The invention discloses a
liquid phase nanometer
colloidal gold colorimetric method for quickly detecting malignant tumor
DNA (deoxyribonucleic acid), and belongs to the technical field of malignant
tumor detection methods. The method has the technical characteristics that a
glass container used for an experiment is cleaned with detergent, after the
glass container finishes being cleaned with thedetergent, the
glass container is cleaned with
distilled water, then, the glass container is dipped in silicified
reagent overnight, the inner surface of a gold manufacture vessel is subjected to silicified
processing, and the glass container is cleaned with
distilled water for standby; 1%(w / v)
chloroauric acid and 1%(w / v)
sodium citrate solution are prepared, and a filter membrane of which the aperture is 0.2
micrometer is used for filtering; and one magnetic force stirrer is put in a 1000mL of round-bottom flask which is cleaned, and 1000mL of
ultrapure water is added. By use of the method,according to the color change of nanometer
colloidal gold solution, whether
DNA methylation is normal or not can be represented so as to bring convenience for medical personnel in making an early diagnosis for the malignant tumor before clinical symptoms appear, and therefore, the method is favorable for promotion and use.