide into the reactor to be carbonized to obtain strontium carbonate pulp; and drying and crushing the strontium carbonate pulp to obtain strontium carbonate powder. Compared with the prior process for producing the strontium carbonate, the method not only saves investment of high-temperature calcination equipment in production, but also greatly reduces energy consumption and pollution to environment, simplifies and optimizes production process, shortens reaction time, and ensures that the prepared strontium carbonate powder has the advantages of good dispersivity, high purity and the like.

The invention discloses a compound oil paint flocculating agent where bacterial cellulose shell-core structures are added. The following steps including the first step of strain separation and purification, the second step of strain screening, the third step of strain fermentation and cultivation and the fourth step of compound magnetic microsphere preparation are included. The preparation process is simple, the compound oil paint flocculating agent can be suitable for industrialized large-scale production, can be repeatedly used and can reduce cost, the problems that the flocculating efficiency is poor and sediment sinks in circulating water are effectively solved, and the long-term effective safe operation of the circulating water is ensured.

The invention relates to an application of citrobacter rodentium for circularly preparing biological flocculants by using waste algae, and belongs to a method for treating algal blooms. The application of the citrobacter rodentium for circularly preparing the biological flocculants by using the waste algae comprises the following steps of performing fermental cultivation on the citrobacter rodentium to obtain fermentation liquor under the condition that the inoculation amount accounts for 0.5-2% of the total weight of a fermentation medium; centrifugally removing mycelium to obtain liquid supernatant; adding ethyl alcohol of which the volume of the ethyl alcohol is twice greater than that of the liquid supernatant and standing for 12 hours at the temperature of 4 DEG C; centrifuging mixed liquid and dissolving obtained 3-5g of sediments in 100ml of deionized water; adding 2% of cetyl pyridinium chloride with the volume of 50ml in the deionized water and vibrating for 3-4 hours; collecting sediments and dissolving the sediments in 0.5M of NaCl liquor with the volume of 100ml; adding ethyl alcohol of which the volume is twice greater than the NaCl liquor; centrifuging and collecting sediments; eluting the collected sediments by using the ethyl alcohol and dissolving the sediments in 5ml of deionized water; and performing vacuum drying to obtain a product. The application of the citrobacter rodentium for circularly preparing the biological flocculants by using the waste algae has the advantages that the production cost of the biological flocculants is greatly reduced, and secondary pollution cannot be caused; and the citrobacter rodentium is easily degraded by microorganisms, is non-poisonous and harmful, is high in safety, and is beneficial to application and popularization of the biological flocculants.

The invention belongs to the technical field of sewage treatment, and particularly relates to a microbial flocculant as well as a preparation method and application thereof in sewage treatment. The microbial flocculant comprises a Raoultella XN-1 microbial strain, and the strain can be used as a microbial flocculant to remove heavy metals in wastewater, especially heavy metals Cr <6+>; the strain can also be directly used for culturing bacterial liquid to be used as a microbial flocculant, is easy to culture, is high in yield and has a wide application range.

The invention discloses a fermentation method for increasing the yield of polysaccharide as an active ingredient of biological flocclants, which relates to a fermentation method for increasing the active ingredient of the biological flocclants. The method comprises the following steps of firstly, inoculating flocculant-producing microorganisms into a flocculant fermentation fluid medium for fermentation to prepare seed solution; then inoculating the seed solution into the flocculant fermentation fluid medium which is added with exogenous AHLs for secondary fermentation to prepare the biological flocclants, so that the fermentation for increasing the yield of the polysaccharide as the active ingredient of the biological flocclants is finished; wherein the exogenous AHLs is one of N-3-oxygen-decoyl homoserine lactone and N- caproyl homoserine lactone or is a mixture of the same at any ratio. By adopting the method disclosed by the invention, the addition of the exogenous AHLs can improve the concentration and flocculation activity of the polysaccharide as the active ingredient of the biological flocclants, the flocculation effect of the biological flocculants is improved, and a basis is laid for large-scale industrial fermentation of the biological flocculants.

The invention belongs to the technical field of flocculant preparation. The invention discloses a method for preparing a flocculant by utilizing a fast-growing bacillus strain. The method includes thesteps of strain culture, fermentation culture, modified bentonite sedimentation, drying and the like, wherein the fast-growing bacillus strain is the fast-growing bacillus strain Celeribacter sp.GHF1031 obtained by separating and purifying sludge spit by ruditapes philippinarum. The flocculant prepared through the method is high in flocculation rate, stable in performance, free of toxic and sideeffects, high in safety, easy to produce and low in cost, and can be used as a wastewater flocculant.

