Personalized, economical and practical tumor therapeutic effect evaluation and relapse monitoring method

Abstract

The invention provides a non-invasive tumor therapeutic effect evaluation and relapse monitoring method which is economical, practical, high in sensitivity and effective for each individual. The method includes the steps of one-time high-flux sequencing screening and real-time fluorescence PCR daily non-invasive monitoring. Firstly, tumor DNA is extracted from tumor tissue or blood or marrow of individuals, sequencing is conducted through a high-flux sequencing instrument, proper detection sites are screened according to a screening standard provided by the method, a real-time fluorescence PCR method is established, and the relapse conditions of tumors are monitored and therapeutic effect evaluation is conducted through existence of the detection sites in the blood. The bodies of the patients are searched for existing mutational sites through a high-flux sequencing method, and it can be guaranteed that the method is effective for each individual. The detection sites screened according to the special screening standard provided by the method can be detected in the background of a large amount of normal DNA, and sensitivity is high. The real-time fluorescence PCR method serves as a means for daily monitoring tumor mutant genes in the blood or urine, and the method is economical, practical and free of trauma.

Description

technical field [0001] The invention relates to a method of using high-throughput sequencing to find personalized mutation sites of tumor patients, and daily monitoring of these mutation sites from blood and urine by real-time fluorescent PCR method, so as to realize tumor curative effect evaluation and recurrence monitoring method, which belongs to transformation Medical field. Background technique [0002] The most noteworthy issues in the course of tumor treatment are treatment efficacy evaluation and recurrence monitoring. At present, the clinical evaluation of the curative effect of solid tumors and the monitoring of recurrence mainly adopt imaging methods such as B-ultrasound and MRI. This method needs to be used for curative effect evaluation to evaluate the curative effect after several months of treatment, and is not sensitive enough; it is not sensitive enough for recurrence When monitoring, it is often not discovered until the tumor grows to a certain size, and i...

AI Technical Summary

Problems solved by technology

[0011] The above table shows that the incidence of gene rearrangement and translocation in the tumor population is too low to be widely adopted

[0012] Although high-throughput sequencing can also be used to detect circulating tumor DNA in blood when the concentration of circulating tumor DNA is high, the current high-throughput sequencing is not suitable for the high cost, long cycle, complicated operation, and lack of standards for data analysis. as a means of daily monitoring

[0013] In summary, there is no report of an economical and practical molecular biological method that is suitable for each individual, has high sensitivity, and can use blood or urine for non-invasive tumor recurrence monitoring and efficacy evaluation

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