Molecular marker separated from low-polyphenol-oxidase-activity gene QPpo-5D of wheat
A technology of phenol oxidase activity and polyphenol oxidase, which is applied in the field of agricultural biology, can solve the problem that there is no low PPO active site, and achieve the effects of improving breeding efficiency, speeding up the breeding process, and simplifying the selection method.
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Embodiment 1
[0034] Example 1. Acquisition of Molecular Markers Linked to IDO580-21 Wheat Low PPO Activity Gene
[0035] 1. Plant material
[0036] Yangmai 158 (Suzhongshenzi No. 179) was used as a control with high PPO activity. IDO580-21 ('Cadoux'(PI591905) / / 'Maya74' / M2) was preserved and provided by the Germplasm Resource Evaluation and Innovation Laboratory of the Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, and its grain PPO activity is extremely low . The hybrid combination of IDO580-21 and Yangmai 158 was prepared to construct a recombinant inbred line population.
[0037] 2. Grain PPO activity evaluation method
[0038] In this study, dopa (L-DOPA / MOPS) was used as the substrate, and PPO catalyzed L-DOPA to form quinones, which in turn caused browning. For each material, we selected plump, undamaged, and disease-free grains, weighed them, and put them into 96-well microtiter plates, with 1 grain per well. Eight replicates and a b...
Embodiment 2
[0054] Example 2, the application of molecular marker AX-110939270 in varieties (lines) in the middle and lower reaches of the Yangtze River
[0055] In order to verify the allelic distribution of the AX-110939270 marker in the varieties (lines) in the middle and lower reaches of the Yangtze River, and whether it can be applied to the breeding improvement of the varieties (lines) in the middle and lower reaches of the Yangtze River, this study used the AX-110939270 marker to analyze 53 accessions from the middle and lower reaches of the Yangtze River. Varieties (lines) were screened, and fluorescence scanning results were obtained by reading the fluorescence signals of KASP-labeled amplified products, and the scanning data were analyzed by Kluster Caller software, showing that these varieties contained heterozygous and homozygous high and low PPO activity genotypes. The variety in the X-axis area of the scatter plot is the "FAM" genotype, the variety in the Y-axis area is the...
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