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Method for culturing variety (line) with high phosphorus efficiency by using soybean root configuration near-isogenic line

A near-isogenic line and soybean root technology, applied in the field of genetic improvement and utilization of phosphorus nutrition in design crops, can solve the problems of difficulty in popularization and application, difficulty in obtaining mutant materials, and high cost, so as to improve breeding efficiency and overcome phenotypic selection Difficulty and cascading burdens, the effect of rapid integration

Pending Publication Date: 2018-07-24
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

Although the transgenic technology related to phosphorus nutrition efficiency is conducive to the rapid and accurate transfer of genes related to phosphorus nutrition efficiency, it is difficult to obtain mutant materials in this technical method, the experiment takes a long time, the cost is high, and there are still disputes about product risks in the transgenic technology. , it is more difficult to promote and apply

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  • Method for culturing variety (line) with high phosphorus efficiency by using soybean root configuration near-isogenic line
  • Method for culturing variety (line) with high phosphorus efficiency by using soybean root configuration near-isogenic line
  • Method for culturing variety (line) with high phosphorus efficiency by using soybean root configuration near-isogenic line

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Embodiment Construction

[0033] The method for cultivating phosphorus-efficient varieties (lines) using near-isogenic lines of soybean root configuration is carried out according to the following steps:

[0034] (1) Constructing a recombinant inbred line with root traits related to phosphorus efficiency: BD2, a local variety in Boluo County, Guangdong, with low phosphorus efficiency and deep roots, was used as the female parent, and BX10, a commercial variety introduced from Brazil with high phosphorus efficiency and shallow roots, was used as the male parent. produce F 1 Generation, self-crossing to get F 2 Generation, via single seed transfer method to F 9 Generation (or F 9 generation above), get F 9 Generation recombinant inbred line RIL(F 9 ).

[0035] (2) Mapping of high-efficiency phosphorus QTLs: the above-mentioned F9 generation recombinant inbred line RIL(F 9 ) as the material, respectively in high phosphorus (160kg / hm 2 ), low-phosphorus (no phosphorus application) and low-phosphorus...

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Abstract

The invention discloses a method for culturing a variety (line) with high phosphorus efficiency by using a soybean root configuration near-isogenic line, and belongs to the field of genetic improvement and utilization of phosphorus nutrition of crops. The method comprises main steps of building recombination inbred lines (RILs) of traits of phosphorus efficiency related root systems, building near-isogenic lines (NILs) of efficient root configurations, hybridizing, transferring and screening high-phosphorus-efficiency quantitative trait locuses (QTLs), and the like. The method disclosed by theinvention does not need to depend on a gene cloning and transgenosis technology with high time consumption and high cost, avoids phenomena of difficulty in phenotypic selection, linkage drag and thelike which are caused by conventional hybridization and breeding, realizes fast integration of efficient, high-yield and high-quality genes of the phosphorus nutrition, can obtain a soybean variety (line) with good root configurations and high phosphorus efficiency efficiently and accurately, and greatly improves the breeding efficiency of the variety with high phosphorus efficiency.

Description

technical field [0001] The invention designs the field of genetic improvement and utilization of phosphorus nutrition in crops, and specifically relates to a method for cultivating phosphorus-efficient varieties (lines) by using soybean root structure near isogenes. Background technique [0002] Breeding and popularizing phosphorus-efficient soybean varieties has important ecological and economic significance. Cultivated soybeans〔 Glycine max(L.) Merrill] is an important economic crop and one of the main sources of edible oil and seed protein, which grows in a wide range of soil environments. Phosphorus is one of the three elements of crop nutrients. Phosphorus is a finite mineral nutrient for plants growing in soil. Its availability depends on soil properties and unstable phosphorus content. It is estimated that in 2 / 3 of the cultivated soils in the world, the available phosphorus is generally low, and there are different degrees of phosphorus deficiency. When phosph...

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Application Information

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IPC IPC(8): A01H1/02C12Q1/6895
CPCA01H1/02C12Q1/6895C12Q2600/156
Inventor 梁泉尹元萍董文汉张雅琼张慧
Owner YUNNAN AGRICULTURAL UNIVERSITY
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