Method for polymerizing three banded sclerotial blight resistance genes of Lemont and YangDao No.4

A resistance gene, sheath blight technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of not many, sheath blight resistance reduction, etc.

Inactive Publication Date: 2018-05-22
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rice research community has identified many QTLs related to rice sheath blight resistance, but which QTLs jointly regulate rice sheath blight resistance? Which QTLs can be used simultaneously in a breeding process? We don't know much about these issues
According to the molecular markers linked to rice sheath blight resistance genes that have been reported so far, it is often impossible to aggregate several different resistance genes together in order to obtain rice materials with enhanced resistance. Yes, because the randomly selected resistance genes are likely to have epistasis interaction effects between genes, resulting in reduced sheath blight resistance of materials aggregated with multiple resistance genes

Method used

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  • Method for polymerizing three banded sclerotial blight resistance genes of Lemont and YangDao No.4
  • Method for polymerizing three banded sclerotial blight resistance genes of Lemont and YangDao No.4
  • Method for polymerizing three banded sclerotial blight resistance genes of Lemont and YangDao No.4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Example 1: Application of D715, D804, and D1133 markers in the F9 generation segregating population of the hybrid combination of rice variety Lemont and rice variety Yangdao No. 4.

[0009] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao 4 was used as the male parent to cross and construct an F9 generation segregating population (Lemont is a japonica rice variety from the United States, Yangdao 4 is an indica rice variety from Jiangsu, Lemont and Yangdao No. 4 was provided by the National Medium-term Bank of Rice Germplasm Resources of China National Rice Research Institute). This F9 segregation group including 219 strains was sown at the test site of China National Rice Research Institute in Fuyang District, Hangzhou City in May 2015.

[0010] 2. Use the markers D715, D804, and D1133 to perform PCR detection on each of the 219 strains of the recombinant inbred line separation population. Name the band pattern of D715 amplified Yangdao ...

Embodiment 2

[0015] Example 2: Application of the D715, D804, and D1133 markers in the F11 segregating population of the hybrid combination of the rice variety Lemont and the rice variety Yangdao No. 4.

[0016] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross the F11 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao No. 4 was provided by the National Rice Germplasm Bank of China National Rice Research Institute). This F11 segregating population including 219 strains was sown in the experimental farm of China National Rice Research Institute in Fuyang District, Hangzhou City in May 2016.

[0017] 2. Use the markers D715, D804, and D1133 to perform PCR detection on each of the 219 strains of the recombinant inbred line separation population. Name the band pattern of D715 amplified Yangdao No. 4 as A, n...

Embodiment 3

[0022] Example 3: Application of the D715, D804, and D1133 markers in the F13 generation segregating population of the hybrid combination of rice variety Lemont and rice variety Yangdao No. 4.

[0023] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F13 generation segregating population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao No. 4 was provided by the National Rice Germplasm Bank of China National Rice Research Institute). This F13 segregating population including 219 strains was sown in the experimental farm of the China Rice Research Institute in Fuyang District, Hangzhou City in May 2017.

[0024] 2. Use the markers D715, D804, and D1133 to perform PCR detection on each of the 219 strains of the recombinant inbred line separation population. Name the band pattern of D715 amplified Yangdao N...

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Abstract

The invention discloses a method for polymerizing three banded sclerotial blight resistance genes of Lemont and YangDao No.4. The method comprises the following steps: respectively carrying out PCR (Polymerase Chain Reaction) amplification on different strains in an RIL (Recombinant Inbred Line) segregation population formed by the Lemont and the YangDao No.4 by utilizing three markers of D715, D804 and D1133, naming a banding pattern of the YangDao No.4 amplified by the D715 marker as A and the banding pattern of the Lemont amplified by the D715 marker as D, naming the banding pattern of theYangDao No.4 amplified by the D804 marker as B and the banding pattern of the Lemont amplified by the D804 marker as E, and naming the banding pattern of the Lemont amplified by the D1133 marker as Cand the banding pattern of the YangDao No.4 amplified by the D1133 marker as F; selecting the strains with more banding patterns of A, B and C as banded sclerotial blight-resistant strains, and selecting the strains with more banding patterns of D, E and F as banded sclerotial blight-sensitive strains. According to the method disclosed by the invention, banded sclerotial blight-resistant rice materials can be selected by polymerizing three banded sclerotial blight resistance genes of rice at the same time, so that the breeding efficiency can be greatly increased.

Description

Technical field [0001] The present invention relates to a method for polymerizing three sheath blight resistance genes of Lemont and Yangdao No. 4, which can use three molecular markers to simultaneously polymerize three sheath blight resistance genes to select sheath blight resistant genes Rice material. Background technique [0002] Rice is an important food crop related to my country's food security. The traditional methods for breeding rice sheath blight resistant rice in my country's rice breeding community are all through phenotypic selection, that is, using sheath blight pathogens to inoculate individual rice plants at different stages of rice development. Since traditional inoculation identification is mostly carried out under field planting conditions, the results of inoculation identification have large errors and poor accuracy, and there are differences in inoculation results in different years, resulting in low accuracy of inoculation identification results. The sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 曾宇翔杨长登季芝娟梁燕
Owner CHINA NAT RICE RES INST
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