Molecular label closely linked with wangshuibai speces and series wheat tibberellin resistance main efect QTL and its application
A wheat scab and molecular marker technology, which is applied in the field of wheat breeding and molecular biology to improve breeding efficiency and reduce waste.
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Embodiment 1
[0022] The acquisition of molecular markers closely linked to the main QTL for wheat head blight resistance:
[0023] (1) Construction of Wangshuibai recombinant inbred line population and identification of red resistance:
[0024] Crossing Wangshuibai with Alondra, a wheat susceptible to head blight, was used to produce F 1 Hybrid, F 1 Selfing produces F 2 Offspring, F 2 One seed of 104 individual plants was randomly selected for planting, and the method of single seed transmission was used to propagate to F 7 Generation, 104 families were produced.
[0025] Fusarium head blight resistance inoculation identification was carried out in the greenhouse or in the field. The spikelets in the middle of the wheat ears were selected and inoculated with 10 microliters (50,000 spores / ml) of conidia suspension of Fusarium graminearum strain F34. After moisturizing for 3 days, the diseased spikelet rate was investigated after 21 days.
[0026] Diseased spikelet rate=(number of diseased spike...
Embodiment 2
[0046] Molecular markers closely linked to the major QTLs for wheat head blight resistance were verified in high-generation genetic populations:
[0047] The obtained molecular markers closely linked to the main QTL for wheat head blight resistance were also predicted for head blight resistance in some plants of the F9 offspring of Wangshuibai crossed with Alondra. DNA was first isolated from their leaves, and then Use SSR markers Xgwm493, BARC147 and Xgwm161 primers to perform polymerase chain reaction (PCR) on these DNAs, and judge whether the corresponding markers are present or not. The presence of 2-3 markers indicates that the variety or strain of scab Resistance reaches the level of "resistance", if it does not exist, it is susceptible. Then based on these criteria, the head blight resistance of each wheat plant was predicted. Subsequently, the method of inoculating the scab pathogen was used to determine the actual resistance to scab of the tested samples and compared with...
Embodiment 3
[0052] Molecular markers linked to the main QTL for red resistance in Wangshuibai predict the resistance of high-generation breeding materials:
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