The invention discloses a method for separating and filtering flocculating-demulsificating dual-functional strains and a culture medium that is used, relates to a strain separating and filtering method and a culture medium that is used, and solves the problems that existing oily sewage or oily sludge processing methods need two-stage processing of industrial strains that is divided into flocculating and demulsificating, has long process time, high energy consumption, high cost and more equipments, and two industrial strains cannot process oily sewage or oily sludge in high efficiency simultaneously in the same environment. The separating method comprises the following steps: 1. diluents with a plurality of proportions and different concentrations are prepared; 2. bacteria colonies are cultivated in a test tube; 3. individual bacterial colonies are selected and cultivated at constant temperature; and 4. separation and purification are implemented until the appearance of pure strains, and then the separation can be implemented. The separated and filtered flocculating-demulsificating dual-functional strains can simultaneously generate demulsificating and flocculating active ingredients, and are characterized by high demulsificating and flocculating rate. The culture medium that is used for filtering has strong directivity. The method for separating and filtering flocculating-demulsificating dual-functional strains has the advantages of short process time, saved energy, less cost and simple equipments.

The invention discloses an efficient compound water purifying agent and a preparation method thereof. The preparation method comprises the following steps: raw material pretreatment, seed powder degreasing, the crude extraction of flocculating active substances, the purification of the flocculating active substances, microbial flocculant preparation and compound water purifying agent preparation. The preparation method realizes the beneficial effects that raw materials are natural, a preparation process is simple and easy in implementation, added functional polypeptides can be used for enhancing the activity of moringa oleifera seed flocculating protein, the flocculation capability of the flocculating protein is enhanced, the flocculation rate of microbial flocculation and moringa oleifera seed flocculation active substance compound water purification is extremely high, the flocculation rate can be 95-98% when the use amount of the compound water purifying agent is 0.05-0.06g / L, and a finished product is safe and harmless compound water purifying agent product. In addition, the efficient compound water purifying agent is suitable for universal water bodies and has high economic value and environment protection value.

The invention discloses a preparation method of a microbial flocculant for purifying aquaculture seawater. The preparation method is characterized by carrying out synergistic fermentation on a mixed strain of klebsiella LDX1-1 and xanthomonas campestris CICC10258 with fermentation of a high-magnesium fermentation culture medium by adopting an antagonism-free compatible shake flask, and carrying out gelation strength treatment by adopting a salt mixture after the fermentation is completed. The preparation method disclosed by the invention is fast in fermentation speed, the technology can be easily operated, the fermentation concentration is high, and the production cost is low; the produced flocculant is used for treating high-salinity high-buoyancy seawater, has the characteristics of stable structure, flocculating block compactness and fast settling speed, and has an obvious effect in purifying the aquaculture seawater.

The invention provides a method for purifying microplastics in pond cultured seawater, which belongs to the technical field of environmental pollution research, comprising rapidly stirring the cultured water body at 200-300 r / min for 5-6 minutes, adding aluminum salt coagulant and 4-hydroxy-3-formazan to the water Oxybenzyl alcohol, stirring slowly at 100-120r / min for 3-5min, adding microbial flocculant to the water, continuing to stir slowly for 6-8min, standing for 20-30min, solid-liquid separation, the aluminum salt coagulant used is PAC. The method for purifying microplastics in pond cultured seawater can increase the collision probability between PAC and colloidal particles, improve the effect of electrical neutralization, promote the stretching of the molecular chain of microbial flocculants, accelerate the growth of flocs, and increase the particle size of flocs. It makes the floc structure more compact and enhances the strength of the floc, thereby improving the purification effect of microplastics.

The invention provides a method for preparing high-purity electronic grade strontium carbonate. The method comprises the following steps: dissolving strontium chloride into deionized water; adding mixture of strontium hydroxide accounting for 3 to 5 percent of the strontium chloride by weight and microbial flocculating agent MBFA9 into the deionized water; filtering the solution and removing impurities of the solution to be prepared into a strontium chloride with water for standby; slowly flowing a sodium hydroxide solution into the strontium chloride solution to react; pumping obtained strontium hydroxide solution into a carbonization reactor, and introducing pure carbon dioxide into the reactor to be carbonized to obtain strontium carbonate pulp; and drying and crushing the strontium carbonate pulp to obtain strontium carbonate powder. Compared with the prior process for producing the strontium carbonate, the method not only saves investment of high-temperature calcination equipment in production, but also greatly reduces energy consumption and pollution to environment, simplifies and optimizes production process, shortens reaction time, and ensures that the prepared strontium carbonate powder has the advantages of good dispersivity, high purity and the like.

