Rice variety IR36 black-streaked dwarf virus resistance site qRBSDV-1 and molecular marking method and application thereof

Abstract

The invention relates to a rice variety IR36 black-streaked dwarf virus resistance site qRBSDV-1 and a molecular marking method and application thereof. According to the invention, a recombinant inbred line population constructed by crossing a rice black-streaked dwarf virus resistance variety IR36 and a black-streaked dwarf virus susceptible variety L5494 is taken as a positioning population, andthe black-streaked dwarf virus resistance identification is carried out by a field natural identification method, a resistance gene site, named qRBSDV-1, from a black-streaked dwarf virus resistancevariety IR36 is obtained, located between the markers AP-39.6 and RM104. The method verifies qRBSDV-1 by performing molecular detection and identification of the incidence of black-streaked dwarf virus by using a substitution line (No. N9) containing 93-11 introduced fragments only in the chromosome segment where qRBSDV-1 is located and parents in a Japanese sunny background. According to the invention, the resistance of the plant to the rice black-streaked dwarf virus can be predicted by detecting the bands at the molecular markers of AP-39.6 and RM104, and the breeding efficiency of the ricevariety resistant to the black-streaked dwarf virus is improved.

Description

technical field [0001] The invention relates to the identification of qRBSDV-1 locus resistant to black-streaked dwarf disease in rice variety IR36 and its molecular marker method and application, belonging to the field of rice molecular breeding science and technology. Background technique [0002] Rice black-streaked dwarf disease is a viral disease caused by rice black-streaked dwarfvirus (RBSDV), which is mainly transmitted by persistent non-oval means by the small planthopper. The typical symptom of rice black-streaked dwarf disease is that the diseased plant is dwarfed, the leaf color is dark green and stiff, and the leaf back, leaf sheath and stem have short irregular protrusions that are waxy white in the early stage and dark brown in the later stage. Usually the affected plants do not head Or very little fruit, known as the "cancer" of rice. In the 1960s, rice black-streaked dwarf disease broke out in various provinces and cities in East my country. In the followin...

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Problems solved by technology

[0004] In order to overcome the above-mentioned defects, the present invention provides the black-streaked dwarf resistance locus qRBSDV-1 in rice variety IR36 for the shortcomings of rice black-streaked dwarf resistance identification stability, poor repeatability, time-consuming and labor-intensive problems in conventional breeding And its molecular marker method, which can predict the resistance of plants to rice black-streaked dwarf disease, simplifies the selection method, and accelerates the breeding process of disease-resistant varieties

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