The invention discloses a quick manufacturing method of a
myocardial infarction model in rats. After a rat is subjected to induced
anesthesia, a breath
facial mask can continuously perform
anesthesia,blunt separation on
pectoralis major muscle and pectoral minor
muscle of left front chest is carried out, and a
thoracic cavity is opened between a third rib and a
fourth rib; through circulating andcooperative operation of a
thumb, an index and a
middle finger of an operator, a free end of a heart of the rat is extruded out of the
chest cavity and then left ventricular anterior descending
artery is ligated. By adopting the method, the
myocardial infarction model in the rats is manufactured,
endotracheal intubation is not carried out, and the time for
surgery is 30 seconds to 60 seconds, thechest cavity opening time is 15 seconds to 30 seconds, the air of the
chest cavity is squeezed manually through operation of the operator when the chest is closed, the negative pressure of the chestcavity is maintained, spontaneous
breathing of the rat cannot be affected, aerothorax and difficulty
breathing are avoided, the rat is out of the
anesthesia for 50 seconds to 90 seconds after the surgerey, and then inhibition of a
central nervous system can be released; and a success ratio of the
myocardial infarction model is high, and a death ratio is low. According to the method disclosed by the invention, the manufacturing time of the myocardial
infarction model in the rats is shortened, and the experimental efficiency during massive using of the myocardial
infarction model in the rats isimproved.