A SNP Molecular Marker of Jute Anthracnose Resistance Gene Segment

A technology of molecular markers and resistance genes, which is applied in the direction of recombinant DNA technology, microbial detection/testing, DNA/RNA fragments, etc., can solve the problems of reducing jute yield and fiber quality, etc.

Inactive Publication Date: 2018-03-09
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pests and diseases are known to significantly reduce jute yield and fiber quality

Method used

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  • A SNP Molecular Marker of Jute Anthracnose Resistance Gene Segment
  • A SNP Molecular Marker of Jute Anthracnose Resistance Gene Segment
  • A SNP Molecular Marker of Jute Anthracnose Resistance Gene Segment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1: 26 SNP molecular markers linked to anthrax resistance genes were developed, such as figure 2 shown.

Embodiment 2

[0077] Example 2: Using the SNP molecular markers developed above for jute resistance germplasm screening. The test materials were 144 jute lines. 18 disease-resistant strains numbered 98, 45, 76, 122, 124, 20, 101, and 27 were screened out, accounting for 12.5% ​​of the total. It shows that there are obvious differences in the resistance to anthracnose among different jute strains. In order to further confirm the accuracy of field inoculation, 7 strains with different resistances were selected for inoculation identification in the greenhouse. Investigating its incidence, it was found that there was little difference from field inoculation.

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Abstract

The invention belongs to the technical field of breeding of corchorus spp. and relates to an SNP molecular marker in a corchorus spp. anthracnose resistant gene segment. The SNP molecular marker is realized by the following steps: determining the genetype by extraction of a genome DNA of a corchorus spp. recombinant inbred line (RIL) population, digestion, product purification sequencing, and SNP screening and analysis; identifying the resistant character of the corchorus spp. recombinant inbred line (RIL) population by adopting a single factor randomized block design; and performing genetic linkage map establishment and QTL (quantitative trait loci) positioning on the recombinant inbred line population, wherein the QTL positioning result shows that the phenotypic variation explained by QTL qAR.N10 is 20.13%, the QTL is a major QTL, the QTL segment comprises 26 SNP molecular markers, and the nucleotide sequences and variation sites of the 26 SNP molecular markers are as shown in SEQ ID NO.1-26. The invention provides a new molecular marker for resistance breeding of corchorus spp. anthracnose, which can be used for assisted selection of molecular markers of corchorus spp. anthracnose.

Description

technical field [0001] The invention belongs to the technical field of jute breeding and relates to a SNP molecular marker of a jute anthracnose resistance gene segment. Background technique [0002] Single nucleotide polymorphisms (SNP, Single Nucleotide Polymorphisms) refer to genetic markers formed by the variation of a single nucleotide on the genome, including transitions, transversions, deletions / insertions, etc. Its number is large and its polymorphism is rich. Theoretically, each SNP site can have 4 different variant forms. SNPs appear more frequently in CG sequences, and most of them are converted from C to T. This is because cytosine (C) in CG sequences is often methylated, and then spontaneously deaminated to become thymine (T). SNP is the third-generation genetic marker, and many differences in important agronomic traits of crops may be related to SNP. SNP is widely used in genetic map construction, gene mapping, variety identification and other fields. For e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张立武祁建民高红陶爱芬徐建堂林荔辉方平平林培清
Owner FUJIAN AGRI & FORESTRY UNIV
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