The invention relates to a composite bacterial coal biological flocculant which has the advantages of high flocculation rate, reduction in the limit of various factors on a flocculation effect and wider application range. The composite bacterial coal biological flocculant comprises aspergillus niger bacterial solution and phanerochaete chrysosporium bacterial solution at a volume ratio of 1 : 1, wherein the aspergillus niger bacterial solution and the phanerochaete chrysosporium bacterial solution respectively refer to culture stock solution obtained by culturing aspergillus niger and phanerochaete chrysosporium, the centrifugal supernate of the culture stock solution obtained by centrifuging the culture stock solution, broken solution obtained by breaking the culture stock solution or broken centrifugal supernate obtained by centrifuging the broken solution. The culture stock solution of the aspergillus niger and the culture stock solution of the phanerochaete chrysosporium are obtained by the following steps respectively: a, enrichment culture, b, adaptability culture, c, further enrichment culture, and d, activity culture.

The invention belongs to the technical field of flocculant preparation. The invention discloses a method for preparing a flocculant by utilizing a fast-growing bacillus strain. The method includes thesteps of strain culture, fermentation culture, modified bentonite sedimentation, drying and the like, wherein the fast-growing bacillus strain is the fast-growing bacillus strain Celeribacter sp.GHF1031 obtained by separating and purifying sludge spit by ruditapes philippinarum. The flocculant prepared through the method is high in flocculation rate, stable in performance, free of toxic and sideeffects, high in safety, easy to produce and low in cost, and can be used as a wastewater flocculant.

The invention belongs to the technical field of flocculating agent preparation, and discloses a method for utilizing a white rhodobacteraceae strain to prepare a flocculating agent. The method comprises the steps of strain culture, fermentation culture, sedimentation of modified bentonite, drying and the like, wherein the white rhodobacteraceae strain is Albirhodobacter sp.GHF1032 which is obtained through separation and purification from sludge spit out from philippinarum ruditapes. The flocculating agent prepared by adopting the method has the characteristic of high flocculation rate, meanwhile, is stable in property, free of toxic and side effects, high in safety, easy to produce and low in cost, and can serve as a waste water flocculating agent.

The invention relates to the field of microalgae, in particular to a microalgae collecting method and a microalgae. The microalgae collecting method comprises the following steps of energizing microalgae culture liquid by an inert electrode, electrically shocking microalgae cells, and adding an inorganic flocculant to flocculate. The microalgae collecting method has the advantages that the usage amount of the fluocculant is greatly reduced, the flocculating time is greatly shortened, the flocculating rate of the microalgae is high, and the effects of being more economical and efficient are realized; especially for the microalgae with higher activity capability, such as flagellated microalgae, the effect is more outstanding.

The invention provides a method for separating lactic acid from fermentation liquor by flocculation and carrying out flocculate comprehensive utilization, belonging to the technical filed of industrial microbiology fermentation engineering. In the method, a pre-treatment is carried out before fermentation liquor separation by flocculation, and then obtained flocculate is returned to a fermentation tank and fermented with organic waste to produce lactic acid. The method comprises the following processing steps: 1) adding ammonia water or sodium hydroxide in the fermentation liquor for alkalization and heating the fermentation liquor; 2) adding a flocculant in the fermentation liquor, stirring until visible flocculate appears, and standing for obvious layering; and 3) separating solid from the liquor, and allowing the liquid phase to enter subsequent membrane separation lactic acid purification workstage and the solid phase to return to the fermentation tank to ferment with organic waste so as to improve lactic acid yield. The invention has the advantages of simple operation, high flocculation rate, easy separation of solid from liquor, low cost and little loss to target product of lactic acid, realizes flocculate comprehensive utilization, and reduces cost of flocculation pre-treatment of fermentation liquor.

The invention relates to a circulating slurry-water separation system for seabed ore collection. At least one vertical shaft is arranged below a hollow cavity, the rear side of the vertical shaft is provided with a vertical shaft outlet for ore to flow out, and the lower surface of the vertical shaft outlet is inclined downwards; a porous baffle inclining downwards is arranged in the vertical shaft and connected with the lower surface of the vertical shaft outlet, and the inclination trend is the same as that of the lower surface of the vertical shaft outlet; a vertical shaft inlet is formed in the bottom of the vertical shaft, is a low-speed water flow inlet and is connected with an outlet of a slurry-water separator through a flow-adjustable valve; and an outlet of a slurry separator isconnected with an inlet of the slurry-water separator through a submersible pump, and an outlet of the slurry-water separator is also connected with an inlet of an ore collecting head. According to the system, minerals, sea mud and water can be thoroughly separated, rapid sedimentation of the sea mud can also be achieved, the influence on the seabed environment is effectively reduced, meanwhile, the residual energy of water flow is utilized, and the separation rate of a slurry-water mixture is further increased.

