Method for constructing QTL positioned new-group-linkage F2 group

Abstract

The invention discloses a method for constructing a QTL positioned new-group-linkage F2 group.The method comprises the steps of selecting three selfing lines which are from different sources and have great genetic background differences, utilizing the three selfing lines for hybridization to obtain three hybrids, adopting a single-grain genetic method for performing selfing on the three hybrids for seven generations or more till all the lines in the group are purified, and selecting and breeding three recombination selfing lines; constructing a linkage F2 group, performing recombination on every two selfing lines, and performing hybridization on every two extracted lines randomly to obtain n combinations; finally, obtaining three F2 groups, and forming a linkage F2 group, wherein the number of the hybridization combinations of the obtained linkage F2 group is 3n.According to the constructed linkage F2 group, QTL positioning is researched, the advantages of a temporary group and the advantages of a permanent group are synthesized, meanwhile, the defect existing in QTL positioning of the permanent F2 group is overcome, the accuracy of QTL positioning tests is improved, and the group is novel and can precisely position QTL and promote forward development of hybrid advantage theory research.

Description

technical field [0001] The invention belongs to the technical field of modern biological science research, and relates to multiple research fields such as molecular genetics and quantitative genetics. Scientificity, reliability of experimental data, characteristics of different QTL analysis methods and many other factors. Background technique [0002] Most of the important agronomic traits of crops are controlled by multiple genes, and there are complex interactions between genes, and the expression of genes is also affected by environmental interactions. Therefore, accurately locating the position of a gene controlling a quantitative trait in the genome (QTL mapping) is of great significance for the study of crop genetic mechanism and heterosis. [0003] QTL mapping is to locate the QTL on the genetic map by analyzing the relationship between the DNA molecular markers of the entire chromosome set and the phenotypic value of quantitative traits, and to determine the distanc...

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Problems solved by technology

[0019] Temporary group materials are easy to obtain, but it is difficult to conduct continuous research on them. There is no guarantee that there are enough materials, and the reliability of the data cannot be repeated. The limited materials make it difficult for multiple laboratories to conduct research or multi-environment interaction research material needs cannot be met

The permanent population is characterized by homozygous or near-homozygous materials within the population, lack of heterozygous types, and it is impossible to estimate QTL dominance effects, epistasis effects and environmental interaction effects related to dominance

Permanent F 2 The population is obtained by crossing each homozygous line in the permanent population according to a certain combination scheme, both with F 2 The group has the advantages of large amount of information, can estimate the dominant effect and the epistasis effect related to the dominant, and can assemble enough seeds to meet the needs of multi-year multi-point experiments to obtain accurate phenotypic observations, which is conducive to close identification The advantages of linked QTL markers; however, the genetic background between RILs used for assembly is the same, resulting in RILs after multiple generations of selfing S The loss probability of the same gene is greatly increased, and the number of QTL mapping for the same trait is limited, especially it is difficult to locate the gene loci with strong heterosis

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