91 results about "Vaginal epithelial cell" patented technology

An effective treatment method for improving the appearance of epithelia, such as lip epithelia and vaginal epithelia is provided. According to the present method, an effective amount of a composition containing retinoid, preferably in a cosmetically acceptable carrier, is topically applied to the vaginal or lip epithelia. The present invention also includes compositions for practicing the method.

The invention belongs to the field of biomedicines and discloses a construction method and application of a human normal vaginal epithelium 3D (Three Dimensional) differentiation culture model. A 2D (Two Dimensional) growth culture medium is provided and normal vaginal epithelial cells separated and cultured from normal tissues beside female vaginal cancer are obtained; any exogenous gene is not introduced and the normal vaginal epithelial cells have a physiological function of normal differentiation. The method for constructing the human normal differentiation vaginal epithelium 3D model comprises the following steps: re-suspending single cells by the 2D culture medium and inoculating the single cells into a gas-liquid culture device; replacing the growth culture medium with a differentiation culture medium and culturing for 14 to 21 days; after completely differentiating 3D gas-liquid culture of the human vaginal epithelial cells, inoculating HSV-2 (Herpes Simplex Virus-2) virus liquid to obtain an HSV-2 virus infection 3D model. The two types of 3D models can be used for physiological studies and drug toxicity safety evaluation of human normal reproductive tract epithelium, researches of pathogenic mechanisms of HSV-2 virus infected diseases and sexual transmission pathogen infected diseases and researches and development of anti-viruses medicines.

The invention relates to the technical field of medicaments, in particular to application of 12 isoflavone compound monomers comprising belamcanda chinensis total isoflavone or tecoridin, wild tectoridin, tectorigenin, iridin and the like in preparing medicaments and food for preventing and treating lower women's estrogen level and retarding womb and ovarian failure or climacteric syndrome. Animal experiment results show that the total isoflavone or 12 isoflavone compound monomers in the belamcanda chinensis have remarkable pharmacological effects of improving the estrogen level, promoting the growth of follicle, promoting epitheliosis of womb and vagina, retarding womb and ovarian failure, improving the expression of an epithelial cell estrogen receptor of the vagina and the like, so the total isoflavone or 12 isoflavone compound monomers can be used for preparing the medicaments or food for preventing and treating lower women's estrogen level and retarding womb and ovarian failure or climacteric syndrome. The application provides a new source for seeking a plant estrogen medicament.

The invention relates to a probiotic composition for nursing a female reproductive system. The probiotic composition is prepared from the following raw material components: lactobacillus vaginalis, lactobacillus salivarius, lactobacillus paracasei, lactobacillus acidophilus, lactobacillus reuteri, lactobacillus iners, lactobacillus crispatus and chitosan. The components are appropriately proportioned by weight to finally obtain powder having the effects of inhibiting growth of pathogenic microorganisms, relieving and treating vaginitis, restoring the self-cleaning function of the female reproductive system and improving the immunity function; the probiotic composition prevents the pathogenic microorganisms from adhering to vaginal epithelial cells through a competitive adhesion mechanism and stimulates the immune system to maintain vaginal microecological balance. The probiotic composition has the advantages that infections of pathogenic bacteria are inhibited through active probiotics, the self-treating and self-healing effect can be achieved, the restoration of a microecological balance state is promoted, and the problems of the female reproductive system are solved from the source.

The invention provides a strain. The strain is a Lactobacillus gasseri strain, and the preservation number of the strain is CGMCC No.6361. The invention also provides a use of the strain in the preparation of colpitis prevention and / or treatment drugs, a use of the strain in the preparation of vagina nursing articles, and a use of the strain in the preparation of external genitals health articles. By adopting the above technical scheme, the strain can effectively realize an antagonistic effect on a plurality of pathogenic bacteria. The strain has a high adherence ability to vaginal epithelial cell. The strain can well meet practical clinic application demands.

The invention relates to a caper extract, a caper cream and a production method thereof. The caper extract contains 1.2-2.0 percent total alkaloids according to the weight percentage. Raw materials of the caper cream comprise caper, stearic acid, glycerin monostearate, white Vaseline, glycerin, sodium dodecyl sulfate, laurocapram and ethyl p-hydroxybenzaote. The pharmacodynamic test results show that the caper cream can significantly promote the formation of epidermal granular layer in the scales of mouse-tail., significantly inhibit the mitosis of mouse vaginal epithelial cells, reduce the dextran-induced mouse itching times and the itching sustained total time, inhibit the xylene-induced mouse auricula edema and the acetic acid-induced mouse peritoneal capillary permeability increase and promote the generation of mouse hemolysin antibody. The preparation is prompted to have better therapeutic effect for mouse scleroderma and psoriasis. The caper cream has good transdermal absorption, durable action, no irritation and easy smearing, washing and removal by patients.

The invention discloses a culturing method of combined sequential cell in the culturing technique scale of human vaginal epithelial cell, which is characterized by the following: adopting non-serum culture medium and serum culture medium to do sequential culture; breeding human vaginal epithelial cell rapidly; improving successful generation rate at 80%.

The invention discloses a Lactobacillus crispatus 262-1 and its application in gynecological diseases. The Lactobacillus crispatus 262-1 is a new Lactobacillus crispatus strain, and has a preservation number of CGMCCNo.6469 in China General Microbiological Culture Collection Center. The Lactobacillus crispatus 262-1 has the advantages of strong acid and H2O2 production ability, strong adherence to vaginal epithelial cells, substantial resistance on bacterial vaginal diseases and various vaginal gynecological diseases, safety, non-toxicity, good stability and long term preservation. The invention also relates to a use of the Lactobacillus crispatus 262-1 in medicines for preventing and / or treating the gynecological diseases.

The invention provides a drug composition for treating senile vaginitis and a preparation method thereof. The drug composition takes estriol and moxifloxacin hydrochloride as the main active ingredients, is mainly used for treating such symptoms of senile vaginitis as vaginal wall atrophy, mucosa thinning, local resistance decrease and inflammations caused by bacteria invasion and reproduction and has the functions of recovering the activity of the vaginal epithelial cell and suppressing growth and reproduction of bacteria in the vagina. The drug composition is used for preferably preparing such dosage forms for intravaginal administration as suppositories, gels and ointments.

The present invention addresses the problem of providing a new therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The presentinvention is a stratified squamous epithelial cell normal differentiation and maturation promoting agent that comprises secretions of mesenchymal stem cells. It is preferable that the secretions notinclude animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and thatthe stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells,epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.