The invention belongs to the field of
molecular breeding of crops, and relates to an identifying and utilizing method of a rice wide-compatibility recessive male nuclear sterile line. Nonglutinous rice 'Teqing' and the wide-compatibility variety '02428' are adopted for constructing a recombinant inbred line (RIL), a
selfing line with the
fertility feature is selected, a novel sterile line RI127S is cultivated and screened, and the sterile line can be used as an intermediate material for rice rotational breeding. The identifying and utilizing method has the advantages that (1) the
sterility of the male sterile line is controlled by recessive genes, and is not influenced by length of illumination and temperature under the
normal growth condition, and the male sterile line is an ideal
recurrent selection intermediate material for rice; (2) the sterile line RI127S contains wide-compatibility genes, the
genome of the sterile line RI127S is a mixture of nonglutinous rice and
japonica rice varieties, after hybridization with nonglutinous rice and
japonica rice, the hybridization seeds are fertile, the F1 fruitage rate of nonglutinous rice is 85%, and the F1 fruitage rate of
japonica rice is 80%; (3) the inbred line RI127 has the high yield property intrinsically, and can be taken as a parent of the good stock, and when the RI127 is taken as the sterile line, the process of artificial
emasculation for hybridization can be saved.