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Lactobacillus crispatus and application thereof

A kind of Lactobacillus campylobacter, bacterial agent technology, applied in the field of microorganisms, can solve problems such as easy recurrence, and achieve the effect of strong adhesion

Active Publication Date: 2014-12-24
北京益生苑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Urogenital tract infection is a common and frequently-occurring disease among women, and is prone to recurrence. Although antibiotics have a good short-term effect, there is a 40% recurrence rate

Method used

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  • Lactobacillus crispatus and application thereof
  • Lactobacillus crispatus and application thereof
  • Lactobacillus crispatus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, isolation and identification of Lactobacillus flexus strain

[0031] 1. Isolation of Lactobacillus flexus strains

[0032] The vaginal secretion samples collected from more than 200 cases of healthy women without symptoms and signs of vaginitis were numbered sequentially, inoculated into MRS liquid medium, and incubated anaerobically at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Hundreds of isolated bacteria were tested for species identification, hydrogen peroxide production and adhesion to vaginal epithelial cells, and one strain of Lactobacillus flexures with strong adhesion and hydrogen peroxide production was named IMC-12.

[0033] 2. Identification of Lactobacillus flexus strains

[0034] (1) Morphological identification

[0035] On the MRS Lactobacillus medium plate, the bacterial strain IMC-12 obtained in the above...

Embodiment 2

[0048] Example 2, functional analysis of Lactobacillus crispatus IMC-12

[0049] 1. Production of H by Lactobacillus crispatus IMC-12 2 o 2 detection

[0050] (1) H 2 o 2 The making of standard curve: first use the aqueous solution of piperazine-N, N'-di-ethanesulfonic acid (Piperazine-N, N-Bis 2-ethanesulfonic acid, PIPES) with concentration to be 100mmol / L to make the volume percentage content be 30 %H 2 o 2 Aqueous solution (equivalent to 9.128mol / L) stock solution is diluted to 1mol / L, and then the above 1mol / L H with 100mmol / LPIPES aqueous solution 2 o 2 Dilute to 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L respectively, and record the above dilutions and 100mmol / L PIPES aqueous solution as H 2 o 2 working solution; then take 100 μL of the above H 2 o 2 The working solution was mixed with 100 μL of 20 mmol / L TMB (tetramethylbenzidine) aqueous solution and 2 μL of 1 mg / mL horseradish peroxidase (purchased from TaKaRa Company), incubated at 16 °C for 10 min...

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Abstract

The invention discloses lactobacillus crispatus and application thereof. The perseveration number of the lactobacillus crispatus is as follows: CGMCC No.9388. The lactobacillus crispatus IMC-12 disclosed by the invention has relatively strong capacity of producing H2O2 and can be used for effectively inhibiting the growth of external pathogenic bacteria and other conditioned pathogenic bacteria and meanwhile, the lactobacillus cripatus has relatively strong adhesive attraction for vagina epithelial cells, so that the field planting of the vagina epithelial cell is guaranteed, and thus, the lactobacillus crispatus IMC-12 has certain application prospect in preventing and treating urogenital tract infection.

Description

technical field [0001] The invention relates to a strain of lactobacillus flexus and application thereof, belonging to the field of microorganisms. Background technique [0002] Urogenital tract infection is the most common but not paid attention to frequently-occurring disease in women, and the incidence rate is second only to respiratory tract infection, which can be as high as 40%. Female genitourinary tract infection is a general term for urinary tract infection and reproductive tract infection. Women's physical and mental health will also affect the happiness and stability of many families, and then affect the population quality and economic development of the whole society. The common pathogens of female urinary tract infection are Gram-negative bacilli, among which Escherichia coli is the most common, followed by Staphylococcus; most of these are normal flora on the human intestinal tract and skin and mucous membranes. Bacteria can migrate from the intestinal tract, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P13/00A61P15/00A61P31/04C12R1/225
Inventor 朱宝利张瑞芬李晶王江
Owner 北京益生苑生物科技有限公司
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