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Microbial flocculant as well as preparation method and application thereof

A microbial flocculant and microbial technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high preparation cost, large dosage, and high cost of microbial flocculants

Active Publication Date: 2021-06-11
HUATIAN NANJING ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there have been many breakthroughs in the research on microbial flocculants at home and abroad, the preparation cost of microbial flocculants is very high, especially in the preparation of large quantities of high-purity , In the process of flocculating microbial flocculants with strong flocculation activity, whether it is equipment, medicine or other consumption, the cost is very high, and the preparation process is complicated, the yield of microbial flocculants is low, and the amount used in applications is increased by various constraints. Increased the difficulty of batch production of microbial flocculants

Method used

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  • Microbial flocculant as well as preparation method and application thereof
  • Microbial flocculant as well as preparation method and application thereof
  • Microbial flocculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Screening and identification of flocculation microbial strains

[0020] 1. Experimental materials

[0021] The test material is the activated sludge of the No. 2 Sewage Plant in Ma'anshan City, Anhui Province;

[0022] 2. Medium

[0023] LB liquid medium: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L;

[0024] LB solid medium: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar 20g / L.

[0025] Screening of flocculating microbial strains: Collect activated sludge samples from the sludge of the No. 2 Sewage Plant in Ma'anshan City, Anhui Province, weigh 10g of sludge samples into a beaker filled with 100mL of sterile distilled water, and stir them evenly with a magnetic stirrer; then, The samples were sequentially divided into 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 Six gradient dilutions; take 0.2mL of the diluted sample and evenly coat LB medium, place the plate upside down in a constant temperature incubator at 30°C for 48 hours; pick 12 str...

Embodiment 2

[0035] Example 2: Optimization of culture conditions for flocculant-producing microbial strain XN-1

[0036] (1) Temperature: Use an inoculation loop to pick up the XN-1 strain stored on the LB slope into 5 mL of LB liquid medium, shake and culture at 30°C and 180 rpm for 24 hours, and transfer 50 μL of the bacterial liquid to fresh In 5mL LB medium, cultured at different temperature conditions of 25°C, 30°C, 35°C, and 37°C for 48 hours, respectively, and measured the flocculation rate of the strains; The flocculation rate is up to 92%.

[0037] (2) pH: Use an inoculation loop to pick up the XN-1 strain stored on the LB slope into 5 mL of LB liquid medium, shake and culture it at 180 rpm at 35°C for 24 hours, and transfer 50 μL of the bacterial liquid to fresh The pH is respectively in 5mL LB culture medium of 4,5,6,7,8,9, measure the growth situation of OD600 comparison bacterial strain by ultraviolet spectrophotometer, the result shows that in the pH range of 5-8, bacterial...

Embodiment 3

[0039] Example 3: Activity distribution and extraction of microbial flocculants

[0040] Inoculate 0.5mL of XN-1 bacteria solution into two Erlenmeyer flasks filled with 50mL of LB liquid medium, and culture at 30°C and 180rpm for 48h. Collect one of the bottles of fermentation broth, centrifuge at 12000r / min for 10min, separate the supernatant from the cells, wash the cells twice with distilled water, suspend the cells in distilled water equal to the volume of the supernatant, and place Shake on a shaker for several minutes to mix evenly to obtain a bacterial cell suspension. Taking LB medium as a blank control, add equal volumes of fermentation broth, supernatant, bacterial suspension and culture medium into the kaolin flocculation system to measure its flocculation effect. The results are shown in figure 2 . Both the fermentation broth and the supernatant showed high flocculation activity, and the flocculation activity of bacterial cells was low, so most of the flocculants...

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Abstract

The invention belongs to the technical field of sewage treatment, and particularly relates to a microbial flocculant as well as a preparation method and application thereof in sewage treatment. The microbial flocculant comprises a Raoultella XN-1 microbial strain, and the strain can be used as a microbial flocculant to remove heavy metals in wastewater, especially heavy metals Cr <6+>; the strain can also be directly used for culturing bacterial liquid to be used as a microbial flocculant, is easy to culture, is high in yield and has a wide application range.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a microbial flocculant, its preparation method and its application in sewage treatment. Background technique [0002] Microbial flocculants are organic polymers with flocculation activity produced by microbial fermentation and cultivation. Through the flocculation process, organic polymer substances such as suspended particles, cells, and colloidal solids in wastewater are removed. Compared with chemical flocculants, microbial flocculants are safe, non-toxic, non-secondary pollution, natural degradation, good treatment effect, wide source and other advantages in the water treatment process, which has attracted widespread attention. [0003] Although there have been many breakthroughs in the research on microbial flocculants at home and abroad, the preparation cost of microbial flocculants is very high, especially in the process of preparing a large amount of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01C02F101/22
CPCC12N1/20C12N1/02C02F3/34C02F2101/22
Inventor 崔倩倩骆守鹏陶文达
Owner HUATIAN NANJING ENG & TECH CORP MCC
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