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Primers and kit for detecting molecular marker linked to major QTL for controlling corn stalk strength, detection method for detecting corn stalk strength, and applications of primers, kit and detection method

A detection control and molecular marker technology, applied in the fields of corn genetics and molecular biology, can solve the problems of inconsistent QTL, large positioning interval, and limited utilization efficiency

Inactive Publication Date: 2019-02-01
河南省农业科学院作物设计中心
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In recent years, researchers have used different genetic groups to map the QTL of maize stalk strength. The QTLs detected in different environments are not consistent, and the location interval is large, which limits its utilization efficiency in breeding.

Method used

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  • Primers and kit for detecting molecular marker linked to major QTL for controlling corn stalk strength, detection method for detecting corn stalk strength, and applications of primers, kit and detection method
  • Primers and kit for detecting molecular marker linked to major QTL for controlling corn stalk strength, detection method for detecting corn stalk strength, and applications of primers, kit and detection method
  • Primers and kit for detecting molecular marker linked to major QTL for controlling corn stalk strength, detection method for detecting corn stalk strength, and applications of primers, kit and detection method

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Embodiment 1

[0036] In this example, the primers for the detection of molecular markers linked to the major QTL controlling maize stalk strength, the primer sequences are as follows:

[0037] F1: 5'-ACCGACCTAAGCTATGGGCT-3' (SEQ ID NO.1);

[0038] R1: 5'-CCGGTTATAAACACAGCCGT-3' (SEQ ID NO. 2).

[0039] Example 1 of a kit for detecting molecular markers linked to major QTLs controlling corn stalk strength

[0040]The kit for detecting the molecular markers linked to the main QTL controlling the corn stalk strength in this example, including primers, Taq Master Mix, buffer, dNTP, ddH 2 O, the sequence of the primer is as follows:

[0041] F1: 5'-ACCGACCTAAGCTATGGGCT-3' (SEQ ID NO.1);

[0042] R1: 5'-CCGGTTATAAACACAGCCGT-3' (SEQ ID NO. 2).

[0043] Example 1 of the method for detecting the stalk strength of corn lines

[0044] The method for detecting the stalk strength of corn lines in this embodiment comprises the following steps:

[0045] (1) Extraction of corn genomic DNA

[0046] A...

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Abstract

The invention relates to primers and a kit for detecting a molecular marker linked to major QTL for controlling corn stalk strength, a detection method for detecting corn stalk strength, and applications of the primers, the kit and the detection method, and belongs to the technical field of corn genetic breeding and molecular biology. According to the present invention, a recombinant selfing linepopulation containing 241 families is constructed through Zheng 58*D863F, and genetic linkage map analysis is performed, such that four QTL for controlling corn stalk strength are detected, wherein the high stalk strength major QTL qRPR1.07 is identified simultaneously at the position 1.07 of the chromosome 1 of the corn from Hainan Ledong and Henan Yuanyang Demonstration Base, and is positioned between two SSR markers bnlg1556 and umc2232 so as to explain nearly 20% of genetic variation; and the molecular marker linked to the major QTL for controlling corn stalk strength can directionally andprecisely screen corn materials with high stalk strength at the early stage of growth so as to save the breeding cost and the breeding time, and is used for lodging-resistant breeding of corn.

Description

technical field [0001] The invention relates to a primer, a kit, a detection method and an application for detecting a molecular marker linked to a main-effect QTL controlling corn stalk strength, and belongs to the technical fields of corn genetics and breeding and molecular biology. Background technique [0002] Corn is one of the most important food crops in my country. In recent years, with the improvement of the scale of corn production, the requirement of corn varieties to adapt to the whole process of mechanization has become more and more urgent. At present, the low level of mechanical harvesting is a "bottleneck" encountered in the development of corn mechanization, and corn stalk lodging is one of the main factors restricting the development of corn mechanized harvesting in my country; at the same time, with the increasing frequency of extreme climates in the world , the requirements for stable yield and lodging resistance of corn varieties are getting higher and hi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王新涛杨青代资举李保全王艳张莹莹
Owner 河南省农业科学院作物设计中心
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