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Pond culture seawater micro-plastic purification method

A technology for microplastics and aquaculture water, applied in the field of environmental pollution research, can solve problems such as marine biological hazards, achieve the effect of promoting adsorption bridging, promoting microplastic bonding and floc settlement, and improving flocculation rate

Active Publication Date: 2020-08-18
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microplastics that adsorb other toxic pollutants can be more harmful to marine life, which also poses great pressure and challenges to the marine environment

Method used

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  • Pond culture seawater micro-plastic purification method
  • Pond culture seawater micro-plastic purification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. A preparation method of microbial flocculant, comprising the following steps:

[0036] The bacterial strain used is marine Bacillus licheniformis DHS-40, and the medium used includes:

[0037] LB solid medium: peptone 10.0g, yeast powder 5.0g, NaCl 10.0g, agar powder 1.5-2.0%, distilled water 1000mL, pH value 7.0, sterilized by high temperature steam at 121°C for 20min.

[0038] Expanded medium: soybean oil 0.49%, soybean meal hydrolyzate 5.0g / L, potassium dihydrogen phosphate 0.2g / L, calcium chloride solution (7.5g / L) 26μL / L, manganese sulfate solution (2.0g / L) 260μL / L, ferrous sulfate solution (2.0g / L) 100μL / L.

[0039]Inoculate Bacillus licheniformis into the liquid medium for expanded culture, collect the bacteria by centrifugation, wash the bacteria repeatedly with 10mM Tris.HCl (pH 8.0) solution and redissolve them to form a cell suspension. , to obtain cell crushing products; at 0-2°C, add ammonium sulfate solid powder to the cell crushing products to form ...

Embodiment 2

[0046] When carrying out flocculation, no radhamone was added, and the rest were exactly the same as in Example 1.

Embodiment 3

[0048] No 4-hydroxy-3-methoxybenzyl alcohol was added during flocculation, and the rest was exactly the same as in Example 1.

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Abstract

The invention provides a pond culture seawater micro-plastic purification method, and belongs to the technical field of environmental pollution research. The method comprises the following steps: rapidly stirring a culture water body for 5-6 minutes at a speed of 200-300r / min; adding an aluminum salt coagulant and 4-hydroxy-3-methoxybenzyl alcohol into water, slowly stirring at 100-120r / min for 3-5min, adding a microbial flocculant into the water, continuously and slowly stirring for 6-8min, standing for 20-30min, and carrying out solid-liquid separation to obtain the aluminum salt coagulant,namely PAC. According to the pond culture seawater micro-plastic purification method provided by the invention, the collision probability of PAC and colloidal particles can be increased; the electricneutralization effect is improved, the stretching of a molecular chain of the microbial flocculant can be promoted, the floc growth is accelerated, and the floc particle size is increased, so that thefloc structure is more compact, the floc strength is enhanced, and the purification effect of the micro-plastic is improved.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution research, and in particular relates to a method for purifying seawater microplastics in pond culture. Background technique [0002] At present, human society has entered the "plastic age". The production and use of a large number of plastic products has led to a large amount of plastic waste entering the environment, spreading on land, lakes, coastlines, ocean surfaces, seabeds and even abysses. Plastic is difficult to degrade and may become one of the important markers in the human era of the earth. In the marine environment, plastic has been widely distributed around the world due to its low density, high buoyancy and strong persistence. About 80% of marine solid waste is plastic waste. At present, more than 100 million tons of plastic waste are buried in landfills around the world every year, and another 150 million tons enter the land and marine environment. According to rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14C02F103/20
CPCC02F1/5236C02F1/54C02F3/34C02F2103/20
Inventor 谭朋徐冬冬陈睿毅王立改
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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