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Lactobacillus crispatus and application thereof

A technology of Lactobacillus crispatus and Bacillus, applied in the direction of bacteria, antibacterial drugs, antifungal agents, etc., can solve the problems of aggravating vaginal microecological imbalance, BV recurrence, etc., and achieve the effect of strong biofilm formation ability

Active Publication Date: 2018-03-13
内蒙古双奇药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of antibiotics can temporarily alleviate the symptoms of BV, it also further reduces the already reduced Lactobacillus, aggravates the vaginal microecological imbalance, and thus causes BV to recur

Method used

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  • Lactobacillus crispatus and application thereof
  • Lactobacillus crispatus and application thereof
  • Lactobacillus crispatus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Isolation and purification of Lactobacillus crispatus SQ1505

[0021] Under sterile conditions, use an inoculation loop to pick Lactobacillus crispatus SQ1505 and streak it on the MRS plate. The plate is placed in an anaerobic incubator at 37°C for 48hrs, and a single colony is selected for microscopic examination to achieve strain isolation and purification.

Embodiment 2

[0022] Embodiment 2. Fermentation of Lactobacillus crispatus SQ1505

[0023] Use an inoculation loop to pick a ring of Lactobacillus crispatus SQ1505 from the glycerol strain into 10mL MRS liquid medium, culture at 37°C for 14hrs, then insert 4% inoculum into 80mL MRS liquid medium, and culture at 37°C 8hrs, the Lactobacillus crispatus SQ1505 secondary fermentation liquid was obtained.

Embodiment 3

[0024] Example 3. Microscopic examination of Lactobacillus crispatus SQ1505

[0025] The fermentation process of Lactobacillus crispatus SQ1505 of the present invention: culture in MRS liquid medium at 37° C. for 14 hrs. Under sterile conditions, Lactobacillus crispatus SQ1505 was picked with an inoculation loop, spread on a glass slide, fixed and stained with Gram, and the morphology of the bacteria was observed under a microscope.

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Abstract

The invention belongs to the field of probiotics and relates to Lactobacillus crispatus SQ1505. The Lactobacillus crispatus SQ1505 has great performances of acid and hydrogen peroxide production and biofilm formation, has an adhesion effect on human vaginal epithelial cells and an antibacterial effect on vaginal pathogenic bacteria, can be used for prevention and treatment of vaginitis and is a new-generation probiotic capable of replacing antibiotics for preventing and treating female reproductive tract infections.

Description

technical field [0001] The present invention relates to a new bacterial strain of the genus Lactobacillus and its application. More precisely, the present invention describes a new bacterial strain SQ1505 of Lactobacillus crispatus (Lactobacillus crispatus), which has strong ability to produce acid and hydrogen peroxide , It has a strong ability to form biofilm, and has the ability to adhere to human vaginal epithelial cells, and has an antibacterial effect on vaginal pathogenic bacteria, so it has the function of preventing and treating vaginitis. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecosystem of mutual restriction, mutual coordination and dynamic balance with the host and the environment. The vaginal flora of healthy women is mainly composed of Lactobacillus, including Lactobacillus crispatus, Lactobacillus jansneri and Lactobacillus gasseri. Under normal circumstances, Lactobacil...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P15/02A61P31/04A61P31/10C12R1/225
CPCA61K2035/115C12R2001/225C12N1/205Y02A50/30
Inventor 黄少磊刘彦民龚虹王春颖冯谦李康宁李晓军马晓红程超马跃英王海霞邢会霞焦志军王潇郭跃东张春霞韩至赵晓燕海棠
Owner 内蒙古双奇药业股份有限公司
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