The invention provides a method for increasing the panicle yield of a cuttage mother plant of Pinus massoniana, which comprises the steps of selection of the mother plant, planting of the mother plant, pruning and shaping, and management and protection of the mother plant. The tissue-cultured seedlings are used as the mother plant, and the tissue-cultured seedlings of Pinus massoniana are initially planted in a seedling container equipped with a seedling substrate, and then managed after the initial planting, and then the mother plant is truncated, and the planting is carried out on the prepared seedbed and after planting Management, when the buds of the mother plant grow to 5-6 cm long, the mother plant will be pruned and shaped, and at the same time, five aspects of water management, nutrient management, soil management, disease and insect pest control and mother plant renewal will be carried out on the mother plant. Management and protection. The mother plant cultivated by the present invention has a high survival rate, luxuriant branches and leaves, strong stems, and very vigorous vegetative growth, which effectively prolongs the service life of the mother plant, greatly improves the yield, quality and activity of spikes, and improves the rooting rate and survival of cuttings. The efficiency and quality of seedlings have improved the breeding effect of masson pine asexual seedlings.