The invention discloses a membrane electroflocculation device and a method for microalgae harvesting by utilizing the same. An anode area is formed by bipolar membranes, a positive electrode is placed in the anode area, cathode membranes and negative electrodes are connected to form cathodes, the cathodes are arranged around the anode area at a certain interval, and space formed between the cathodes serves as acid chambers; an algae pool is taken as a cathode area, the membrane electroflocculation device allows positive and negative ions to selectively penetrate, the memberane electroflocculation device is placed in algae liquid, calcium and magnesium ions in the algae liquid form a flocculatant under action of electric field force, and the algae liquid is enabled to be in an alkali-induced flocculation reaction, so that flocculation rate of the algae liquid is increased greatly. By the membrane electroflocculation device, flocculation rate can be ensured to be larger than 90% while concentration ratio is larger than 15 times after 2 hours when hydrolysis is finished. By the membrane electroflocculation device, acid liquid containing nutrient mineral elements in a culture medium substrate can be synthesized in the acid chamber, the mineral elements adhered in algae mud can be recycled by utilizing the acid liquid to clean the algae mud, and repeated culturing of microalgae can be realized.

The invention relates to the field of sewage treatment, and discloses an efficient microbial composite flocculant and a preparation method thereof. The efficient microbial composite flocculant is prepared from 1-10 parts of modified nano ferroferric oxide and 100 parts of mixed bacterium liquid of closterium and klebsiella; the modified nano ferroferric oxide is prepared by sequentially carrying out oleic acid modification and cation modification on nano ferroferric oxide. The nano ferroferric oxide subjected to two-step modification and a mixed bacterial solution of the closterium and the klebsiella are subjected to co-culturing, so that microorganisms and secretions are in full contact with ferroferric oxide particles, and the efficient microbial composite flocculant is formed. The efficient microbial composite flocculant has the advantages of high magnetism, high hydrophilicity, high stability, remarkable and stable flocculation effect, simple preparation process, mild reaction conditions, energy conservation, environmental protection, easiness in industrialization and the like, and can be used for various sewage treatments.

The invention belongs to the technical field of flocculant preparation. The invention discloses a method for preparing a flocculant by using Rhodobacter albicans strain, which comprises the steps of bacterial culture, fermentation culture, sedimentation of modified bentonite, drying and the like, wherein the Rhodobacter albicans strain is sludge spit out from Philippine clams Albirhodobacter sp. GHF1032, which was isolated and purified from R. albicans. The flocculant prepared by the method of the present invention has the characteristics of high flocculation rate, stable performance, no toxic and side effects, high safety, simple production and low cost, and can be used as a waste water flocculant.

The invention discloses a micro-electrolysis intensifying treater and a method for treating wastewater by using the same. The micro-electrolysis intensifying treater comprises a micro-electrolysis reactor, a micro-electrolysis iron carbon filler and a permanent magnet, wherein the micro-electrolysis iron carbon filler is adsorbed on the periphery of the permanent magnet to form a micro-electrolysis intensifying unit; the micro-electrolysis intensifying unit is arranged inside the micro-electrolysis reactor. According to the embodiment of the invention, the micro-electrolysis intensifying unit is formed by adding innumerable permanent magnets inside the micro-electrolysis treater, so that innumerable magnetic fields which are uniformly distributed can be produced, electrons orderly move and repeatedly move, long-chain substances and circular-chain substances are thoroughly decomposed, and the treatment effect of the micro-electrolysis wastewater is improved.

The invention relates to the technical field of sewage treatment, in particular to a high-efficiency flocculating bacterium and application thereof in sewage treatment, and the flocculating bacterium is pseudomonas guguanensis, is named as Peseomonas guguanensis 2021XNI14, and is preserved in the China Center for Type Culture Collection (CCTCC). The flocculating bacterium can be used for preparing a microbial flocculant, is used for treating aquaculture sewage, village sewage, domestic sewage and industrial sewage, and has the advantages of high efficiency, high nitrogen removal rate and high flocculation rate. The flocculating bacterium is easy to screen, high in culture yield and easy to be used for preparing a microbial flocculant, and has a wide application prospect in the sewage treatment industry.

The invention belongs to the technical field of flocculant preparation, and discloses a method for preparing a high-efficiency flocculating agent by using compound microbial inoculum. The method comprises the steps of: strain cultivation, fermenting cultivation, sedimentation by using modified bentonite, drying and the like, wherein the compounding strain is obtained by compounding a fast-growingbacillus strain Celeribacter sp. GHF1031 and a white Rubrobacter strain Albirhodobacter sp. GHF1032 which are obtained through separation and purification of mud which is spit out by Ruditapes philippinarum. The flocculating agent which is obtained by using the corresponding method and the compound microbial inoculum which is prepared from the fast-growing bacillus strain Celeribacter sp. GHF1031and the white Rubrobacter strain Albirhodobacter sp. GHF1032 has the characteristics of a high flocculation rate, has stable performance, no toxic and side effects, high safety, simple production andlow cost at the same time, and can be used as a wastewater flocculating agent.