A tissue cultured
seedling micrografting method for
acer palmatum 'orange dream' is disclosed. The method includes (1) a step of culturing a sterile stock, namely a step of
cutting an acer elegantulum
branch segment with sprouts, performing
tissue culture to obtain an acer elegantulum sterile
seedling, and
cutting the acer elegantulum sterile
seedling in an inclined matter to obtain the sterile stock, (2) a step of culturing a sterile scion, namely a step of
cutting an
acer palmatum 'orange dream'
branch segment with sprouts, performing
tissue culture to obtain an
acer palmatum 'orange dream' sterile seedling, and cutting the acer palmatum 'orange dream' sterile seedling in an inclined matter to obtain the sterile scion, (3) a step of tissue cultured seedling micrografting, namely a step of aligning the oblique section of the sterile stock and the oblique section of the sterile scion, fixing to obtain a micrografted seedling, inoculating the micrografted seedling to a medium and culturing, and (4) a step of seedling hardening and
transplanting, namely a step of transferring the micrografted seedling obtained in the step (3) into a
greenhouse, performing earlier-period seedling hardening, transferring into a seed
bed, performing seedling hardening, performing mist spray treatment, sterilizing, and
transplanting after the seedling hardening is completed. The method increases the
grafting efficiency, increases the reproductive output, is high in
survival rate and can be used for large-scale cultivation of small-size acer palmatum 'orange dream' seedlings.