Liquid phase nanometer colloidal gold colorimetric method for quickly detecting malignant tumor DNA (deoxyribonucleic acid)
A nano-colloid and malignant tumor technology, applied in the field of malignant tumor detection, can solve the problems of disordered proliferation of malignant tumor cells, malignant tumors, abnormal cell metabolism, etc., and achieve the effect of promoting the use
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Embodiment 1
[0020] A liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA, the steps are as follows:
[0021] S1. Wash the glass container used in the experiment with detergent. After washing with detergent, rinse it with distilled water, then soak it overnight with a siliconizing agent, perform siliconization treatment on the inner surface of the gold vessel, and rinse it with distilled water. ,spare;
[0022] S2, prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution, and use a filter membrane with a pore size of 0.2 microns to filter;
[0023] S3, put 1 magnetic stirrer in the cleaned 1000mL round bottom flask, add 1000mL ultrapure water;
[0024] S4, adding concentration to the round bottom flask is 1% (w / v) chloroauric acid, the stirring rate is 600rpm, heat the solution in the in situ flask to boiling during the stirring process, then add the concentration of 1:1 volume of chloroauric acid Put 1% sodium citrate solution int...
Embodiment 2
[0028] A liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA, the steps are as follows:
[0029] S1. Wash the glass container used in the experiment with detergent. After washing with detergent, rinse it with distilled water, then soak it overnight with a siliconizing agent, perform siliconization treatment on the inner surface of the gold vessel, and rinse it with distilled water. ,spare;
[0030] S2, prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution, and use a filter membrane with a pore size of 0.2 microns to filter;
[0031] S3, put 1 magnetic stirrer in the cleaned 1000mL round bottom flask, add 1000mL ultrapure water;
[0032] S4, adding concentration to the round bottom flask is 1% (w / v) chloroauric acid, the stirring rate is 600rpm, heat the solution in the in situ flask to boiling during the stirring process, then add the concentration of 1:1 volume of chloroauric acid Put 1% sodium citrate solution int...
Embodiment 3
[0036] A liquid-phase nano colloidal gold colorimetric method for rapidly detecting malignant tumor DNA, the steps are as follows:
[0037] S1. Wash the glass container used in the experiment with detergent. After washing with detergent, rinse it with distilled water, then soak it overnight with a siliconizing agent, perform siliconization treatment on the inner surface of the gold vessel, and rinse it with distilled water. ,spare;
[0038] S2, prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution, and use a filter membrane with a pore size of 0.2 microns to filter;
[0039] S3, put 1 magnetic stirrer in the cleaned 1000mL round bottom flask, add 1000mL ultrapure water;
[0040] S4, adding concentration to the round bottom flask is 1% (w / v) chloroauric acid, the stirring rate is 600rpm, heat the solution in the in situ flask to boiling during the stirring process, then add the concentration of 1:1 volume of chloroauric acid Put 1% sodium citrate solution int...
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