44results about "Non-animal cells" patented technology

Cell culture apparatus comprising at least two adjacent cell cultivation channels separated by a permeable or semipermeable membrane, wherein at least one channel, for the majority of its length, has a cross sectional area of no more than 1 mm², said channel being provided with entrance and exit means to permit the passage of media therethrough, allows co-culture of separate cell types, e.g. human and microbial cells, without mingling, allowing monitoring of cell cultures and chemical exchanges between the respective cell cultures.

The invention provides a fusobacterium nucleatum subsp.animalis strain THCT5A4 separated from human rectal cancer tumor tissue. A classification name is Fusobacterium nucleatum subsp.animalis THCT5A4,a preservation number of the fusobacterium nucleatum subsp.animalis strain THCT5A4 separated from the human rectal cancer tumor tissue is CCTCC NO: M 2019366, the preservation date is May 17th, 2019,the preservation organization is China General Microbiological Culture Collection Center, and a 16SrRNA gene sequence of the fusobacterium nucleatum subsp.animalis strain THCT5A4 separated from the human rectal cancer tumor tissue is shown in SEQ ID NO:1 as shown in the description. A separation method of the fusobacterium nucleatum subsp.animalis strain is provided. A result of co-culture of a colorectal cancer cell line and the THCT5A4 shows that the THCT5A4 can significantly promote proliferation of colorectal cancer cells, therefore, the THCT5A4 can provide diverse in-vitro or in-vivo experimental conditions for simulating an intestinal environment for studies of colorectal cancer, and a colorectal cancer disease model can be built for screening medicines for treating colorectal cancer.

The invention provides a fusobacterium nucleatum polymorphic subspecies isolate THCT15E1, a classification name is Fusobacterium nuucleates subsp.nucleatum THCT15E1, a preservation number of the strain is CCTCC NO: M 2019362, a preservation date is May 17, 2019, a preservation unit is China Center for Type Culture Collection, and the 16SrRNA gene sequence of the strain is shown as SEQ ID NO: 1. The result of co-culture of the colorectal cancer cell line and THCT15E1 shows that THCT15E1 can significantly promote colorectal cancer cell proliferation; therefore, the THCT15E1 can provide diversified experimental conditions for in-vitro or in-vivo intestinal environment simulation for colorectal cancer research, and can also construct a colorectal cancer disease model to screen drugs for treating colorectal cancer.

The invention discloses a method for greatly increasing the PCV2 cultivation virus titer through a streptococcus culture preparation, and belongs to the technical field of cell engineering and virus cultivation. The streptococcus culture preparation is prepared through high-temperature inactivation of streptococcus-containing supernate which is obtained after co-culture of streptococcus and anchorage-dependent cells in a maintenance culture medium. The streptococcus is streptococcus agalactiae, the cells are PK-15 cells, and the content of the streptococcus in the streptococcus-containing supernate is 100-900 million/mL. The streptococcus culture preparation is added into the maintenance culture medium according to the concentration of 0.1-1% to prepare a special culture medium used for stimulating PCV2 to generate viruses, the cultivation virus titer of the culture medium can be greatly increased by adopting the special culture medium as the maintenance culture medium for culturing PCV2, and the virus titer can reach 10<-7> or above; and a production process is greatly simplified, and the production cost is greatly lowered.

A method of co-culturing helicobacter pylori and gastric mucosa epithelial cells is disclosed. The method includes 1) preparing a helicobacter pylori medium; 2) adding the medium into a sterile cell culture flask and preparing a slant surface; 3) culturing gastric mucosa epithelial cells in the culture flask in which the slant surface is formed and allowing the cells to be adhered to the wall of the flask; 4) centrifuging a helicobacter pylori 48-h culture liquid, and then resuspending a centrifuging product with a cell culture liquid; and 5) adding a resuspended bacterial liquid into the culture flask in which the slant surface and the adherent cells are formed, culturing, and counting the living bacteria and observing cell morphological changes every day. A beneficial effect of the method is that the novel method of co-culturing the helicobacter pylori and the gastric mucosa epithelial cells is provided. In the novel system, the helicobacter pylori can grow normally and continuously stimulate the adherent cells to generate lesions, thus simulating a human gastric mucosa infection situation well. The problem that helicobacter pylori declines or dies and cells are not effectively stimulated because culture liquids are not suitable for growth of the helicobacter pylori in traditional methods in which helicobacter pylori are directly added is overcome.

The present invention relates to a method for the preparation of an immunogenic composition for the treatment and/or prophylaxis of mycoplasma infections in a subject comprising the cultivation of mycoplasma bacteria in a serum-reduced or swine serum-free, eukaryotic cell system; obtaining an antigen of the mycoplasma bacteria; and addition of a pharmaceutically acceptable carrier. Further, the present invention relates to the immunogenic composition obtainable by said method and a method for immunizing a subject comprising the administration of said immunogenic composition to a subject.

Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.

The invention relates to a preparation method and an application of a cell membrane coated nano topological structure array. The preparation method comprises the following steps: stimulating macrophages to form stimulated macrophages, and extracting stimulated macrophage membranes; meanwhile, processing the substrate to form a substrate with nanowires, and processing the substrate with the nanowires to form a nanowire substrate with positive charges; and combining the stimulated macrophage membrane with the nanowire substrate with the positive charges to obtain the macrophage membrane modifiednano topological structure array. The cell membrane coated nano topological structure array is simple in preparation and operation, and can be applied to capture of bacteria.

Hepatitis C virus replication at extrahepatic sites has been suggested; however, complete viral replication has only been confirmed in hepatocytes. Here we show that human adipogenic DLK-1⁺ stem cells (hADSC) freshly isolated from HCV-infected individuals contained viral transcripts, replication intermediates and viral antigens in vivo, and viral transcripts increased in supernatants upon prolonged ex vivo culture. Furthermore, naive hADSC isolated from HCV (−) individuals support complete replication of clinical isolates in vitro, and the infection is donor-nonspecific for cells and cross-genotypic for viruses. Viral infection/replication is mediated through CD81, LDL-R, SR-B1, EGFR, Apolipoprotein E, occludin, claudin-1, NPC1L1 and diacylglycerol acetyltransferase-1, and can be inhibited by anti-viral drugs. In addition, the physical properties of hADSC-propagated viral particles resemble clinical isolates more than JFH1/HCVcc, and viruses propagated by in vitro infected hADSC are infectious to primary human hepatocytes. Therefore, hADSC are an in vivo HCV reservoir and represent a novel venue of clinical virus-host interaction. hADSC can also be exploited as a physiologically relevant primary cell culture system to propagate clinical isolates.

Described is a method to transfer chromosomal DNA between two microbial species without genetic engineering or vectors. The strains resulting from this method are chimeric microbial hybrids that can express a combination of genotypes from both parents.

A culture container for culturing epithelial cells, includes an upper container, a lid member configured to airtightly fit with an opening portion of the upper container, and a lower container configured to accommodate the upper container and a cell culture medium, in which at least a part of an area of the upper container in contact with the cell culture medium is formed of a membrane that is permeable to at least a part of components of the cell culture medium and impermeable to a cell, and a material of the lid member has an oxygen permeability coefficient of 1.0×10⁻⁶ cm³ cm/(cm²·sec·atm) or less.

Herein is reported a method for co-cultivating B-cells in the presence of phorbol myristate acetate, IL-1beta, TNFalpha, IL-2, IL-10 and IL-6.