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Lactobacillus jensenii and application thereof

A technology of Lactobacillus johnsonii and bacterial agent, applied in the field of microorganisms, can solve problems such as easy recurrence, and achieve the effect of strong adhesion

Active Publication Date: 2015-01-21
北京益生苑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Urogenital tract infection is a common and frequently-occurring disease in women, and is prone to recurrence. Although antibiotics have a good short-term effect, there is a 40% recurrence rate

Method used

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  • Lactobacillus jensenii and application thereof
  • Lactobacillus jensenii and application thereof
  • Lactobacillus jensenii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Isolation and identification of Lactobacillus jannaschii strains

[0027] 1. Isolation of Lactobacillus jannaschii strains

[0028] More than 200 samples of vaginal secretions from healthy women who were clinically detected without symptoms and signs of vaginitis were serially numbered and inoculated into MRS liquid medium and cultured anaerobicly at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Hundreds of isolated bacteria were tested for strain identification, hydrogen peroxide production, and adhesion to vaginal epithelial cells, and one strain of Lactobacillus jannaschii with strong adhesion and hydrogen peroxide production was named It is IMJ-19.

[0029] 2. Identification of Lactobacillus jannaschii strains

[0030] (1) Morphological identification

[0031] The strain IMJ-19 isolated in the above step 1 was inoculated on the MRS Lact...

Embodiment 2

[0043] Example 2. Functional analysis of Lactobacillus jannaschii IMJ-19

[0044] 1. Lactobacillus jannaschii IMJ-19 produces H 2 O 2 Detection

[0045] (1) H 2 O 2 The preparation of the standard curve: First, use a 100mmol / L piperazine-N,N'-bis-ethanesulfonic acid (Piperazine-N,N-Bis 2-ethanesulfonic acid, PIPES) aqueous solution to set the volume percentage to 30 % H 2 O 2 Aqueous solution (equivalent to 9.128mol / L) stock solution is diluted to 1mol / L, and then 100mmol / L PIPES aqueous solution to the above 1mol / L H 2 O 2 Dilute to 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L respectively, and mark the above dilutions and 100mmol / L PIPES aqueous solution as H 2 O 2 Working solution; then take 100μL of the above H 2 O 2 The working solution was mixed with 100μL of TMB (tetramethylbenzidine) aqueous solution with a concentration of 20mmol / L, 2μL of horseradish peroxidase (purchased from TaKaRa) with a concentration of 1mg / mL, incubated at 16°C for 10min, and measured OD 630 val...

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Abstract

The invention discloses a lactobacillus jensenii and application thereof. The lactobacillus jensenii disclosed by the invention is named as IMJ-19, and is collected in the China General Microbiological Culture Collection Center on July 14th, 2014, with the collection number of CGMCC No.9439. The lactobacillus jensenii IMJ-19 has strong capability of generating H2O2, can effectively inhibit the growth of foreign pathogenic bacteria and other conditioned pathogens, also has strong adhesion action to vagina epithelial cells, and ensures the colonization of strains on vaginal epithelial, and the lactobacillus jensenii IMJ-19 has certain application prospects on the aspect of prevention and treatment of urogenital tract infection.

Description

Technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Lactobacillus jannaschii and its application. Background technique [0002] Genitourinary tract infection is the most common but under-appreciated frequently-occurring disease in women. The incidence is second only to respiratory tract infection, which can be as high as 40%. Female genitourinary tract infection is the general term for urinary tract infection and reproductive tract infection. Women's physical and mental health will also affect the happiness and stability of many families, and in turn affect the population quality and economic development of the entire society. The common pathogen of female urinary tract infections is gram-negative bacilli, among which Escherichia coli is the most common, followed by staphylococcus; most of these are the normal flora of the human intestine and skin mucosa. Due to the decline of the body’s immunity, these Bacteria can migrate t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/00C12R1/225
CPCA61K35/747C12N1/205C12R2001/225
Inventor 朱宝利张瑞芬李晶王江
Owner 北京益生苑生物科技有限公司
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