Liquid fermentation culture method of Paecilomyces lilacinus

Abstract

The invention discloses a liquid fermentation culture method of Paecilomyces lilacinus, comprising: preparing Paecilomyces lilacinus spore suspension; preparing a liquid fermentation medium of Paecilomyces lilacinus; inoculating lavender in the liquid fermentation medium Paecilomyces spore suspension, fermentation culture; the composition of the liquid fermentation medium includes a carbon source and a nitrogen source; the carbon source is selected from any one of glucose, sucrose, maltose, soluble starch or corn flour or Various; the nitrogen source is selected from any one or more of sodium nitrate, ammonium sulfate, peptone or soybean flour. The present invention screens and compares the various process parameters of the fermentation culture of Paecilomyces lilacinus, including the composition and dosage of the culture medium, the initial pH value of the culture medium, the inoculum size of the spore suspension, the temperature and the rotating speed of the fermentation culture, etc. By optimizing, the most suitable process parameters for sporulation were obtained. The fermentation culture method of the invention has high spore yield and relatively low production cost, and is suitable for large-scale production of Paecilomyces lilacinus fungicide.

Description

technical field [0001] The invention relates to a method for liquid fermentation and cultivation of fungi, in particular to a method for liquid fermentation and cultivation of Paecilomyces lilacinus (P. lilacinus), and belongs to the field of liquid fermentation and cultivation of Paecilomyces lilacinus. Background technique [0002] Paecilomyces lilacinus (P.lilacinus) belongs to the class Deuteromycetes, Hyphomycetes, Hyphosporaceae, and Paecilomyces genus. It is widely distributed all over the world and has the advantages of high efficacy, wide host range, and easy cultivation. Paecilomyces lilacinus is currently one of the main natural enemy fungi for the control of plant parasitic nematodes, and it is of great research and development value. Paecilomyces lilacinus is an effective parasite for the eggs of root-knot nematode incognita and white cyst nematode, and the parasitic rate of eggs of root-knot nematode incognita is as high as 60%-70%. It has control effect on a ...

AI Technical Summary

Problems solved by technology

[0004] The existing Paecilomyces lilacinus fermented culture and research on fermentation technology are still very immature, and there are many problems that need to be solved urgently, for example, along with the composition of culture medium, carbon source, nitrogen source, C / N and humidity, Variation of strains induced by changes in light, temperature and other factors leads to faster growth of mycelium but a decrease in spore production, and its biocontrol effect also decreases

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